Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11255 and AMB_RS11260 overlap by 4 nucleotides AMB_RS11260 and AMB_RS11265 are separated by 9 nucleotides AMB_RS11265 and AMB_RS11270 overlap by 16 nucleotides AMB_RS11270 and AMB_RS11275 overlap by 8 nucleotides AMB_RS11275 and AMB_RS11280 are separated by 156 nucleotides
AMB_RS11255: AMB_RS11255 - hydrogenase iron-sulfur subunit, at 2,423,341 to 2,424,915
_RS11255
AMB_RS11260: AMB_RS11260 - hypothetical protein, at 2,424,912 to 2,425,415
_RS11260
AMB_RS11265: AMB_RS11265 - hypothetical protein, at 2,425,425 to 2,426,471
_RS11265
AMB_RS11270: AMB_RS11270 - hypothetical protein, at 2,426,456 to 2,426,779
_RS11270
AMB_RS11275: AMB_RS11275 - Heme/copper-type cytochrome/quinol oxidase, at 2,426,772 to 2,427,257
_RS11275
AMB_RS11280: AMB_RS11280 - beta-glucosidase, at 2,427,414 to 2,428,763
_RS11280
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2424.634 kb on + strand, within AMB_RS11255 at 2424.634 kb on + strand, within AMB_RS11255 at 2424.634 kb on + strand, within AMB_RS11255 at 2424.635 kb on - strand, within AMB_RS11255 at 2424.700 kb on + strand, within AMB_RS11255 at 2424.700 kb on + strand, within AMB_RS11255 at 2424.930 kb on - strand at 2424.930 kb on - strand at 2424.952 kb on - strand at 2424.952 kb on - strand at 2424.952 kb on - strand at 2424.952 kb on - strand at 2424.954 kb on - strand at 2424.978 kb on + strand, within AMB_RS11260 at 2424.979 kb on - strand, within AMB_RS11260 at 2425.115 kb on + strand, within AMB_RS11260 at 2425.115 kb on + strand, within AMB_RS11260 at 2425.115 kb on + strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.116 kb on - strand, within AMB_RS11260 at 2425.413 kb on + strand at 2425.413 kb on + strand at 2425.413 kb on + strand at 2425.800 kb on + strand, within AMB_RS11265 at 2425.800 kb on + strand, within AMB_RS11265 at 2425.800 kb on + strand, within AMB_RS11265 at 2425.801 kb on - strand, within AMB_RS11265 at 2425.801 kb on - strand, within AMB_RS11265 at 2425.801 kb on - strand, within AMB_RS11265 at 2425.801 kb on - strand, within AMB_RS11265 at 2425.801 kb on - strand, within AMB_RS11265 at 2425.801 kb on - strand, within AMB_RS11265 at 2425.830 kb on + strand, within AMB_RS11265 at 2425.830 kb on + strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.831 kb on - strand, within AMB_RS11265 at 2425.833 kb on - strand, within AMB_RS11265 at 2425.833 kb on - strand, within AMB_RS11265 at 2425.944 kb on + strand, within AMB_RS11265 at 2425.944 kb on + strand, within AMB_RS11265 at 2425.945 kb on - strand, within AMB_RS11265 at 2425.945 kb on - strand, within AMB_RS11265 at 2426.194 kb on - strand, within AMB_RS11265 at 2426.194 kb on - strand, within AMB_RS11265 at 2426.283 kb on - strand, within AMB_RS11265 at 2426.283 kb on - strand, within