Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09390 and AMB_RS09395 are separated by 10 nucleotides AMB_RS09395 and AMB_RS09400 overlap by 4 nucleotides AMB_RS09400 and AMB_RS09405 overlap by 1 nucleotides
AMB_RS09390: AMB_RS09390 - Crp/Fnr family transcriptional regulator, at 1,998,561 to 1,999,289
_RS09390
AMB_RS09395: AMB_RS09395 - ABC transporter ATP-binding protein, at 1,999,300 to 2,000,121
_RS09395
AMB_RS09400: AMB_RS09400 - ABC transporter permease, at 2,000,118 to 2,001,002
_RS09400
AMB_RS09405: AMB_RS09405 - ABC transporter substrate-binding protein, at 2,001,002 to 2,002,411
_RS09405
Position (kb)
2000
2001
2002 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1999.180 kb on + strand, within AMB_RS09390 at 1999.181 kb on - strand, within AMB_RS09390 at 1999.742 kb on + strand, within AMB_RS09395 at 1999.742 kb on + strand, within AMB_RS09395 at 1999.742 kb on + strand, within AMB_RS09395 at 1999.743 kb on - strand, within AMB_RS09395 at 2000.269 kb on + strand, within AMB_RS09400 at 2000.269 kb on + strand, within AMB_RS09400 at 2000.269 kb on + strand, within AMB_RS09400 at 2000.269 kb on + strand, within AMB_RS09400 at 2000.269 kb on + strand, within AMB_RS09400 at 2000.270 kb on - strand, within AMB_RS09400 at 2000.537 kb on + strand, within AMB_RS09400 at 2000.537 kb on + strand, within AMB_RS09400 at 2000.623 kb on + strand, within AMB_RS09400 at 2000.963 kb on + strand at 2001.135 kb on + strand at 2001.136 kb on - strand at 2001.136 kb on - strand at 2001.136 kb on - strand at 2001.136 kb on - strand at 2001.180 kb on + strand, within AMB_RS09405 at 2001.186 kb on + strand, within AMB_RS09405 at 2001.186 kb on + strand, within AMB_RS09405 at 2001.186 kb on + strand, within AMB_RS09405 at 2001.187 kb on - strand, within AMB_RS09405 at 2001.187 kb on - strand, within AMB_RS09405 at 2001.187 kb on - strand, within AMB_RS09405 at 2001.187 kb on - strand, within AMB_RS09405 at 2001.191 kb on + strand, within AMB_RS09405 at 2001.191 kb on + strand, within AMB_RS09405 at 2001.191 kb on + strand, within AMB_RS09405 at 2001.192 kb on - strand, within AMB_RS09405 at 2001.192 kb on - strand, within AMB_RS09405 at 2001.258 kb on + strand, within AMB_RS09405 at 2001.354 kb on + strand, within AMB_RS09405 at 2001.354 kb on + strand, within AMB_RS09405 at 2001.354 kb on + strand, within AMB_RS09405 at 2001.384 kb on + strand, within AMB_RS09405 at 2001.406 kb on - strand, within AMB_RS09405 at 2001.406 kb on - strand, within AMB_RS09405 at 2001.420 kb on + strand, within AMB_RS09405 at 2001.421 kb on - strand, within AMB_RS09405 at 2001.421 kb on - strand, within AMB_RS09405 at 2001.421 kb on - strand, within AMB_RS09405 at 2001.567 kb on + strand, within AMB_RS09405 at 2001.567 kb on + strand, within AMB_RS09405 at 2001.568 kb on - strand, within AMB_RS09405 at 2001.568 kb on - strand, within AMB_RS09405 at 2001.568 kb on - strand, within AMB_RS09405 at 2001.669 kb on + strand, within