Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10730 and AMB_RS10735 are separated by 114 nucleotides
AMB_RS10730: AMB_RS10730 - hypothetical protein, at 2,299,953 to 2,300,216
_RS10730
AMB_RS10735: AMB_RS10735 - integrase, at 2,300,331 to 2,301,548
_RS10735
Position (kb)
2299
2300
2301 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2299.345 kb on - strand at 2299.348 kb on + strand at 2299.348 kb on + strand at 2299.417 kb on + strand at 2299.476 kb on - strand at 2299.567 kb on + strand at 2299.568 kb on - strand at 2299.713 kb on + strand at 2299.713 kb on + strand at 2300.110 kb on + strand, within AMB_RS10730 at 2300.110 kb on + strand, within AMB_RS10730 at 2300.200 kb on - strand at 2300.223 kb on - strand at 2300.223 kb on - strand at 2300.227 kb on - strand at 2300.227 kb on - strand at 2300.227 kb on - strand at 2300.305 kb on - strand at 2300.313 kb on + strand at 2300.313 kb on + strand at 2300.314 kb on - strand at 2300.314 kb on - strand at 2300.314 kb on - strand at 2300.314 kb on - strand at 2300.364 kb on + strand at 2300.364 kb on + strand at 2300.364 kb on + strand at 2300.364 kb on + strand at 2300.364 kb on + strand at 2300.364 kb on + strand at 2300.364 kb on + strand at 2300.365 kb on - strand at 2300.365 kb on - strand at 2300.365 kb on - strand at 2300.365 kb on - strand at 2300.365 kb on - strand at 2300.365 kb on - strand at 2300.365 kb on - strand at 2300.375 kb on - strand at 2300.425 kb on + strand at 2300.425 kb on + strand at 2300.464 kb on + strand, within AMB_RS10735 at 2300.465 kb on - strand, within AMB_RS10735 at 2300.480 kb on + strand, within AMB_RS10735 at 2300.483 kb on - strand, within AMB_RS10735 at 2300.483 kb on - strand, within AMB_RS10735 at 2300.565 kb on + strand, within AMB_RS10735 at 2300.565 kb on + strand, within AMB_RS10735 at 2300.565 kb on + strand, within AMB_RS10735 at 2300.582 kb on + strand, within AMB_RS10735 at 2300.638 kb on + strand, within AMB_RS10735 at 2300.639 kb on - strand, within AMB_RS10735 at 2300.760 kb on + strand, within AMB_RS10735 at 2300.760 kb on + strand, within AMB_RS10735 at 2300.760 kb on + strand, within AMB_RS10735 at 2300.760 kb on + strand, within AMB_RS10735 at 2300.761 kb on - strand, within AMB_RS10735 at 2300.761 kb on - strand, within AMB_RS10735 at 2300.761 kb on - strand, within AMB_RS10735 at 2300.837 kb on - strand, within AMB_RS10735 at 2300.910 kb on + strand, within AMB_RS10735 at 2300.911 kb on - strand, within AMB_RS10735 at 2300.911 kb on - strand, within AMB_RS10735 at 2300.923 kb on + strand, within AMB_RS10735 at 2300.923 kb on + strand, within AMB_RS10735 at 2301.090 kb on + strand, within AMB_RS10735 at 2301.090 kb on + strand, within AMB_RS10735 at 2301.090 kb on + strand, within AMB_RS10735 at 2301.090 kb on + strand, within AMB_RS10735 at 2301.091 kb on - strand, within AMB_RS10735 at 2301.091 kb on - strand, within AMB_RS10735 at 2301.091 kb on - strand, within AMB_RS10735 at 2301.145 kb on + strand, within AMB_RS10735 at 2301.209 kb on - strand, within AMB_RS10735 at 2301.216 kb on + strand, within AMB_RS10735
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,299,345 - -1.2 2,299,348 + -2.8 2,299,348 + -0.4 2,299,417 + -0.4 2,299,476 - -0.8 2,299,567 + -2.3 2,299,568 - -1.1 2,299,713 + +0.3 2,299,713 + -0.3 2,300,110 + AMB_RS10730 0.59 -1.7 2,300,110 + AMB_RS10730 0.59 -0.0 2,300,200 - -0.3 2,300,223 - -0.3 2,300,223 - -1.4 2,300,227 - -1.5 2,300,227 - +0.4 2,300,227 - -0.5 2,300,305 - -0.4 2,300,313 + +0.5 2,300,313 + -0.3 2,300,314 - +0.8 2,300,314 - -0.2 2,300,314 - -1.3 2,300,314 - -1.3 2,300,364 + -0.3 2,300,364 + -3.6 2,300,364 + +0.4 2,300,364 + -1.5 2,300,364 + -0.1 2,300,364 + -0.3 2,300,364 + -0.4 2,300,365 - +0.3 2,300,365 - -2.4 2,300,365 - +0.2 2,300,365 - +0.1 2,300,365 - +0.3 2,300,365 - -1.3 2,300,365 - -0.6 2,300,375 - -3.4 2,300,425 + +0.3 2,300,425 + +0.5 2,300,464 + AMB_RS10735 0.11 -0.2 2,300,465 - AMB_RS10735 0.11 -1.0 2,300,480 + AMB_RS10735 0.12 +0.2 2,300,483 - AMB_RS10735 0.12 -0.1 2,300,483 - AMB_RS10735 0.12 -0.4 2,300,565 + AMB_RS10735 0.19 -0.3 2,300,565 + AMB_RS10735 0.19 -2.8 2,300,565 + AMB_RS10735 0.19 -0.1 2,300,582 + AMB_RS10735 0.21 -0.6 2,300,638 + AMB_RS10735 0.25 +0.2 2,300,639 - AMB_RS10735 0.25 +0.5 2,300,760 + AMB_RS10735 0.35 -0.0 2,300,760 + AMB_RS10735 0.35 -0.2 2,300,760 + AMB_RS10735 0.35 -0.2 2,300,760 + AMB_RS10735 0.35 +0.3 2,300,761 - AMB_RS10735 0.35 -3.8 2,300,761 - AMB_RS10735 0.35 +0.2 2,300,761 - AMB_RS10735 0.35 -0.3 2,300,837 - AMB_RS10735 0.42 -0.3 2,300,910 + AMB_RS10735 0.48 +0.6 2,300,911 - AMB_RS10735 0.48 +0.4 2,300,911 - AMB_RS10735 0.48 +1.1 2,300,923 + AMB_RS10735 0.49 -0.3 2,300,923 + AMB_RS10735 0.49 -3.0 2,301,090 + AMB_RS10735 0.62 +0.5 2,301,090 + AMB_RS10735 0.62 -0.0 2,301,090 + AMB_RS10735 0.62 -2.0 2,301,090 + AMB_RS10735 0.62 -1.0 2,301,091 - AMB_RS10735 0.62 -1.0 2,301,091 - AMB_RS10735 0.62 +0.1 2,301,091 - AMB_RS10735 0.62 +1.3 2,301,145 + AMB_RS10735 0.67 -2.0 2,301,209 - AMB_RS10735 0.72 +0.1 2,301,216 + AMB_RS10735 0.73 +0.0
Or see this region's nucleotide sequence