Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05385 and AMB_RS05390 are separated by 50 nucleotides AMB_RS05390 and AMB_RS23990 are separated by 11 nucleotides AMB_RS23990 and AMB_RS23995 are separated by 6 nucleotides AMB_RS23995 and AMB_RS05400 are separated by 123 nucleotides
AMB_RS05385: AMB_RS05385 - cystathionine beta-synthase, at 1,116,070 to 1,117,098
_RS05385
AMB_RS05390: AMB_RS05390 - Rrf2 family transcriptional regulator, at 1,117,149 to 1,117,598
_RS05390
AMB_RS23990: AMB_RS23990 - aminotransferase class V-fold PLP-dependent enzyme, at 1,117,610 to 1,118,194
_RS23990
AMB_RS23995: AMB_RS23995 - hypothetical protein, at 1,118,201 to 1,118,419
_RS23995
AMB_RS05400: AMB_RS05400 - fumarate reductase/succinate dehydrogenase flavoprotein subunit, at 1,118,543 to 1,120,267
_RS05400
Position (kb)
1117
1118 Strain fitness (log2 ratio)
-2
-1
0
1 at 1117.587 kb on + strand at 1117.587 kb on + strand at 1117.601 kb on + strand at 1117.601 kb on + strand at 1117.726 kb on + strand, within AMB_RS23990 at 1117.726 kb on + strand, within AMB_RS23990 at 1117.726 kb on + strand, within AMB_RS23990 at 1117.726 kb on + strand, within AMB_RS23990 at 1117.726 kb on + strand, within AMB_RS23990 at 1117.726 kb on + strand, within AMB_RS23990 at 1117.727 kb on - strand, within AMB_RS23990 at 1117.727 kb on - strand, within AMB_RS23990 at 1117.727 kb on - strand, within AMB_RS23990 at 1117.727 kb on - strand, within AMB_RS23990 at 1118.132 kb on + strand, within AMB_RS23990 at 1118.132 kb on + strand, within AMB_RS23990 at 1118.132 kb on + strand, within AMB_RS23990 at 1118.133 kb on - strand, within AMB_RS23990 at 1118.133 kb on - strand, within AMB_RS23990 at 1118.133 kb on - strand, within AMB_RS23990 at 1118.192 kb on + strand at 1118.192 kb on + strand at 1118.193 kb on - strand at 1118.193 kb on - strand at 1118.193 kb on - strand at 1118.456 kb on + strand at 1118.456 kb on + strand at 1118.456 kb on + strand at 1118.456 kb on + strand at 1118.456 kb on + strand at 1118.456 kb on + strand at 1118.457 kb on - strand at 1118.457 kb on - strand at 1118.457 kb on - strand at 1118.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,117,587 + -0.5 1,117,587 + -1.6 1,117,601 + -0.9 1,117,601 + -1.2 1,117,726 + AMB_RS23990 0.20 +0.3 1,117,726 + AMB_RS23990 0.20 +0.2 1,117,726 + AMB_RS23990 0.20 -0.1 1,117,726 + AMB_RS23990 0.20 +0.5 1,117,726 + AMB_RS23990 0.20 +0.1 1,117,726 + AMB_RS23990 0.20 -0.2 1,117,727 - AMB_RS23990 0.20 -0.6 1,117,727 - AMB_RS23990 0.20 -2.3 1,117,727 - AMB_RS23990 0.20 -1.8 1,117,727 - AMB_RS23990 0.20 -1.8 1,118,132 + AMB_RS23990 0.89 -1.8 1,118,132 + AMB_RS23990 0.89 -0.0 1,118,132 + AMB_RS23990 0.89 -0.2 1,118,133 - AMB_RS23990 0.89 -0.0 1,118,133 - AMB_RS23990 0.89 +0.8 1,118,133 - AMB_RS23990 0.89 +0.4 1,118,192 + -0.0 1,118,192 + +0.2 1,118,193 - -1.9 1,118,193 - -0.6 1,118,193 - +0.5 1,118,456 + -0.3 1,118,456 + -0.2 1,118,456 + -0.6 1,118,456 + -0.6 1,118,456 + +0.1 1,118,456 + +0.4 1,118,457 - +0.2 1,118,457 - +0.4 1,118,457 - +0.5 1,118,457 - +0.5
Or see this region's nucleotide sequence