Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07540 and AMB_RS07545 overlap by 1 nucleotides AMB_RS07545 and AMB_RS07550 are separated by 59 nucleotides AMB_RS07550 and AMB_RS07555 are separated by 45 nucleotides AMB_RS07555 and AMB_RS07560 are separated by 6 nucleotides AMB_RS07560 and AMB_RS07565 are separated by 5 nucleotides
AMB_RS07540: AMB_RS07540 - reverse transcriptase, at 1,632,862 to 1,634,274
_RS07540
AMB_RS07545: AMB_RS07545 - four helix bundle protein, at 1,634,274 to 1,634,627
_RS07545
AMB_RS07550: AMB_RS07550 - DUF1566 domain-containing protein, at 1,634,687 to 1,635,238
_RS07550
AMB_RS07555: AMB_RS07555 - DUF1566 domain-containing protein, at 1,635,284 to 1,635,856
_RS07555
AMB_RS07560: AMB_RS07560 - hypothetical protein, at 1,635,863 to 1,636,198
_RS07560
AMB_RS07565: AMB_RS07565 - single-stranded DNA-binding protein, at 1,636,204 to 1,636,785
_RS07565
Position (kb)
1634
1635
1636 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1633.730 kb on + strand, within AMB_RS07540 at 1633.730 kb on + strand, within AMB_RS07540 at 1633.934 kb on + strand, within AMB_RS07540 at 1633.934 kb on + strand, within AMB_RS07540 at 1633.937 kb on + strand, within AMB_RS07540 at 1633.937 kb on + strand, within AMB_RS07540 at 1633.937 kb on + strand, within AMB_RS07540 at 1633.937 kb on + strand, within AMB_RS07540 at 1633.937 kb on + strand, within AMB_RS07540 at 1634.098 kb on + strand, within AMB_RS07540 at 1634.176 kb on + strand at 1634.290 kb on + strand at 1634.587 kb on + strand, within AMB_RS07545 at 1634.587 kb on + strand, within AMB_RS07545 at 1634.587 kb on + strand, within AMB_RS07545 at 1634.588 kb on - strand, within AMB_RS07545 at 1634.757 kb on + strand, within AMB_RS07550 at 1634.757 kb on + strand, within AMB_RS07550 at 1634.760 kb on + strand, within AMB_RS07550 at 1634.760 kb on + strand, within AMB_RS07550 at 1634.760 kb on + strand, within AMB_RS07550 at 1634.760 kb on + strand, within AMB_RS07550 at 1634.766 kb on + strand, within AMB_RS07550 at 1634.793 kb on + strand, within AMB_RS07550 at 1634.812 kb on - strand, within AMB_RS07550 at 1635.151 kb on + strand, within AMB_RS07550 at 1635.153 kb on + strand, within AMB_RS07550 at 1635.345 kb on + strand, within AMB_RS07555 at 1635.345 kb on + strand, within AMB_RS07555 at 1635.387 kb on + strand, within AMB_RS07555 at 1635.839 kb on - strand at 1635.844 kb on + strand at 1635.844 kb on + strand at 1635.844 kb on + strand at 1635.844 kb on + strand at 1635.845 kb on - strand at 1635.845 kb on - strand at 1635.845 kb on - strand at 1635.864 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,633,730 + AMB_RS07540 0.61 -1.3 1,633,730 + AMB_RS07540 0.61 -0.3 1,633,934 + AMB_RS07540 0.76 -0.5 1,633,934 + AMB_RS07540 0.76 -0.6 1,633,937 + AMB_RS07540 0.76 +0.2 1,633,937 + AMB_RS07540 0.76 +0.1 1,633,937 + AMB_RS07540 0.76 -0.3 1,633,937 + AMB_RS07540 0.76 -1.3 1,633,937 + AMB_RS07540 0.76 +0.5 1,634,098 + AMB_RS07540 0.87 -2.4 1,634,176 + -0.3 1,634,290 + -1.0 1,634,587 + AMB_RS07545 0.88 -0.1 1,634,587 + AMB_RS07545 0.88 +0.0 1,634,587 + AMB_RS07545 0.88 -3.3 1,634,588 - AMB_RS07545 0.89 -1.5 1,634,757 + AMB_RS07550 0.13 -1.5 1,634,757 + AMB_RS07550 0.13 +0.1 1,634,760 + AMB_RS07550 0.13 -0.3 1,634,760 + AMB_RS07550 0.13 -1.5 1,634,760 + AMB_RS07550 0.13 -0.0 1,634,760 + AMB_RS07550 0.13 -0.2 1,634,766 + AMB_RS07550 0.14 -2.4 1,634,793 + AMB_RS07550 0.19 -0.0 1,634,812 - AMB_RS07550 0.23 -0.3 1,635,151 + AMB_RS07550 0.84 -0.3 1,635,153 + AMB_RS07550 0.84 -0.4 1,635,345 + AMB_RS07555 0.11 -0.5 1,635,345 + AMB_RS07555 0.11 -0.7 1,635,387 + AMB_RS07555 0.18 -1.0 1,635,839 - -1.5 1,635,844 + -2.8 1,635,844 + -1.8 1,635,844 + -0.1 1,635,844 + -1.7 1,635,845 - -4.0 1,635,845 - -0.4 1,635,845 - -1.5 1,635,864 + -1.0
Or see this region's nucleotide sequence