Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05750 and AMB_RS05755 overlap by 4 nucleotides AMB_RS05755 and AMB_RS05760 are separated by 1 nucleotides AMB_RS05760 and AMB_RS05765 are separated by 214 nucleotides AMB_RS05765 and AMB_RS05770 are separated by 451 nucleotides
AMB_RS05750: AMB_RS05750 - DUF3732 domain-containing protein, at 1,209,550 to 1,211,505
_RS05750
AMB_RS05755: AMB_RS05755 - hypothetical protein, at 1,211,502 to 1,212,014
_RS05755
AMB_RS05760: AMB_RS05760 - hypothetical protein, at 1,212,016 to 1,213,215
_RS05760
AMB_RS05765: AMB_RS05765 - hypothetical protein, at 1,213,430 to 1,213,732
_RS05765
AMB_RS05770: AMB_RS05770 - AraC family transcriptional regulator, at 1,214,184 to 1,215,185
_RS05770
Position (kb)
1212
1213
1214 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1211.213 kb on + strand, within AMB_RS05750 at 1211.213 kb on + strand, within AMB_RS05750 at 1211.213 kb on + strand, within AMB_RS05750 at 1211.213 kb on + strand, within AMB_RS05750 at 1211.213 kb on + strand, within AMB_RS05750 at 1211.214 kb on - strand, within AMB_RS05750 at 1211.214 kb on - strand, within AMB_RS05750 at 1211.214 kb on - strand, within AMB_RS05750 at 1211.324 kb on - strand at 1211.421 kb on + strand at 1211.422 kb on - strand at 1211.474 kb on + strand at 1211.613 kb on + strand, within AMB_RS05755 at 1211.613 kb on + strand, within AMB_RS05755 at 1211.801 kb on + strand, within AMB_RS05755 at 1211.801 kb on + strand, within AMB_RS05755 at 1211.802 kb on - strand, within AMB_RS05755 at 1211.802 kb on - strand, within AMB_RS05755 at 1211.802 kb on - strand, within AMB_RS05755 at 1211.906 kb on - strand, within AMB_RS05755 at 1212.092 kb on + strand at 1212.092 kb on + strand at 1212.092 kb on + strand at 1212.092 kb on + strand at 1212.092 kb on + strand at 1212.092 kb on + strand at 1212.093 kb on - strand at 1212.093 kb on - strand at 1212.093 kb on - strand at 1212.093 kb on - strand at 1212.093 kb on - strand at 1212.164 kb on + strand, within AMB_RS05760 at 1212.164 kb on + strand, within AMB_RS05760 at 1212.165 kb on - strand, within AMB_RS05760 at 1212.322 kb on - strand, within AMB_RS05760 at 1212.425 kb on + strand, within AMB_RS05760 at 1212.426 kb on - strand, within AMB_RS05760 at 1212.639 kb on + strand, within AMB_RS05760 at 1212.639 kb on + strand, within AMB_RS05760 at 1212.640 kb on - strand, within AMB_RS05760 at 1212.764 kb on + strand, within AMB_RS05760 at 1212.765 kb on - strand, within AMB_RS05760 at 1212.843 kb on + strand, within AMB_RS05760 at 1212.843 kb on + strand, within AMB_RS05760 at 1212.995 kb on + strand, within AMB_RS05760 at 1212.995 kb on + strand, within AMB_RS05760 at 1212.996 kb on - strand, within AMB_RS05760 at 1213.164 kb on - strand at 1213.250 kb on - strand at 1213.250 kb on - strand at 1213.250 kb on - strand at 1213.393 kb on - strand at 1213.422 kb on + strand at 1213.422 kb on + strand at 1213.423 kb on - strand at 1213.423 kb on - strand at 