Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS24475 and AMB_RS11015 are separated by 13 nucleotides AMB_RS11015 and AMB_RS24480 overlap by 86 nucleotides AMB_RS24480 and AMB_RS11020 overlap by 11 nucleotides AMB_RS11020 and AMB_RS11025 are separated by 59 nucleotides
AMB_RS24475: AMB_RS24475 - hypothetical protein, at 2,362,315 to 2,363,655
_RS24475
AMB_RS11015: AMB_RS11015 - XRE family transcriptional regulator, at 2,363,669 to 2,364,274
_RS11015
AMB_RS24480: AMB_RS24480 - transcriptional regulator, at 2,364,189 to 2,364,686
_RS24480
AMB_RS11020: AMB_RS11020 - HU family DNA-binding protein, at 2,364,676 to 2,364,954
_RS11020
AMB_RS11025: AMB_RS11025 - PAS domain S-box protein, at 2,365,014 to 2,366,111
_RS11025
Position (kb)
2363
2364
2365 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2362.859 kb on + strand, within AMB_RS24475 at 2362.860 kb on - strand, within AMB_RS24475 at 2362.954 kb on + strand, within AMB_RS24475 at 2363.107 kb on - strand, within AMB_RS24475 at 2363.141 kb on - strand, within AMB_RS24475 at 2363.153 kb on - strand, within AMB_RS24475 at 2363.421 kb on - strand, within AMB_RS24475 at 2363.478 kb on + strand, within AMB_RS24475 at 2363.478 kb on + strand, within AMB_RS24475 at 2363.479 kb on - strand, within AMB_RS24475 at 2363.667 kb on + strand at 2363.671 kb on - strand at 2363.671 kb on - strand at 2363.910 kb on - strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.164 kb on - strand, within AMB_RS11015 at 2364.190 kb on + strand at 2364.223 kb on + strand at 2364.355 kb on + strand, within AMB_RS24480 at 2364.355 kb on + strand, within AMB_RS24480 at 2364.356 kb on - strand, within AMB_RS24480 at 2364.356 kb on - strand, within AMB_RS24480 at 2364.356 kb on - strand, within AMB_RS24480 at 2364.414 kb on + strand, within AMB_RS24480 at 2364.414 kb on + strand, within AMB_RS24480 at 2364.415 kb on - strand, within AMB_RS24480 at 2364.421 kb on - strand, within AMB_RS24480 at 2364.421 kb on - strand, within AMB_RS24480 at 2364.513 kb on - strand, within AMB_RS24480 at 2364.584 kb on - strand, within AMB_RS24480 at 2364.640 kb on + strand at 2364.640 kb on + strand at 2364.641 kb on - strand at 2364.829 kb on + strand, within AMB_RS11020 at 2364.829 kb on + strand, within AMB_RS11020 at 2364.829 kb on + strand, within AMB_RS11020 at 2364.829 kb on + strand, within AMB_RS11020 at 2364.829 kb on + strand, within AMB_RS11020 at 2364.829 kb on + strand, within AMB_RS11020 at 2364.830 kb on - strand, within AMB_RS11020 at 2364.830 kb on - strand, within AMB_RS11020 at 2364.830 kb on - strand, within AMB_RS11020 at 2364.830 kb on - strand, within AMB_RS11020 at 2364.830 kb on - strand, within AMB_RS11020 at 2364.830 kb on - strand, within AMB_RS11020 at 2364.988 kb on + strand at 2364.989 kb on - strand at 2365.002 kb on + strand at 2365.002 kb on + strand at 2365.003 kb on - strand at 2365.003 kb on - strand at 2365.035 kb on - strand at 2365.035 kb on - strand at 2365.142 kb on - strand, within AMB_RS11025 at 2365.144 kb on - strand, within AMB_RS11025 at 2365.217 kb on + strand, within AMB_RS11025 at 2365.242 kb on - strand, within AMB_RS11025 at 2365.242 kb on - strand, within AMB_RS11025
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,362,859 + AMB_RS24475 0.41 -2.3 2,362,860 - AMB_RS24475 0.41 -1.2 2,362,954 + AMB_RS24475 0.48 -1.7 2,363,107 - AMB_RS24475 0.59 -1.1 2,363,141 - AMB_RS24475 0.62 -0.5 2,363,153 - AMB_RS24475 0.62 -0.4 2,363,421 - AMB_RS24475 0.82 -0.2 2,363,478 + AMB_RS24475 0.87 +0.4 2,363,478 + AMB_RS24475 0.87 -0.7 2,363,479 - AMB_RS24475 0.87 -1.7 2,363,667 + -1.8 2,363,671 - -0.6 2,363,671 - -0.5 2,363,910 - AMB_RS11015 0.40 +0.2 2,364,163 + AMB_RS11015 0.82 +0.5 2,364,163 + AMB_RS11015 0.82 +0.5 2,364,163 + AMB_RS11015 0.82 -0.0 2,364,163 + AMB_RS11015 0.82 -3.6 2,364,163 + AMB_RS11015 0.82 +0.6 2,364,164 - AMB_RS11015 0.82 -1.5 2,364,190 + +0.2 2,364,223 + -0.0 2,364,355 + AMB_RS24480 0.33 +0.0 2,364,355 + AMB_RS24480 0.33 -0.0 2,364,356 - AMB_RS24480 0.34 -1.5 2,364,356 - AMB_RS24480 0.34 +1.5 2,364,356 - AMB_RS24480 0.34 -0.5 2,364,414 + AMB_RS24480 0.45 -0.3 2,364,414 + AMB_RS24480 0.45 +2.3 2,364,415 - AMB_RS24480 0.45 -0.7 2,364,421 - AMB_RS24480 0.47 +0.3 2,364,421 - AMB_RS24480 0.47 -0.5 2,364,513 - AMB_RS24480 0.65 -0.2 2,364,584 - AMB_RS24480 0.79 -0.1 2,364,640 + -1.3 2,364,640 + -1.5 2,364,641 - -0.1 2,364,829 + AMB_RS11020 0.55 +0.3 2,364,829 + AMB_RS11020 0.55 -1.0 2,364,829 + AMB_RS11020 0.55 +0.5 2,364,829 + AMB_RS11020 0.55 -1.0 2,364,829 + AMB_RS11020 0.55 -0.7 2,364,829 + AMB_RS11020 0.55 +0.4 2,364,830 - AMB_RS11020 0.55 +0.1 2,364,830 - AMB_RS11020 0.55 -1.3 2,364,830 - AMB_RS11020 0.55 -1.0 2,364,830 - AMB_RS11020 0.55 -0.3 2,364,830 - AMB_RS11020 0.55 +0.1 2,364,830 - AMB_RS11020 0.55 -0.3 2,364,988 + +0.6 2,364,989 - -0.0 2,365,002 + -0.5 2,365,002 + -2.1 2,365,003 - -1.5 2,365,003 - -1.0 2,365,035 - -0.3 2,365,035 - +0.5 2,365,142 - AMB_RS11025 0.12 -1.3 2,365,144 - AMB_RS11025 0.12 -0.3 2,365,217 + AMB_RS11025 0.18 +0.4 2,365,242 - AMB_RS11025 0.21 -3.3 2,365,242 - AMB_RS11025 0.21 +0.3
Or see this region's nucleotide sequence