Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11185 and AMB_RS11190 overlap by 1 nucleotides AMB_RS11190 and AMB_RS11195 are separated by 27 nucleotides AMB_RS11195 and AMB_RS11200 are separated by 18 nucleotides
AMB_RS11185: AMB_RS11185 - nitrite/sulfite reductase, at 2,406,579 to 2,408,660
_RS11185
AMB_RS11190: AMB_RS11190 - phosphoadenylyl-sulfate reductase, at 2,408,660 to 2,409,358
_RS11190
AMB_RS11195: AMB_RS11195 - sulfate adenylyltransferase subunit 2, at 2,409,386 to 2,410,186
_RS11195
AMB_RS11200: AMB_RS11200 - adenylyl-sulfate kinase, at 2,410,205 to 2,412,130
_RS11200
Position (kb)
2408
2409
2410 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2407.712 kb on + strand, within AMB_RS11185 at 2407.712 kb on + strand, within AMB_RS11185 at 2407.712 kb on + strand, within AMB_RS11185 at 2407.713 kb on - strand, within AMB_RS11185 at 2407.959 kb on + strand, within AMB_RS11185 at 2407.960 kb on - strand, within AMB_RS11185 at 2407.960 kb on - strand, within AMB_RS11185 at 2407.987 kb on - strand, within AMB_RS11185 at 2408.265 kb on + strand, within AMB_RS11185 at 2408.265 kb on + strand, within AMB_RS11185 at 2408.265 kb on + strand, within AMB_RS11185 at 2408.265 kb on + strand, within AMB_RS11185 at 2408.265 kb on + strand, within AMB_RS11185 at 2408.266 kb on - strand, within AMB_RS11185 at 2408.266 kb on - strand, within AMB_RS11185 at 2408.266 kb on - strand, within AMB_RS11185 at 2408.268 kb on + strand, within AMB_RS11185 at 2408.268 kb on + strand, within AMB_RS11185 at 2408.268 kb on + strand, within AMB_RS11185 at 2408.268 kb on + strand, within AMB_RS11185 at 2408.269 kb on - strand, within AMB_RS11185 at 2408.269 kb on - strand, within AMB_RS11185 at 2408.269 kb on - strand, within AMB_RS11185 at 2408.269 kb on - strand, within AMB_RS11185 at 2408.367 kb on + strand, within AMB_RS11185 at 2408.368 kb on - strand, within AMB_RS11185 at 2408.544 kb on + strand at 2408.544 kb on + strand at 2408.545 kb on - strand at 2408.545 kb on - strand at 2408.562 kb on + strand at 2408.563 kb on - strand at 2408.563 kb on - strand at 2408.563 kb on - strand at 2408.694 kb on - strand at 2408.893 kb on - strand, within AMB_RS11190 at 2408.893 kb on - strand, within AMB_RS11190 at 2408.893 kb on - strand, within AMB_RS11190 at 2409.373 kb on + strand at 2409.373 kb on + strand at 2409.373 kb on + strand at 2409.373 kb on + strand at 2409.374 kb on - strand at 2409.422 kb on + strand at 2409.953 kb on + strand, within AMB_RS11195 at 2409.989 kb on + strand, within AMB_RS11195 at 2410.228 kb on + strand at 2410.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,407,712 + AMB_RS11185 0.54 -0.3 2,407,712 + AMB_RS11185 0.54 -1.5 2,407,712 + AMB_RS11185 0.54 -2.4 2,407,713 - AMB_RS11185 0.54 +0.3 2,407,959 + AMB_RS11185 0.66 -0.7 2,407,960 - AMB_RS11185 0.66 +0.5 2,407,960 - AMB_RS11185 0.66 -0.1 2,407,987 - AMB_RS11185 0.68 -1.8 2,408,265 + AMB_RS11185 0.81 -0.7 2,408,265 + AMB_RS11185 0.81 +1.3 2,408,265 + AMB_RS11185 0.81 -0.7 2,408,265 + AMB_RS11185 0.81 -1.0 2,408,265 + AMB_RS11185 0.81 +0.5 2,408,266 - AMB_RS11185 0.81 -0.0 2,408,266 - AMB_RS11185 0.81 +0.3 2,408,266 - AMB_RS11185 0.81 -2.9 2,408,268 + AMB_RS11185 0.81 -0.1 2,408,268 + AMB_RS11185 0.81 -0.3 2,408,268 + AMB_RS11185 0.81 +0.7 2,408,268 + AMB_RS11185 0.81 -0.4 2,408,269 - AMB_RS11185 0.81 -0.0 2,408,269 - AMB_RS11185 0.81 +0.7 2,408,269 - AMB_RS11185 0.81 +0.2 2,408,269 - AMB_RS11185 0.81 +1.3 2,408,367 + AMB_RS11185 0.86 +0.9 2,408,368 - AMB_RS11185 0.86 -1.1 2,408,544 + -2.0 2,408,544 + -0.7 2,408,545 - -1.7 2,408,545 - -0.3 2,408,562 + -2.5 2,408,563 - +0.2 2,408,563 - -1.3 2,408,563 - -0.2 2,408,694 - -0.3 2,408,893 - AMB_RS11190 0.33 -0.8 2,408,893 - AMB_RS11190 0.33 -1.8 2,408,893 - AMB_RS11190 0.33 -0.6 2,409,373 + +0.2 2,409,373 + -0.7 2,409,373 + +0.1 2,409,373 + +1.3 2,409,374 - +0.1 2,409,422 + -3.2 2,409,953 + AMB_RS11195 0.71 -0.7 2,409,989 + AMB_RS11195 0.75 -4.2 2,410,228 + +0.4 2,410,228 + +0.5
Or see this region's nucleotide sequence