Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10225 and AMB_RS10230 overlap by 38 nucleotides AMB_RS10230 and AMB_RS10235 overlap by 1 nucleotides AMB_RS10235 and AMB_RS10240 overlap by 4 nucleotides
AMB_RS10225: AMB_RS10225 - feruloyl-CoA synthase, at 2,202,166 to 2,204,100
_RS10225
AMB_RS10230: AMB_RS10230 - ABC transporter ATP-binding protein, at 2,204,063 to 2,204,767
_RS10230
AMB_RS10235: AMB_RS10235 - ABC transporter ATP-binding protein, at 2,204,767 to 2,205,504
_RS10235
AMB_RS10240: AMB_RS10240 - branched-chain amino acid ABC transporter permease, at 2,205,501 to 2,206,490
_RS10240
Position (kb)
2204
2205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2203.091 kb on + strand, within AMB_RS10225 at 2203.092 kb on - strand, within AMB_RS10225 at 2203.092 kb on - strand, within AMB_RS10225 at 2203.092 kb on - strand, within AMB_RS10225 at 2203.170 kb on - strand, within AMB_RS10225 at 2203.556 kb on + strand, within AMB_RS10225 at 2203.556 kb on + strand, within AMB_RS10225 at 2203.557 kb on - strand, within AMB_RS10225 at 2203.589 kb on + strand, within AMB_RS10225 at 2203.589 kb on + strand, within AMB_RS10225 at 2203.589 kb on + strand, within AMB_RS10225 at 2203.589 kb on + strand, within AMB_RS10225 at 2203.589 kb on + strand, within AMB_RS10225 at 2203.589 kb on + strand, within AMB_RS10225 at 2203.590 kb on - strand, within AMB_RS10225 at 2203.625 kb on + strand, within AMB_RS10225 at 2203.625 kb on + strand, within AMB_RS10225 at 2203.626 kb on - strand, within AMB_RS10225 at 2203.895 kb on + strand, within AMB_RS10225 at 2203.896 kb on - strand, within AMB_RS10225 at 2204.005 kb on - strand at 2204.064 kb on + strand at 2204.064 kb on + strand at 2204.064 kb on + strand at 2204.065 kb on - strand at 2204.065 kb on - strand at 2204.065 kb on - strand at 2204.076 kb on + strand at 2204.076 kb on + strand at 2204.076 kb on + strand at 2204.077 kb on - strand at 2204.077 kb on - strand at 2204.077 kb on - strand at 2204.154 kb on + strand, within AMB_RS10230 at 2204.154 kb on + strand, within AMB_RS10230 at 2204.155 kb on - strand, within AMB_RS10230 at 2204.155 kb on - strand, within AMB_RS10230 at 2204.415 kb on + strand, within AMB_RS10230 at 2204.415 kb on + strand, within AMB_RS10230 at 2204.415 kb on + strand, within AMB_RS10230 at 2204.789 kb on + strand at 2204.790 kb on - strand at 2204.790 kb on - strand at 2204.790 kb on - strand at 2204.790 kb on - strand at 2204.790 kb on - strand at 2204.790 kb on - strand at 2204.817 kb on + strand at 2204.818 kb on - strand at 2205.499 kb on - strand at 2205.575 kb on + strand at 2205.575 kb on + strand at 2205.576 kb on - strand at 2205.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,203,091 + AMB_RS10225 0.48 -2.0 2,203,092 - AMB_RS10225 0.48 +0.2 2,203,092 - AMB_RS10225 0.48 -1.0 2,203,092 - AMB_RS10225 0.48 -0.5 2,203,170 - AMB_RS10225 0.52 -0.7 2,203,556 + AMB_RS10225 0.72 -0.4 2,203,556 + AMB_RS10225 0.72 -1.0 2,203,557 - AMB_RS10225 0.72 -0.6 2,203,589 + AMB_RS10225 0.74 -0.3 2,203,589 + AMB_RS10225 0.74 -1.5 2,203,589 + AMB_RS10225 0.74 -1.3 2,203,589 + AMB_RS10225 0.74 -1.9 2,203,589 + AMB_RS10225 0.74 -1.0 2,203,589 + AMB_RS10225 0.74 -3.1 2,203,590 - AMB_RS10225 0.74 -2.0 2,203,625 + AMB_RS10225 0.75 -0.5 2,203,625 + AMB_RS10225 0.75 -1.5 2,203,626 - AMB_RS10225 0.75 -1.7 2,203,895 + AMB_RS10225 0.89 -0.7 2,203,896 - AMB_RS10225 0.89 +0.0 2,204,005 - -0.8 2,204,064 + +0.3 2,204,064 + +0.3 2,204,064 + -1.3 2,204,065 - -0.3 2,204,065 - -0.3 2,204,065 - +0.3 2,204,076 + -3.0 2,204,076 + -1.5 2,204,076 + -1.0 2,204,077 - -1.3 2,204,077 - -1.0 2,204,077 - +0.5 2,204,154 + AMB_RS10230 0.13 +0.4 2,204,154 + AMB_RS10230 0.13 -0.7 2,204,155 - AMB_RS10230 0.13 -0.3 2,204,155 - AMB_RS10230 0.13 -1.9 2,204,415 + AMB_RS10230 0.50 +1.2 2,204,415 + AMB_RS10230 0.50 -2.4 2,204,415 + AMB_RS10230 0.50 -1.0 2,204,789 + +0.4 2,204,790 - -2.2 2,204,790 - -0.8 2,204,790 - -0.1 2,204,790 - +2.2 2,204,790 - -0.3 2,204,790 - +1.3 2,204,817 + +0.3 2,204,818 - -0.7 2,205,499 - +0.5 2,205,575 + -0.1 2,205,575 + +0.3 2,205,576 - -0.1 2,205,596 - +0.3
Or see this region's nucleotide sequence