Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07210 and AMB_RS07215 are separated by 296 nucleotides AMB_RS07215 and AMB_RS07220 are separated by 56 nucleotides AMB_RS07220 and AMB_RS07225 are separated by 144 nucleotides
AMB_RS07210: AMB_RS07210 - permease, at 1,553,488 to 1,555,242
_RS07210
AMB_RS07215: AMB_RS07215 - ATP-dependent helicase, at 1,555,539 to 1,556,969
_RS07215
AMB_RS07220: AMB_RS07220 - NUDIX domain-containing protein, at 1,557,026 to 1,557,457
_RS07220
AMB_RS07225: AMB_RS07225 - PAS domain S-box protein, at 1,557,602 to 1,559,095
_RS07225
Position (kb)
1555
1556
1557 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1554.793 kb on + strand, within AMB_RS07210 at 1554.793 kb on + strand, within AMB_RS07210 at 1554.957 kb on + strand, within AMB_RS07210 at 1554.957 kb on + strand, within AMB_RS07210 at 1554.957 kb on + strand, within AMB_RS07210 at 1554.957 kb on + strand, within AMB_RS07210 at 1554.957 kb on + strand, within AMB_RS07210 at 1554.958 kb on - strand, within AMB_RS07210 at 1554.958 kb on - strand, within AMB_RS07210 at 1554.958 kb on - strand, within AMB_RS07210 at 1554.958 kb on - strand, within AMB_RS07210 at 1555.162 kb on + strand at 1555.162 kb on + strand at 1555.241 kb on - strand at 1555.416 kb on + strand at 1555.538 kb on + strand at 1555.538 kb on + strand at 1555.538 kb on + strand at 1555.538 kb on + strand at 1555.539 kb on - strand at 1555.539 kb on - strand at 1555.539 kb on - strand at 1555.605 kb on + strand at 1555.605 kb on + strand at 1555.606 kb on - strand at 1555.833 kb on + strand, within AMB_RS07215 at 1555.833 kb on + strand, within AMB_RS07215 at 1555.834 kb on - strand, within AMB_RS07215 at 1555.834 kb on - strand, within AMB_RS07215 at 1555.834 kb on - strand, within AMB_RS07215 at 1556.526 kb on + strand, within AMB_RS07215 at 1556.527 kb on - strand, within AMB_RS07215 at 1556.607 kb on + strand, within AMB_RS07215 at 1556.656 kb on - strand, within AMB_RS07215 at 1556.667 kb on + strand, within AMB_RS07215 at 1556.668 kb on - strand, within AMB_RS07215 at 1556.989 kb on + strand at 1556.990 kb on - strand at 1557.189 kb on + strand, within AMB_RS07220 at 1557.189 kb on + strand, within AMB_RS07220 at 1557.189 kb on + strand, within AMB_RS07220 at 1557.189 kb on + strand, within AMB_RS07220 at 1557.189 kb on + strand, within AMB_RS07220 at 1557.190 kb on - strand, within AMB_RS07220 at 1557.190 kb on - strand, within AMB_RS07220 at 1557.190 kb on - strand, within AMB_RS07220 at 1557.190 kb on - strand, within AMB_RS07220 at 1557.190 kb on - strand, within AMB_RS07220 at 1557.190 kb on - strand, within AMB_RS07220 at 1557.195 kb on + strand, within AMB_RS07220 at 1557.444 kb on + strand at 1557.444 kb on + strand at 1557.445 kb on - strand at 1557.489 kb on + strand at 1557.549 kb on + strand at 1557.550 kb on - strand at 1557.553 kb on - strand at 1557.553 kb on - strand at 1557.553 kb on - strand at 1557.565 kb on + strand at 1557.566 kb on - strand at 1557.566 kb on - strand at 1557.566 kb on - strand at 1557.572 kb on - strand at 1557.573 kb on + strand at 1557.643 kb on + strand at 1557.724 kb on + strand at 1557.968 kb on - strand, within AMB_RS07225 at 1557.968 kb on - strand, within AMB_RS07225 at 1557.968 kb on - strand, within AMB_RS07225 at 1557.968 kb on - strand, within AMB_RS07225
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,554,793 + AMB_RS07210 0.74 +0.2 1,554,793 + AMB_RS07210 0.74 +0.4 1,554,957 + AMB_RS07210 0.84 -0.2 1,554,957 + AMB_RS07210 0.84 +0.4 1,554,957 + AMB_RS07210 0.84 -1.2 1,554,957 + AMB_RS07210 0.84 -3.9 1,554,957 + AMB_RS07210 0.84 -0.2 1,554,958 - AMB_RS07210 0.84 +0.2 1,554,958 - AMB_RS07210 0.84 +0.8 1,554,958 - AMB_RS07210 0.84 -0.2 1,554,958 - AMB_RS07210 0.84 +1.4 1,555,162 + +0.4 1,555,162 + +0.4 1,555,241 - +0.5 1,555,416 + +0.5 1,555,538 + +0.7 1,555,538 + +0.3 1,555,538 + +0.0 1,555,538 + -1.4 1,555,539 - -0.3 1,555,539 - -0.4 1,555,539 - -0.0 1,555,605 + +0.4 1,555,605 + -3.5 1,555,606 - -0.2 1,555,833 + AMB_RS07215 0.21 +1.4 1,555,833 + AMB_RS07215 0.21 -0.1 1,555,834 - AMB_RS07215 0.21 +0.0 1,555,834 - AMB_RS07215 0.21 +0.0 1,555,834 - AMB_RS07215 0.21 -0.3 1,556,526 + AMB_RS07215 0.69 -1.6 1,556,527 - AMB_RS07215 0.69 +0.1 1,556,607 + AMB_RS07215 0.75 -0.6 1,556,656 - AMB_RS07215 0.78 -1.8 1,556,667 + AMB_RS07215 0.79 -3.0 1,556,668 - AMB_RS07215 0.79 -2.9 1,556,989 + +0.1 1,556,990 - +0.2 1,557,189 + AMB_RS07220 0.38 -0.6 1,557,189 + AMB_RS07220 0.38 -1.2 1,557,189 + AMB_RS07220 0.38 -0.6 1,557,189 + AMB_RS07220 0.38 -1.0 1,557,189 + AMB_RS07220 0.38 -1.0 1,557,190 - AMB_RS07220 0.38 -1.8 1,557,190 - AMB_RS07220 0.38 -0.2 1,557,190 - AMB_RS07220 0.38 -1.0 1,557,190 - AMB_RS07220 0.38 -1.2 1,557,190 - AMB_RS07220 0.38 -2.2 1,557,190 - AMB_RS07220 0.38 +0.1 1,557,195 + AMB_RS07220 0.39 +0.3 1,557,444 + +0.6 1,557,444 + -1.4 1,557,445 - -1.4 1,557,489 + -1.0 1,557,549 + -0.2 1,557,550 - +0.4 1,557,553 - -1.6 1,557,553 - +0.2 1,557,553 - -3.0 1,557,565 + +0.4 1,557,566 - -0.2 1,557,566 - -1.2 1,557,566 - -3.0 1,557,572 - +0.2 1,557,573 + +0.7 1,557,643 + +0.0 1,557,724 + -1.2 1,557,968 - AMB_RS07225 0.24 -3.1 1,557,968 - AMB_RS07225 0.24 -0.6 1,557,968 - AMB_RS07225 0.24 -1.2 1,557,968 - AMB_RS07225 0.24 +0.4
Or see this region's nucleotide sequence