Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07730 and AMB_RS07735 overlap by 4 nucleotides AMB_RS07735 and AMB_RS07740 overlap by 17 nucleotides AMB_RS07740 and AMB_RS07745 overlap by 4 nucleotides AMB_RS07745 and AMB_RS07750 are separated by 113 nucleotides
AMB_RS07730: AMB_RS07730 - NUDIX hydrolase, at 1,661,143 to 1,661,859
_RS07730
AMB_RS07735: AMB_RS07735 - histidine phosphatase family protein, at 1,661,856 to 1,662,440
_RS07735
AMB_RS07740: AMB_RS07740 - demethoxyubiquinone hydroxylase family protein, at 1,662,424 to 1,662,981
_RS07740
AMB_RS07745: AMB_RS07745 - disulfide bond formation protein B, at 1,662,978 to 1,663,457
_RS07745
AMB_RS07750: AMB_RS07750 - periplasmic protein kinase ArgK, at 1,663,571 to 1,664,500
_RS07750
Position (kb)
1662
1663 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1661.437 kb on + strand, within AMB_RS07730 at 1661.437 kb on + strand, within AMB_RS07730 at 1661.438 kb on - strand, within AMB_RS07730 at 1661.438 kb on - strand, within AMB_RS07730 at 1661.442 kb on + strand, within AMB_RS07730 at 1661.580 kb on + strand, within AMB_RS07730 at 1661.581 kb on - strand, within AMB_RS07730 at 1661.686 kb on + strand, within AMB_RS07730 at 1661.686 kb on + strand, within AMB_RS07730 at 1662.199 kb on - strand, within AMB_RS07735 at 1662.620 kb on + strand, within AMB_RS07740 at 1662.780 kb on + strand, within AMB_RS07740 at 1662.872 kb on + strand, within AMB_RS07740 at 1663.213 kb on + strand, within AMB_RS07745 at 1663.213 kb on + strand, within AMB_RS07745 at 1663.213 kb on + strand, within AMB_RS07745 at 1663.213 kb on + strand, within AMB_RS07745 at 1663.213 kb on + strand, within AMB_RS07745 at 1663.375 kb on + strand, within AMB_RS07745 at 1663.375 kb on + strand, within AMB_RS07745 at 1663.451 kb on + strand at 1663.451 kb on + strand at 1663.451 kb on + strand at 1663.468 kb on + strand at 1663.468 kb on + strand at 1663.469 kb on - strand at 1663.469 kb on - strand at 1663.469 kb on - strand at 1663.469 kb on - strand at 1663.469 kb on - strand at 1663.469 kb on - strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.470 kb on + strand at 1663.471 kb on - strand at 1663.471 kb on - strand at 1663.471 kb on - strand at 1663.471 kb on - strand at 1663.471 kb on - strand at 1663.735 kb on + strand, within AMB_RS07750 at 1663.736 kb on - strand, within AMB_RS07750 at 1663.736 kb on - strand, within AMB_RS07750 at 1663.736 kb on - strand, within AMB_RS07750 at 1663.736 kb on - strand, within AMB_RS07750 at 1663.900 kb on + strand, within AMB_RS07750 at 1663.901 kb on - strand, within AMB_RS07750
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,661,437 + AMB_RS07730 0.41 +0.1 1,661,437 + AMB_RS07730 0.41 -0.4 1,661,438 - AMB_RS07730 0.41 +0.3 1,661,438 - AMB_RS07730 0.41 -1.2 1,661,442 + AMB_RS07730 0.42 -1.5 1,661,580 + AMB_RS07730 0.61 -1.7 1,661,581 - AMB_RS07730 0.61 -1.7 1,661,686 + AMB_RS07730 0.76 -2.4 1,661,686 + AMB_RS07730 0.76 -0.2 1,662,199 - AMB_RS07735 0.59 -1.7 1,662,620 + AMB_RS07740 0.35 -2.1 1,662,780 + AMB_RS07740 0.64 +0.4 1,662,872 + AMB_RS07740 0.80 -2.0 1,663,213 + AMB_RS07745 0.49 -1.2 1,663,213 + AMB_RS07745 0.49 -0.8 1,663,213 + AMB_RS07745 0.49 -3.4 1,663,213 + AMB_RS07745 0.49 -2.6 1,663,213 + AMB_RS07745 0.49 -2.4 1,663,375 + AMB_RS07745 0.83 -1.0 1,663,375 + AMB_RS07745 0.83 -0.4 1,663,451 + +1.4 1,663,451 + -2.5 1,663,451 + -3.8 1,663,468 + -3.2 1,663,468 + -2.4 1,663,469 - -0.2 1,663,469 - +0.1 1,663,469 - -0.3 1,663,469 - -0.9 1,663,469 - -1.2 1,663,469 - +1.4 1,663,470 + -1.4 1,663,470 + +0.4 1,663,470 + -3.5 1,663,470 + -2.7 1,663,470 + -3.0 1,663,470 + -2.2 1,663,470 + -2.7 1,663,470 + -2.7 1,663,470 + -1.5 1,663,471 - +1.0 1,663,471 - +0.1 1,663,471 - -2.8 1,663,471 - -0.8 1,663,471 - +2.0 1,663,735 + AMB_RS07750 0.18 +0.2 1,663,736 - AMB_RS07750 0.18 +0.2 1,663,736 - AMB_RS07750 0.18 +0.4 1,663,736 - AMB_RS07750 0.18 +1.3 1,663,736 - AMB_RS07750 0.18 -0.2 1,663,900 + AMB_RS07750 0.35 +0.5 1,663,901 - AMB_RS07750 0.35 +0.8
Or see this region's nucleotide sequence