Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06395 and AMB_RS06400 are separated by 63 nucleotides AMB_RS06400 and AMB_RS06405 overlap by 4 nucleotides AMB_RS06405 and AMB_RS06410 overlap by 4 nucleotides AMB_RS06410 and AMB_RS06415 are separated by 65 nucleotides
AMB_RS06395: AMB_RS06395 - ATP-dependent helicase HrpB, at 1,342,073 to 1,344,568
_RS06395
AMB_RS06400: AMB_RS06400 - EF-hand domain-containing protein, at 1,344,632 to 1,345,318
_RS06400
AMB_RS06405: AMB_RS06405 - hypothetical protein, at 1,345,315 to 1,346,361
_RS06405
AMB_RS06410: AMB_RS06410 - metal-sulfur cluster assembly factor, at 1,346,358 to 1,346,663
_RS06410
AMB_RS06415: AMB_RS06415 - PAS domain S-box protein, at 1,346,729 to 1,348,735
_RS06415
Position (kb)
1345
1346
1347 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1344.464 kb on + strand at 1344.464 kb on + strand at 1344.464 kb on + strand at 1344.464 kb on + strand at 1344.465 kb on - strand at 1344.465 kb on - strand at 1344.465 kb on - strand at 1344.465 kb on - strand at 1344.465 kb on - strand at 1344.567 kb on - strand at 1344.618 kb on + strand at 1344.618 kb on + strand at 1344.618 kb on + strand at 1344.673 kb on + strand at 1344.673 kb on + strand at 1344.818 kb on + strand, within AMB_RS06400 at 1344.819 kb on - strand, within AMB_RS06400 at 1344.819 kb on - strand, within AMB_RS06400 at 1344.819 kb on - strand, within AMB_RS06400 at 1344.995 kb on + strand, within AMB_RS06400 at 1344.996 kb on - strand, within AMB_RS06400 at 1344.996 kb on - strand, within AMB_RS06400 at 1345.035 kb on - strand, within AMB_RS06400 at 1345.337 kb on - strand at 1345.478 kb on + strand, within AMB_RS06405 at 1345.478 kb on + strand, within AMB_RS06405 at 1345.478 kb on + strand, within AMB_RS06405 at 1345.479 kb on - strand, within AMB_RS06405 at 1345.479 kb on - strand, within AMB_RS06405 at 1345.493 kb on + strand, within AMB_RS06405 at 1345.494 kb on - strand, within AMB_RS06405 at 1345.494 kb on - strand, within AMB_RS06405 at 1345.596 kb on - strand, within AMB_RS06405 at 1345.688 kb on + strand, within AMB_RS06405 at 1346.153 kb on + strand, within AMB_RS06405 at 1346.501 kb on - strand, within AMB_RS06410 at 1346.545 kb on + strand, within AMB_RS06410 at 1346.545 kb on + strand, within AMB_RS06410 at 1346.545 kb on + strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.546 kb on - strand, within AMB_RS06410 at 1346.695 kb on + strand at 1346.696 kb on - strand at 1347.006 kb on + strand, within AMB_RS06415 at 1347.006 kb on + strand, within AMB_RS06415 at 1347.006 kb on + strand, within AMB_RS06415 at 1347.006 kb on + strand, within AMB_RS06415 at 1347.007 kb on - strand, within AMB_RS06415 at 1347.007 kb on - strand, within AMB_RS06415 at 1347.007 kb on - strand, within AMB_RS06415 at 1347.007 kb on - strand, within AMB_RS06415 at 1347.215 kb on + strand, within AMB_RS06415 at 1347.261 kb on + strand, within AMB_RS06415 at 1347.261 kb on + strand, within AMB_RS06415 at 1347.261 kb on + strand, within AMB_RS06415 at 1347.261 kb on + strand, within AMB_RS06415 at 1347.261 kb on + strand, within AMB_RS06415 at 1347.262 kb on - strand, within AMB_RS06415 at 1347.262 kb on - strand, within AMB_RS06415 at 1347.262 kb on - strand, within AMB_RS06415 at 1347.262 kb on - strand, within AMB_RS06415 at 1347.262 kb on - strand, within AMB_RS06415 at 1347.262 