Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07105 and AMB_RS07110 are separated by 4 nucleotides AMB_RS07110 and AMB_RS07115 are separated by 106 nucleotides AMB_RS07115 and AMB_RS07120 are separated by 9 nucleotides
AMB_RS07105: AMB_RS07105 - Lrp/AsnC family transcriptional regulator, at 1,528,857 to 1,529,363
_RS07105
AMB_RS07110: AMB_RS07110 - Lrp/AsnC family transcriptional regulator, at 1,529,368 to 1,529,811
_RS07110
AMB_RS07115: AMB_RS07115 - Lrp/AsnC family transcriptional regulator, at 1,529,918 to 1,530,400
_RS07115
AMB_RS07120: AMB_RS07120 - heme d1 biosynthesis radical SAM protein NirJ, at 1,530,410 to 1,531,552
_RS07120
Position (kb)
1529
1530
1531 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1529.181 kb on + strand, within AMB_RS07105 at 1529.181 kb on + strand, within AMB_RS07105 at 1529.182 kb on - strand, within AMB_RS07105 at 1529.182 kb on - strand, within AMB_RS07105 at 1529.272 kb on - strand, within AMB_RS07105 at 1529.361 kb on + strand at 1529.650 kb on + strand, within AMB_RS07110 at 1529.650 kb on + strand, within AMB_RS07110 at 1529.650 kb on + strand, within AMB_RS07110 at 1529.785 kb on + strand at 1529.785 kb on + strand at 1529.785 kb on + strand at 1529.786 kb on - strand at 1529.913 kb on - strand at 1529.913 kb on - strand at 1529.999 kb on + strand, within AMB_RS07115 at 1530.111 kb on - strand, within AMB_RS07115 at 1530.111 kb on - strand, within AMB_RS07115 at 1530.122 kb on + strand, within AMB_RS07115 at 1530.123 kb on - strand, within AMB_RS07115 at 1530.219 kb on - strand, within AMB_RS07115 at 1530.252 kb on - strand, within AMB_RS07115 at 1530.398 kb on + strand at 1530.398 kb on + strand at 1530.399 kb on - strand at 1530.539 kb on + strand, within AMB_RS07120 at 1530.539 kb on + strand, within AMB_RS07120 at 1530.540 kb on - strand, within AMB_RS07120 at 1530.713 kb on + strand, within AMB_RS07120 at 1530.713 kb on + strand, within AMB_RS07120 at 1530.714 kb on - strand, within AMB_RS07120 at 1530.788 kb on + strand, within AMB_RS07120 at 1530.816 kb on - strand, within AMB_RS07120 at 1531.014 kb on - strand, within AMB_RS07120 at 1531.014 kb on - strand, within AMB_RS07120 at 1531.082 kb on + strand, within AMB_RS07120 at 1531.082 kb on + strand, within AMB_RS07120 at 1531.083 kb on - strand, within AMB_RS07120 at 1531.083 kb on - strand, within AMB_RS07120 at 1531.166 kb on + strand, within AMB_RS07120 at 1531.166 kb on + strand, within AMB_RS07120
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,529,181 + AMB_RS07105 0.64 -1.4 1,529,181 + AMB_RS07105 0.64 -0.4 1,529,182 - AMB_RS07105 0.64 -2.2 1,529,182 - AMB_RS07105 0.64 -0.6 1,529,272 - AMB_RS07105 0.82 -1.5 1,529,361 + +0.4 1,529,650 + AMB_RS07110 0.64 -0.1 1,529,650 + AMB_RS07110 0.64 -1.2 1,529,650 + AMB_RS07110 0.64 -2.0 1,529,785 + -1.8 1,529,785 + -2.1 1,529,785 + -2.9 1,529,786 - -0.6 1,529,913 - +0.3 1,529,913 - -0.8 1,529,999 + AMB_RS07115 0.17 -1.5 1,530,111 - AMB_RS07115 0.40 -1.5 1,530,111 - AMB_RS07115 0.40 -1.0 1,530,122 + AMB_RS07115 0.42 -2.0 1,530,123 - AMB_RS07115 0.42 -0.2 1,530,219 - AMB_RS07115 0.62 -0.4 1,530,252 - AMB_RS07115 0.69 -1.6 1,530,398 + -1.0 1,530,398 + +1.4 1,530,399 - -2.3 1,530,539 + AMB_RS07120 0.11 -1.3 1,530,539 + AMB_RS07120 0.11 -2.2 1,530,540 - AMB_RS07120 0.11 +0.4 1,530,713 + AMB_RS07120 0.27 -1.5 1,530,713 + AMB_RS07120 0.27 -1.2 1,530,714 - AMB_RS07120 0.27 -2.3 1,530,788 + AMB_RS07120 0.33 -2.1 1,530,816 - AMB_RS07120 0.36 +0.2 1,531,014 - AMB_RS07120 0.53 -1.8 1,531,014 - AMB_RS07120 0.53 -2.5 1,531,082 + AMB_RS07120 0.59 -0.6 1,531,082 + AMB_RS07120 0.59 -0.4 1,531,083 - AMB_RS07120 0.59 -0.6 1,531,083 - AMB_RS07120 0.59 -0.8 1,531,166 + AMB_RS07120 0.66 -0.2 1,531,166 + AMB_RS07120 0.66 -0.2
Or see this region's nucleotide sequence