Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07165 and AMB_RS07170 are separated by 192 nucleotides AMB_RS07170 and AMB_RS07175 are separated by 46 nucleotides AMB_RS07175 and AMB_RS07180 are separated by 239 nucleotides
AMB_RS07165: AMB_RS07165 - hemerythrin, at 1,541,691 to 1,542,089
_RS07165
AMB_RS07170: AMB_RS07170 - hypothetical protein, at 1,542,282 to 1,542,716
_RS07170
AMB_RS07175: AMB_RS07175 - ATP-dependent helicase, at 1,542,763 to 1,544,676
_RS07175
AMB_RS07180: AMB_RS07180 - membrane protein, at 1,544,916 to 1,547,816
_RS07180
Position (kb)
1542
1543
1544
1545 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1541.844 kb on + strand, within AMB_RS07165 at 1541.853 kb on + strand, within AMB_RS07165 at 1541.854 kb on - strand, within AMB_RS07165 at 1542.193 kb on + strand at 1542.459 kb on + strand, within AMB_RS07170 at 1542.459 kb on + strand, within AMB_RS07170 at 1542.459 kb on + strand, within AMB_RS07170 at 1542.460 kb on - strand, within AMB_RS07170 at 1542.460 kb on - strand, within AMB_RS07170 at 1542.647 kb on + strand, within AMB_RS07170 at 1542.647 kb on + strand, within AMB_RS07170 at 1542.714 kb on + strand at 1542.714 kb on + strand at 1542.714 kb on + strand at 1542.715 kb on - strand at 1542.715 kb on - strand at 1542.715 kb on - strand at 1542.751 kb on - strand at 1542.751 kb on - strand at 1543.171 kb on + strand, within AMB_RS07175 at 1543.216 kb on + strand, within AMB_RS07175 at 1543.217 kb on - strand, within AMB_RS07175 at 1543.447 kb on + strand, within AMB_RS07175 at 1543.448 kb on - strand, within AMB_RS07175 at 1543.448 kb on - strand, within AMB_RS07175 at 1543.448 kb on - strand, within AMB_RS07175 at 1543.572 kb on + strand, within AMB_RS07175 at 1543.573 kb on - strand, within AMB_RS07175 at 1543.631 kb on - strand, within AMB_RS07175 at 1543.631 kb on - strand, within AMB_RS07175 at 1543.777 kb on + strand, within AMB_RS07175 at 1544.404 kb on + strand, within AMB_RS07175 at 1544.404 kb on + strand, within AMB_RS07175 at 1544.404 kb on + strand, within AMB_RS07175 at 1544.404 kb on + strand, within AMB_RS07175 at 1544.404 kb on + strand, within AMB_RS07175 at 1544.405 kb on - strand, within AMB_RS07175 at 1544.405 kb on - strand, within AMB_RS07175 at 1544.485 kb on + strand at 1544.485 kb on + strand at 1544.485 kb on + strand at 1544.485 kb on + strand at 1544.485 kb on + strand at 1544.485 kb on + strand at 1544.486 kb on - strand at 1544.486 kb on - strand at 1544.486 kb on - strand at 1544.486 kb on - strand at 1544.486 kb on - strand at 1544.674 kb on + strand at 1545.324 kb on + strand, within AMB_RS07180 at 1545.671 kb on - strand, within AMB_RS07180
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,541,844 + AMB_RS07165 0.38 +0.1 1,541,853 + AMB_RS07165 0.41 -0.2 1,541,854 - AMB_RS07165 0.41 +0.3 1,542,193 + +0.2 1,542,459 + AMB_RS07170 0.41 -1.0 1,542,459 + AMB_RS07170 0.41 -2.0 1,542,459 + AMB_RS07170 0.41 -0.6 1,542,460 - AMB_RS07170 0.41 -0.1 1,542,460 - AMB_RS07170 0.41 +0.3 1,542,647 + AMB_RS07170 0.84 +0.2 1,542,647 + AMB_RS07170 0.84 +1.4 1,542,714 + -1.0 1,542,714 + -1.6 1,542,714 + -2.0 1,542,715 - -2.1 1,542,715 - -0.2 1,542,715 - +0.0 1,542,751 - -0.1 1,542,751 - +0.4 1,543,171 + AMB_RS07175 0.21 +0.3 1,543,216 + AMB_RS07175 0.24 +1.3 1,543,217 - AMB_RS07175 0.24 -1.2 1,543,447 + AMB_RS07175 0.36 -0.2 1,543,448 - AMB_RS07175 0.36 -0.6 1,543,448 - AMB_RS07175 0.36 -0.0 1,543,448 - AMB_RS07175 0.36 +0.4 1,543,572 + AMB_RS07175 0.42 +0.4 1,543,573 - AMB_RS07175 0.42 +0.4 1,543,631 - AMB_RS07175 0.45 -0.3 1,543,631 - AMB_RS07175 0.45 +1.2 1,543,777 + AMB_RS07175 0.53 -1.6 1,544,404 + AMB_RS07175 0.86 -0.1 1,544,404 + AMB_RS07175 0.86 -0.6 1,544,404 + AMB_RS07175 0.86 -2.1 1,544,404 + AMB_RS07175 0.86 +0.2 1,544,404 + AMB_RS07175 0.86 +0.2 1,544,405 - AMB_RS07175 0.86 +0.0 1,544,405 - AMB_RS07175 0.86 -1.4 1,544,485 + +0.1 1,544,485 + -0.1 1,544,485 + +0.2 1,544,485 + +0.2 1,544,485 + +0.0 1,544,485 + -2.8 1,544,486 - -0.2 1,544,486 - -3.0 1,544,486 - +1.4 1,544,486 - +0.1 1,544,486 - -0.2 1,544,674 + -1.6 1,545,324 + AMB_RS07180 0.14 -1.4 1,545,671 - AMB_RS07180 0.26 +0.6
Or see this region's nucleotide sequence