kb on - strand, within AMB_RS06415 at 1347.268 kb on + strand, within AMB_RS06415 at 1347.268 kb on + strand, within AMB_RS06415 at 1347.268 kb on + strand, within AMB_RS06415 at 1347.268 kb on + strand, within AMB_RS06415 at 1347.269 kb on - strand, within AMB_RS06415 at 1347.269 kb on - strand, within AMB_RS06415 at 1347.269 kb on - strand, within AMB_RS06415 at 1347.269 kb on - strand, within AMB_RS06415 at 1347.269 kb on - strand, within AMB_RS06415 at 1347.270 kb on + strand, within AMB_RS06415 at 1347.270 kb on + strand, within AMB_RS06415 at 1347.270 kb on + strand, within AMB_RS06415 at 1347.270 kb on + strand, within AMB_RS06415 at 1347.271 kb on - strand, within AMB_RS06415 at 1347.271 kb on - strand, within AMB_RS06415
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,344,464 + +1.1 1,344,464 + -1.2 1,344,464 + -0.2 1,344,464 + -1.2 1,344,465 - +0.5 1,344,465 - -0.6 1,344,465 - +0.3 1,344,465 - +1.1 1,344,465 - -0.7 1,344,567 - -0.9 1,344,618 + +0.1 1,344,618 + -0.6 1,344,618 + -1.2 1,344,673 + -2.6 1,344,673 + +0.4 1,344,818 + AMB_RS06400 0.27 -0.6 1,344,819 - AMB_RS06400 0.27 -0.2 1,344,819 - AMB_RS06400 0.27 -0.6 1,344,819 - AMB_RS06400 0.27 -0.5 1,344,995 + AMB_RS06400 0.53 +0.1 1,344,996 - AMB_RS06400 0.53 -0.0 1,344,996 - AMB_RS06400 0.53 +0.7 1,345,035 - AMB_RS06400 0.59 +0.3 1,345,337 - -0.9 1,345,478 + AMB_RS06405 0.16 -0.6 1,345,478 + AMB_RS06405 0.16 +0.7 1,345,478 + AMB_RS06405 0.16 -0.2 1,345,479 - AMB_RS06405 0.16 -2.2 1,345,479 - AMB_RS06405 0.16 -1.6 1,345,493 + AMB_RS06405 0.17 +0.4 1,345,494 - AMB_RS06405 0.17 -0.8 1,345,494 - AMB_RS06405 0.17 -1.8 1,345,596 - AMB_RS06405 0.27 -2.2 1,345,688 + AMB_RS06405 0.36 -1.2 1,346,153 + AMB_RS06405 0.80 -0.8 1,346,501 - AMB_RS06410 0.47 +0.4 1,346,545 + AMB_RS06410 0.61 +0.4 1,346,545 + AMB_RS06410 0.61 -0.6 1,346,545 + AMB_RS06410 0.61 -0.1 1,346,546 - AMB_RS06410 0.61 -0.0 1,346,546 - AMB_RS06410 0.61 -1.2 1,346,546 - AMB_RS06410 0.61 -3.0 1,346,546 - AMB_RS06410 0.61 +0.4 1,346,546 - AMB_RS06410 0.61 -0.6 1,346,546 - AMB_RS06410 0.61 -1.4 1,346,546 - AMB_RS06410 0.61 -2.2 1,346,695 + -1.4 1,346,696 - -0.2 1,347,006 + AMB_RS06415 0.14 -1.8 1,347,006 + AMB_RS06415 0.14 +0.4 1,347,006 + AMB_RS06415 0.14 -0.9 1,347,006 + AMB_RS06415 0.14 +0.4 1,347,007 - AMB_RS06415 0.14 -0.6 1,347,007 - AMB_RS06415 0.14 +0.4 1,347,007 - AMB_RS06415 0.14 +0.3 1,347,007 - AMB_RS06415 0.14 -1.2 1,347,215 + AMB_RS06415 0.24 -2.2 1,347,261 + AMB_RS06415 0.27 -0.9 1,347,261 + AMB_RS06415 0.27 +0.7 1,347,261 + AMB_RS06415 0.27 +0.2 1,347,261 + AMB_RS06415 0.27 +0.4 1,347,261 + AMB_RS06415 0.27 -1.8 1,347,262 - AMB_RS06415 0.27 +0.4 1,347,262 - AMB_RS06415 0.27 -0.3 1,347,262 - AMB_RS06415 0.27 +0.9 1,347,262 - AMB_RS06415 0.27 -1.4 1,347,262 - AMB_RS06415 0.27 +0.1 1,347,262 - AMB_RS06415 0.27 +0.5 1,347,268 + AMB_RS06415 0.27 +0.2 1,347,268 + AMB_RS06415 0.27 +0.4 1,347,268 + AMB_RS06415 0.27 +0.3 1,347,268 + AMB_RS06415 0.27 -0.7 1,347,269 - AMB_RS06415 0.27 -1.6 1,347,269 - AMB_RS06415 0.27 -0.2 1,347,269 - AMB_RS06415 0.27 -0.2 1,347,269 - AMB_RS06415 0.27 -1.2 1,347,269 - AMB_RS06415 0.27 -1.4 1,347,270 + AMB_RS06415 0.27 +0.4 1,347,270 + AMB_RS06415 0.27 +0.0 1,347,270 + AMB_RS06415 0.27 +0.1 1,347,270 + AMB_RS06415 0.27 +0.1 1,347,271 - AMB_RS06415 0.27 -1.8 1,347,271 - AMB_RS06415 0.27 -1.2
Or see this region's nucleotide sequence