Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07035 and AMB_RS07040 overlap by 191 nucleotides AMB_RS07040 and AMB_RS07045 are separated by 78 nucleotides AMB_RS07045 and AMB_RS07050 are separated by 64 nucleotides
AMB_RS07035: AMB_RS07035 - gamma-glutamyltransferase, at 1,509,850 to 1,511,481
_RS07035
AMB_RS07040: AMB_RS07040 - peptidase, at 1,511,291 to 1,512,838
_RS07040
AMB_RS07045: AMB_RS07045 - protein disulfide-isomerase, at 1,512,917 to 1,513,687
_RS07045
AMB_RS07050: AMB_RS07050 - hypothetical protein, at 1,513,752 to 1,515,953
_RS07050
Position (kb)
1511
1512
1513 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1510.436 kb on + strand, within AMB_RS07035 at 1510.436 kb on + strand, within AMB_RS07035 at 1510.832 kb on - strand, within AMB_RS07035 at 1510.855 kb on + strand, within AMB_RS07035 at 1510.855 kb on + strand, within AMB_RS07035 at 1510.855 kb on + strand, within AMB_RS07035 at 1510.855 kb on + strand, within AMB_RS07035 at 1510.855 kb on + strand, within AMB_RS07035 at 1510.856 kb on - strand, within AMB_RS07035 at 1510.856 kb on - strand, within AMB_RS07035 at 1511.042 kb on + strand, within AMB_RS07035 at 1511.060 kb on + strand, within AMB_RS07035 at 1511.160 kb on + strand, within AMB_RS07035 at 1511.161 kb on - strand, within AMB_RS07035 at 1511.167 kb on + strand, within AMB_RS07035 at 1511.167 kb on + strand, within AMB_RS07035 at 1511.168 kb on - strand, within AMB_RS07035 at 1511.168 kb on - strand, within AMB_RS07035 at 1511.168 kb on - strand, within AMB_RS07035 at 1511.168 kb on - strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.170 kb on + strand, within AMB_RS07035 at 1511.171 kb on - strand, within AMB_RS07035 at 1511.171 kb on - strand, within AMB_RS07035 at 1511.171 kb on - strand, within AMB_RS07035 at 1511.232 kb on - strand, within AMB_RS07035 at 1511.232 kb on - strand, within AMB_RS07035 at 1511.232 kb on - strand, within AMB_RS07035 at 1511.232 kb on - strand, within AMB_RS07035 at 1512.263 kb on + strand, within AMB_RS07040 at 1512.390 kb on - strand, within AMB_RS07040 at 1512.680 kb on + strand, within AMB_RS07040 at 1512.836 kb on + strand at 1512.836 kb on + strand at 1512.836 kb on + strand at 1512.836 kb on + strand at 1512.837 kb on - strand at 1512.877 kb on + strand at 1512.877 kb on + strand at 1512.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,510,436 + AMB_RS07035 0.36 -0.3 1,510,436 + AMB_RS07035 0.36 +0.4 1,510,832 - AMB_RS07035 0.60 -2.0 1,510,855 + AMB_RS07035 0.62 +0.2 1,510,855 + AMB_RS07035 0.62 -2.7 1,510,855 + AMB_RS07035 0.62 -1.8 1,510,855 + AMB_RS07035 0.62 -1.2 1,510,855 + AMB_RS07035 0.62 +0.4 1,510,856 - AMB_RS07035 0.62 -1.8 1,510,856 - AMB_RS07035 0.62 -0.2 1,511,042 + AMB_RS07035 0.73 -0.3 1,511,060 + AMB_RS07035 0.74 -0.2 1,511,160 + AMB_RS07035 0.80 -1.2 1,511,161 - AMB_RS07035 0.80 -1.8 1,511,167 + AMB_RS07035 0.81 -0.2 1,511,167 + AMB_RS07035 0.81 -1.0 1,511,168 - AMB_RS07035 0.81 -1.2 1,511,168 - AMB_RS07035 0.81 -0.6 1,511,168 - AMB_RS07035 0.81 +0.6 1,511,168 - AMB_RS07035 0.81 -2.2 1,511,170 + AMB_RS07035 0.81 +0.1 1,511,170 + AMB_RS07035 0.81 +0.5 1,511,170 + AMB_RS07035 0.81 -2.5 1,511,170 + AMB_RS07035 0.81 -0.5 1,511,170 + AMB_RS07035 0.81 -2.0 1,511,170 + AMB_RS07035 0.81 +0.3 1,511,170 + AMB_RS07035 0.81 +0.7 1,511,170 + AMB_RS07035 0.81 +0.8 1,511,170 + AMB_RS07035 0.81 -0.3 1,511,170 + AMB_RS07035 0.81 -1.5 1,511,171 - AMB_RS07035 0.81 -1.5 1,511,171 - AMB_RS07035 0.81 -1.0 1,511,171 - AMB_RS07035 0.81 -0.4 1,511,232 - AMB_RS07035 0.85 -1.5 1,511,232 - AMB_RS07035 0.85 -0.2 1,511,232 - AMB_RS07035 0.85 -0.6 1,511,232 - AMB_RS07035 0.85 +0.5 1,512,263 + AMB_RS07040 0.63 -1.0 1,512,390 - AMB_RS07040 0.71 -1.6 1,512,680 + AMB_RS07040 0.90 -4.3 1,512,836 + -2.2 1,512,836 + +0.4 1,512,836 + +0.2 1,512,836 + +0.3 1,512,837 - +0.2 1,512,877 + -2.3 1,512,877 + -2.2 1,512,906 + -1.0
Or see this region's nucleotide sequence