1213.499 kb on - strand, within AMB_RS05765 at 1213.725 kb on + strand at 1213.725 kb on + strand at 1213.726 kb on - strand at 1213.726 kb on - strand at 1213.726 kb on - strand at 1213.726 kb on - strand at 1213.726 kb on - strand at 1213.840 kb on + strand at 1213.911 kb on - strand at 1213.947 kb on + strand at 1213.947 kb on + strand at 1213.947 kb on + strand at 1213.948 kb on - strand at 1213.948 kb on - strand at 1213.948 kb on - strand at 1213.948 kb on - strand at 1213.971 kb on - strand at 1213.979 kb on + strand at 1213.979 kb on + strand at 1213.979 kb on + strand at 1213.980 kb on - strand at 1213.980 kb on - strand at 1213.980 kb on - strand at 1213.980 kb on - strand at 1214.013 kb on + strand at 1214.014 kb on - strand at 1214.014 kb on - strand at 1214.096 kb on + strand at 1214.097 kb on - strand at 1214.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,211,213 + AMB_RS05750 0.85 -0.4 1,211,213 + AMB_RS05750 0.85 -0.0 1,211,213 + AMB_RS05750 0.85 -1.9 1,211,213 + AMB_RS05750 0.85 -0.2 1,211,213 + AMB_RS05750 0.85 -1.9 1,211,214 - AMB_RS05750 0.85 -0.2 1,211,214 - AMB_RS05750 0.85 -0.2 1,211,214 - AMB_RS05750 0.85 -3.2 1,211,324 - -0.2 1,211,421 + +0.2 1,211,422 - -1.2 1,211,474 + -0.2 1,211,613 + AMB_RS05755 0.22 -0.9 1,211,613 + AMB_RS05755 0.22 -2.4 1,211,801 + AMB_RS05755 0.58 -2.3 1,211,801 + AMB_RS05755 0.58 -0.2 1,211,802 - AMB_RS05755 0.58 -1.4 1,211,802 - AMB_RS05755 0.58 +0.1 1,211,802 - AMB_RS05755 0.58 +0.6 1,211,906 - AMB_RS05755 0.79 +0.4 1,212,092 + -0.2 1,212,092 + +0.1 1,212,092 + -1.4 1,212,092 + +0.3 1,212,092 + -0.2 1,212,092 + -1.2 1,212,093 - -1.9 1,212,093 - +0.3 1,212,093 - +0.1 1,212,093 - -0.9 1,212,093 - -0.1 1,212,164 + AMB_RS05760 0.12 -0.9 1,212,164 + AMB_RS05760 0.12 +1.4 1,212,165 - AMB_RS05760 0.12 +0.4 1,212,322 - AMB_RS05760 0.26 -1.6 1,212,425 + AMB_RS05760 0.34 -1.8 1,212,426 - AMB_RS05760 0.34 -2.7 1,212,639 + AMB_RS05760 0.52 -1.2 1,212,639 + AMB_RS05760 0.52 +1.4 1,212,640 - AMB_RS05760 0.52 -0.6 1,212,764 + AMB_RS05760 0.62 -1.6 1,212,765 - AMB_RS05760 0.62 -0.2 1,212,843 + AMB_RS05760 0.69 -1.9 1,212,843 + AMB_RS05760 0.69 +0.1 1,212,995 + AMB_RS05760 0.82 -2.5 1,212,995 + AMB_RS05760 0.82 +0.4 1,212,996 - AMB_RS05760 0.82 +0.2 1,213,164 - +0.2 1,213,250 - +0.4 1,213,250 - -0.9 1,213,250 - -0.5 1,213,393 - +0.4 1,213,422 + -0.9 1,213,422 + -3.3 1,213,423 - +0.2 1,213,423 - +0.4 1,213,499 - AMB_RS05765 0.23 +0.1 1,213,725 + -0.6 1,213,725 + -0.6 1,213,726 - -0.2 1,213,726 - -0.2 1,213,726 - -2.7 1,213,726 - -0.3 1,213,726 - +0.4 1,213,840 + -0.9 1,213,911 - -1.4 1,213,947 + +0.7 1,213,947 + -0.1 1,213,947 + -2.1 1,213,948 - +0.6 1,213,948 - -1.4 1,213,948 - -2.1 1,213,948 - +0.1 1,213,971 - -2.4 1,213,979 + -2.2 1,213,979 + -0.1 1,213,979 + -2.2 1,213,980 - -0.2 1,213,980 - -0.1 1,213,980 - +0.1 1,213,980 - -0.2 1,214,013 + -4.3 1,214,014 - +0.2 1,214,014 - +0.5 1,214,096 + -1.4 1,214,097 - -0.6 1,214,114 - -0.3
Or see this region's nucleotide sequence