AMB_RS09405 at 2001.691 kb on - strand, within AMB_RS09405 at 2001.691 kb on - strand, within AMB_RS09405 at 2001.760 kb on - strand, within AMB_RS09405 at 2001.760 kb on - strand, within AMB_RS09405 at 2001.760 kb on - strand, within AMB_RS09405 at 2001.760 kb on - strand, within AMB_RS09405 at 2001.888 kb on + strand, within AMB_RS09405 at 2001.993 kb on + strand, within AMB_RS09405 at 2001.993 kb on + strand, within AMB_RS09405 at 2001.994 kb on - strand, within AMB_RS09405 at 2001.994 kb on - strand, within AMB_RS09405 at 2001.994 kb on - strand, within AMB_RS09405 at 2001.994 kb on - strand, within AMB_RS09405 at 2001.996 kb on + strand, within AMB_RS09405 at 2001.997 kb on - strand, within AMB_RS09405
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,999,180 + AMB_RS09390 0.85 +0.1 1,999,181 - AMB_RS09390 0.85 +0.5 1,999,742 + AMB_RS09395 0.54 -1.0 1,999,742 + AMB_RS09395 0.54 -1.5 1,999,742 + AMB_RS09395 0.54 +0.2 1,999,743 - AMB_RS09395 0.54 -1.5 2,000,269 + AMB_RS09400 0.17 -0.1 2,000,269 + AMB_RS09400 0.17 -2.8 2,000,269 + AMB_RS09400 0.17 +0.1 2,000,269 + AMB_RS09400 0.17 -1.5 2,000,269 + AMB_RS09400 0.17 +0.6 2,000,270 - AMB_RS09400 0.17 -0.0 2,000,537 + AMB_RS09400 0.47 -0.1 2,000,537 + AMB_RS09400 0.47 -1.2 2,000,623 + AMB_RS09400 0.57 -0.2 2,000,963 + +0.4 2,001,135 + -1.5 2,001,136 - -0.2 2,001,136 - +0.8 2,001,136 - +0.1 2,001,136 - +0.4 2,001,180 + AMB_RS09405 0.13 +1.2 2,001,186 + AMB_RS09405 0.13 +0.0 2,001,186 + AMB_RS09405 0.13 -1.5 2,001,186 + AMB_RS09405 0.13 -0.7 2,001,187 - AMB_RS09405 0.13 -1.7 2,001,187 - AMB_RS09405 0.13 +0.2 2,001,187 - AMB_RS09405 0.13 -0.7 2,001,187 - AMB_RS09405 0.13 -0.7 2,001,191 + AMB_RS09405 0.13 -0.3 2,001,191 + AMB_RS09405 0.13 -1.2 2,001,191 + AMB_RS09405 0.13 +0.3 2,001,192 - AMB_RS09405 0.13 -3.1 2,001,192 - AMB_RS09405 0.13 -0.2 2,001,258 + AMB_RS09405 0.18 -1.0 2,001,354 + AMB_RS09405 0.25 -1.0 2,001,354 + AMB_RS09405 0.25 -1.2 2,001,354 + AMB_RS09405 0.25 -1.5 2,001,384 + AMB_RS09405 0.27 -0.5 2,001,406 - AMB_RS09405 0.29 -1.2 2,001,406 - AMB_RS09405 0.29 +0.0 2,001,420 + AMB_RS09405 0.30 +0.5 2,001,421 - AMB_RS09405 0.30 +0.2 2,001,421 - AMB_RS09405 0.30 -1.3 2,001,421 - AMB_RS09405 0.30 +1.0 2,001,567 + AMB_RS09405 0.40 -0.1 2,001,567 + AMB_RS09405 0.40 -2.1 2,001,568 - AMB_RS09405 0.40 -0.1 2,001,568 - AMB_RS09405 0.40 -2.0 2,001,568 - AMB_RS09405 0.40 +0.7 2,001,669 + AMB_RS09405 0.47 +0.1 2,001,691 - AMB_RS09405 0.49 -0.2 2,001,691 - AMB_RS09405 0.49 -1.5 2,001,760 - AMB_RS09405 0.54 -0.3 2,001,760 - AMB_RS09405 0.54 -1.7 2,001,760 - AMB_RS09405 0.54 +0.6 2,001,760 - AMB_RS09405 0.54 +0.3 2,001,888 + AMB_RS09405 0.63 +0.1 2,001,993 + AMB_RS09405 0.70 -0.4 2,001,993 + AMB_RS09405 0.70 +0.3 2,001,994 - AMB_RS09405 0.70 -1.2 2,001,994 - AMB_RS09405 0.70 +0.3 2,001,994 - AMB_RS09405 0.70 -1.8 2,001,994 - AMB_RS09405 0.70 +0.0 2,001,996 + AMB_RS09405 0.70 +0.3 2,001,997 - AMB_RS09405 0.71 -1.2
Or see this region's nucleotide sequence