Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11355 and AMB_RS11360 are separated by 160 nucleotides AMB_RS11360 and AMB_RS11365 are separated by 177 nucleotides AMB_RS11365 and AMB_RS11370 are separated by 88 nucleotides
AMB_RS11355: AMB_RS11355 - protein-L-isoaspartate O-methyltransferase, at 2,446,864 to 2,447,520
_RS11355
AMB_RS11360: AMB_RS11360 - membrane protein, at 2,447,681 to 2,449,054
_RS11360
AMB_RS11365: AMB_RS11365 - DUF2497 domain-containing protein, at 2,449,232 to 2,449,927
_RS11365
AMB_RS11370: AMB_RS11370 - valine--tRNA ligase, at 2,450,016 to 2,452,685
_RS11370
Position (kb)
2447
2448
2449
2450 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2446.778 kb on + strand at 2446.779 kb on - strand at 2446.870 kb on + strand at 2446.871 kb on - strand at 2446.890 kb on + strand at 2446.938 kb on - strand, within AMB_RS11355 at 2446.976 kb on + strand, within AMB_RS11355 at 2446.977 kb on - strand, within AMB_RS11355 at 2447.002 kb on + strand, within AMB_RS11355 at 2447.003 kb on - strand, within AMB_RS11355 at 2447.148 kb on - strand, within AMB_RS11355 at 2447.249 kb on - strand, within AMB_RS11355 at 2447.249 kb on - strand, within AMB_RS11355 at 2447.249 kb on - strand, within AMB_RS11355 at 2447.249 kb on - strand, within AMB_RS11355 at 2447.293 kb on + strand, within AMB_RS11355 at 2447.293 kb on + strand, within AMB_RS11355 at 2447.293 kb on + strand, within AMB_RS11355 at 2447.294 kb on - strand, within AMB_RS11355 at 2447.295 kb on + strand, within AMB_RS11355 at 2447.295 kb on + strand, within AMB_RS11355 at 2447.423 kb on + strand, within AMB_RS11355 at 2447.423 kb on + strand, within AMB_RS11355 at 2447.423 kb on + strand, within AMB_RS11355 at 2447.424 kb on - strand, within AMB_RS11355 at 2447.439 kb on + strand, within AMB_RS11355 at 2447.440 kb on - strand, within AMB_RS11355 at 2447.615 kb on + strand at 2447.631 kb on + strand at 2447.633 kb on + strand at 2447.634 kb on - strand at 2447.669 kb on + strand at 2447.691 kb on + strand at 2447.692 kb on - strand at 2447.783 kb on + strand at 2447.784 kb on - strand at 2447.784 kb on - strand at 2447.831 kb on - strand, within AMB_RS11360 at 2447.910 kb on - strand, within AMB_RS11360 at 2448.129 kb on + strand, within AMB_RS11360 at 2448.309 kb on - strand, within AMB_RS11360 at 2448.317 kb on - strand, within AMB_RS11360 at 2448.317 kb on - strand, within AMB_RS11360 at 2448.640 kb on + strand, within AMB_RS11360 at 2448.834 kb on + strand, within AMB_RS11360 at 2448.834 kb on + strand, within AMB_RS11360 at 2448.834 kb on + strand, within AMB_RS11360 at 2448.834 kb on + strand, within AMB_RS11360 at 2448.834 kb on + strand, within AMB_RS11360 at 2448.835 kb on - strand, within AMB_RS11360 at 2448.835 kb on - strand, within AMB_RS11360 at 2448.911 kb on + strand, within AMB_RS11360 at 2448.911 kb on + strand, within AMB_RS11360 at 2448.911 kb on + strand, within AMB_RS11360 at 2448.974 kb on + strand at 2448.974 kb on + strand at 2448.992 kb on + strand at 2449.046 kb on + strand at 2449.052 kb on + strand at 2449.189 kb on + strand at 2449.922 kb on + strand at 2449.925 kb on + strand at 2449.941 kb on + strand at 2449.941 kb on + strand at 2449.941 kb on + strand at 2449.941 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,446,778 + +0.4 2,446,779 - -0.3 2,446,870 + -1.1 2,446,871 - -0.2 2,446,890 + -1.7 2,446,938 - AMB_RS11355 0.11 -0.8 2,446,976 + AMB_RS11355 0.17 -1.5 2,446,977 - AMB_RS11355 0.17 -0.8 2,447,002 + AMB_RS11355 0.21 -0.3 2,447,003 - AMB_RS11355 0.21 -1.2 2,447,148 - AMB_RS11355 0.43 +0.3 2,447,249 - AMB_RS11355 0.59 +1.3 2,447,249 - AMB_RS11355 0.59 +0.0 2,447,249 - AMB_RS11355 0.59 -0.8 2,447,249 - AMB_RS11355 0.59 -0.1 2,447,293 + AMB_RS11355 0.65 +0.5 2,447,293 + AMB_RS11355 0.65 -0.8 2,447,293 + AMB_RS11355 0.65 -2.0 2,447,294 - AMB_RS11355 0.65 +0.5 2,447,295 + AMB_RS11355 0.66 +0.5 2,447,295 + AMB_RS11355 0.66 -0.7 2,447,423 + AMB_RS11355 0.85 -1.1 2,447,423 + AMB_RS11355 0.85 -1.0 2,447,423 + AMB_RS11355 0.85 -0.0 2,447,424 - AMB_RS11355 0.85 +0.1 2,447,439 + AMB_RS11355 0.88 -0.7 2,447,440 - AMB_RS11355 0.88 +0.3 2,447,615 + -0.1 2,447,631 + +1.3 2,447,633 + -1.7 2,447,634 - -0.7 2,447,669 + +1.3 2,447,691 + -1.0 2,447,692 - -2.5 2,447,783 + -2.2 2,447,784 - -1.2 2,447,784 - +0.3 2,447,831 - AMB_RS11360 0.11 -0.0 2,447,910 - AMB_RS11360 0.17 -0.8 2,448,129 + AMB_RS11360 0.33 -1.7 2,448,309 - AMB_RS11360 0.46 -0.7 2,448,317 - AMB_RS11360 0.46 -1.8 2,448,317 - AMB_RS11360 0.46 -0.3 2,448,640 + AMB_RS11360 0.70 -0.2 2,448,834 + AMB_RS11360 0.84 +0.3 2,448,834 + AMB_RS11360 0.84 -3.0 2,448,834 + AMB_RS11360 0.84 -0.3 2,448,834 + AMB_RS11360 0.84 -0.2 2,448,834 + AMB_RS11360 0.84 -0.2 2,448,835 - AMB_RS11360 0.84 -3.2 2,448,835 - AMB_RS11360 0.84 -1.2 2,448,911 + AMB_RS11360 0.90 -0.0 2,448,911 + AMB_RS11360 0.90 -1.0 2,448,911 + AMB_RS11360 0.90 +0.1 2,448,974 + -0.4 2,448,974 + -2.1 2,448,992 + +1.3 2,449,046 + -1.8 2,449,052 + +0.3 2,449,189 + -0.5 2,449,922 + +0.3 2,449,925 + +0.3 2,449,941 + -1.1 2,449,941 + -2.5 2,449,941 + -0.5 2,449,941 + +0.3
Or see this region's nucleotide sequence