Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11130 and AMB_RS11135 overlap by 7 nucleotides AMB_RS11135 and AMB_RS11140 are separated by 111 nucleotides
AMB_RS11130: AMB_RS11130 - murein transglycosylase, at 2,395,605 to 2,396,444
_RS11130
AMB_RS11135: AMB_RS11135 - DedA family protein, at 2,396,438 to 2,397,046
_RS11135
AMB_RS11140: AMB_RS11140 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 2,397,158 to 2,398,267
_RS11140
Position (kb)
2396
2397
2398 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2395.487 kb on - strand at 2395.667 kb on - strand at 2395.738 kb on + strand, within AMB_RS11130 at 2395.739 kb on - strand, within AMB_RS11130 at 2395.739 kb on - strand, within AMB_RS11130 at 2395.739 kb on - strand, within AMB_RS11130 at 2395.739 kb on - strand, within AMB_RS11130 at 2395.739 kb on - strand, within AMB_RS11130 at 2395.773 kb on + strand, within AMB_RS11130 at 2395.808 kb on + strand, within AMB_RS11130 at 2395.828 kb on - strand, within AMB_RS11130 at 2395.851 kb on - strand, within AMB_RS11130 at 2395.851 kb on - strand, within AMB_RS11130 at 2395.894 kb on - strand, within AMB_RS11130 at 2396.052 kb on + strand, within AMB_RS11130 at 2396.115 kb on + strand, within AMB_RS11130 at 2396.115 kb on + strand, within AMB_RS11130 at 2396.115 kb on + strand, within AMB_RS11130 at 2396.115 kb on + strand, within AMB_RS11130 at 2396.116 kb on - strand, within AMB_RS11130 at 2396.116 kb on - strand, within AMB_RS11130 at 2396.116 kb on - strand, within AMB_RS11130 at 2396.116 kb on - strand, within AMB_RS11130 at 2396.116 kb on - strand, within AMB_RS11130 at 2396.118 kb on + strand, within AMB_RS11130 at 2396.118 kb on + strand, within AMB_RS11130 at 2396.119 kb on - strand, within AMB_RS11130 at 2396.151 kb on + strand, within AMB_RS11130 at 2396.355 kb on + strand, within AMB_RS11130 at 2396.355 kb on + strand, within AMB_RS11130 at 2396.379 kb on + strand at 2396.379 kb on + strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.071 kb on - strand at 2397.071 kb on - strand at 2397.071 kb on - strand at 2397.097 kb on - strand at 2397.131 kb on - strand at 2397.151 kb on - strand at 2397.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,395,487 - -0.2 2,395,667 - +0.7 2,395,738 + AMB_RS11130 0.16 -0.0 2,395,739 - AMB_RS11130 0.16 -0.3 2,395,739 - AMB_RS11130 0.16 +0.1 2,395,739 - AMB_RS11130 0.16 +0.3 2,395,739 - AMB_RS11130 0.16 -0.3 2,395,739 - AMB_RS11130 0.16 -0.3 2,395,773 + AMB_RS11130 0.20 +0.2 2,395,808 + AMB_RS11130 0.24 -2.5 2,395,828 - AMB_RS11130 0.27 -1.3 2,395,851 - AMB_RS11130 0.29 -1.0 2,395,851 - AMB_RS11130 0.29 -0.4 2,395,894 - AMB_RS11130 0.34 -3.7 2,396,052 + AMB_RS11130 0.53 -1.8 2,396,115 + AMB_RS11130 0.61 -1.8 2,396,115 + AMB_RS11130 0.61 -0.9 2,396,115 + AMB_RS11130 0.61 -1.5 2,396,115 + AMB_RS11130 0.61 -1.0 2,396,116 - AMB_RS11130 0.61 +0.8 2,396,116 - AMB_RS11130 0.61 +0.2 2,396,116 - AMB_RS11130 0.61 +0.3 2,396,116 - AMB_RS11130 0.61 +1.2 2,396,116 - AMB_RS11130 0.61 -0.3 2,396,118 + AMB_RS11130 0.61 -2.8 2,396,118 + AMB_RS11130 0.61 +0.0 2,396,119 - AMB_RS11130 0.61 +0.2 2,396,151 + AMB_RS11130 0.65 -2.1 2,396,355 + AMB_RS11130 0.89 +0.1 2,396,355 + AMB_RS11130 0.89 +0.3 2,396,379 + -0.3 2,396,379 + +0.3 2,397,064 - -0.7 2,397,064 - +0.5 2,397,064 - -0.1 2,397,064 - -1.5 2,397,064 - +0.3 2,397,071 - -1.8 2,397,071 - -2.3 2,397,071 - +0.4 2,397,097 - -1.7 2,397,131 - -0.6 2,397,151 - +0.3 2,397,151 - -2.1
Or see this region's nucleotide sequence