AMB_RS11265 at 2426.435 kb on + strand at 2426.548 kb on - strand, within AMB_RS11270 at 2426.778 kb on - strand at 2426.880 kb on + strand, within AMB_RS11275 at 2426.881 kb on - strand, within AMB_RS11275 at 2427.057 kb on + strand, within AMB_RS11275 at 2427.058 kb on - strand, within AMB_RS11275 at 2427.058 kb on - strand, within AMB_RS11275 at 2427.198 kb on + strand, within AMB_RS11275 at 2427.199 kb on - strand, within AMB_RS11275 at 2427.314 kb on + strand at 2427.315 kb on - strand at 2427.350 kb on + strand at 2427.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,424,634 + AMB_RS11255 0.82 -3.1 2,424,634 + AMB_RS11255 0.82 +0.1 2,424,634 + AMB_RS11255 0.82 -0.7 2,424,635 - AMB_RS11255 0.82 +0.4 2,424,700 + AMB_RS11255 0.86 -0.2 2,424,700 + AMB_RS11255 0.86 +0.1 2,424,930 - -1.5 2,424,930 - -0.8 2,424,952 - -0.1 2,424,952 - -0.0 2,424,952 - -0.7 2,424,952 - -1.2 2,424,954 - -1.8 2,424,978 + AMB_RS11260 0.13 +0.0 2,424,979 - AMB_RS11260 0.13 -1.2 2,425,115 + AMB_RS11260 0.40 -0.0 2,425,115 + AMB_RS11260 0.40 -1.7 2,425,115 + AMB_RS11260 0.40 -0.1 2,425,116 - AMB_RS11260 0.40 -1.2 2,425,116 - AMB_RS11260 0.40 -0.5 2,425,116 - AMB_RS11260 0.40 +0.5 2,425,116 - AMB_RS11260 0.40 +0.5 2,425,116 - AMB_RS11260 0.40 -1.0 2,425,116 - AMB_RS11260 0.40 -0.5 2,425,116 - AMB_RS11260 0.40 +0.8 2,425,413 + +0.2 2,425,413 + +0.1 2,425,413 + -0.3 2,425,800 + AMB_RS11265 0.36 -3.0 2,425,800 + AMB_RS11265 0.36 +0.3 2,425,800 + AMB_RS11265 0.36 -0.2 2,425,801 - AMB_RS11265 0.36 +0.3 2,425,801 - AMB_RS11265 0.36 -0.0 2,425,801 - AMB_RS11265 0.36 -0.2 2,425,801 - AMB_RS11265 0.36 -0.8 2,425,801 - AMB_RS11265 0.36 -2.2 2,425,801 - AMB_RS11265 0.36 +0.3 2,425,830 + AMB_RS11265 0.39 +0.2 2,425,830 + AMB_RS11265 0.39 -1.0 2,425,831 - AMB_RS11265 0.39 -0.6 2,425,831 - AMB_RS11265 0.39 +0.0 2,425,831 - AMB_RS11265 0.39 +0.1 2,425,831 - AMB_RS11265 0.39 -2.1 2,425,831 - AMB_RS11265 0.39 -0.3 2,425,831 - AMB_RS11265 0.39 -0.6 2,425,831 - AMB_RS11265 0.39 +0.7 2,425,831 - AMB_RS11265 0.39 -0.0 2,425,831 - AMB_RS11265 0.39 -0.9 2,425,833 - AMB_RS11265 0.39 -0.7 2,425,833 - AMB_RS11265 0.39 -0.3 2,425,944 + AMB_RS11265 0.50 -1.2 2,425,944 + AMB_RS11265 0.50 -0.2 2,425,945 - AMB_RS11265 0.50 -0.4 2,425,945 - AMB_RS11265 0.50 -0.3 2,426,194 - AMB_RS11265 0.73 -2.1 2,426,194 - AMB_RS11265 0.73 +0.2 2,426,283 - AMB_RS11265 0.82 -1.9 2,426,283 - AMB_RS11265 0.82 -0.1 2,426,435 + +0.6 2,426,548 - AMB_RS11270 0.28 -1.2 2,426,778 - +0.3 2,426,880 + AMB_RS11275 0.22 +0.5 2,426,881 - AMB_RS11275 0.22 -0.6 2,427,057 + AMB_RS11275 0.59 -2.1 2,427,058 - AMB_RS11275 0.59 -1.6 2,427,058 - AMB_RS11275 0.59 -2.6 2,427,198 + AMB_RS11275 0.88 -0.3 2,427,199 - AMB_RS11275 0.88 +0.3 2,427,314 + +0.6 2,427,315 - -0.2 2,427,350 + -1.0 2,427,351 - -1.5
Or see this region's nucleotide sequence