Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05100 and AMB_RS05105 are separated by 14 nucleotides AMB_RS05105 and AMB_RS05110 are separated by 167 nucleotides AMB_RS05110 and AMB_RS05115 are separated by 22 nucleotides AMB_RS05115 and AMB_RS23955 are separated by 407 nucleotides
AMB_RS05100: AMB_RS05100 - chemotaxis protein, at 1,055,119 to 1,056,429
_RS05100
AMB_RS05105: AMB_RS05105 - PAS domain S-box protein, at 1,056,444 to 1,056,983
_RS05105
AMB_RS05110: AMB_RS05110 - chemotaxis protein CheY, at 1,057,151 to 1,057,924
_RS05110
AMB_RS05115: AMB_RS05115 - hypothetical protein, at 1,057,947 to 1,058,159
_RS05115
AMB_RS23955: AMB_RS23955 - hypothetical protein, at 1,058,567 to 1,059,946
_RS23955
Position (kb)
1057
1058 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1056.394 kb on + strand at 1056.394 kb on + strand at 1056.394 kb on + strand at 1056.407 kb on + strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.482 kb on - strand at 1056.505 kb on - strand, within AMB_RS05105 at 1056.814 kb on - strand, within AMB_RS05105 at 1056.814 kb on - strand, within AMB_RS05105 at 1056.886 kb on + strand, within AMB_RS05105 at 1056.915 kb on - strand, within AMB_RS05105 at 1056.915 kb on - strand, within AMB_RS05105 at 1056.968 kb on + strand at 1056.969 kb on - strand at 1057.036 kb on - strand at 1057.126 kb on + strand at 1057.293 kb on + strand, within AMB_RS05110 at 1057.322 kb on + strand, within AMB_RS05110 at 1057.322 kb on + strand, within AMB_RS05110 at 1057.323 kb on - strand, within AMB_RS05110 at 1057.366 kb on + strand, within AMB_RS05110 at 1057.367 kb on - strand, within AMB_RS05110 at 1057.391 kb on + strand, within AMB_RS05110 at 1057.391 kb on + strand, within AMB_RS05110 at 1057.392 kb on - strand, within AMB_RS05110 at 1057.448 kb on + strand, within AMB_RS05110 at 1057.448 kb on + strand, within AMB_RS05110 at 1057.462 kb on - strand, within AMB_RS05110 at 1057.462 kb on - strand, within AMB_RS05110 at 1057.466 kb on - strand, within AMB_RS05110 at 1057.827 kb on + strand, within AMB_RS05110 at 1057.828 kb on - strand, within AMB_RS05110 at 1057.828 kb on - strand, within AMB_RS05110 at 1057.864 kb on + strand at 1058.005 kb on - strand, within AMB_RS05115 at 1058.059 kb on + strand, within AMB_RS05115 at 1058.060 kb on - strand, within AMB_RS05115 at 1058.098 kb on + strand, within AMB_RS05115 at 1058.098 kb on + strand, within AMB_RS05115 at 1058.099 kb on - strand, within AMB_RS05115 at 1058.099 kb on - strand, within AMB_RS05115 at 1058.113 kb on - strand, within AMB_RS05115 at 1058.197 kb on + strand at 1058.244 kb on + strand at 1058.244 kb on + strand at 1058.245 kb on - strand at 1058.245 kb on - strand at 1058.251 kb on + strand at 1058.252 kb on - strand at 1058.321 kb on - strand at 1058.390 kb on + strand at 1058.391 kb on - strand at 1058.391 kb on - strand at 1058.537 kb on + strand at 1058.578 kb on + strand at 1058.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,056,394 + +0.4 1,056,394 + +1.4 1,056,394 + +0.2 1,056,407 + -0.0 1,056,482 - -1.3 1,056,482 - -0.0 1,056,482 - -0.0 1,056,482 - +0.2 1,056,482 - -0.0 1,056,482 - -0.9 1,056,482 - +0.3 1,056,482 - +0.0 1,056,482 - -0.2 1,056,482 - +0.0 1,056,482 - +0.2 1,056,505 - AMB_RS05105 0.11 +1.3 1,056,814 - AMB_RS05105 0.69 -1.2 1,056,814 - AMB_RS05105 0.69 +1.4 1,056,886 + AMB_RS05105 0.82 -0.2 1,056,915 - AMB_RS05105 0.87 -1.2 1,056,915 - AMB_RS05105 0.87 -1.6 1,056,968 + -0.1 1,056,969 - -0.9 1,057,036 - +0.4 1,057,126 + -2.4 1,057,293 + AMB_RS05110 0.18 -0.1 1,057,322 + AMB_RS05110 0.22 +0.2 1,057,322 + AMB_RS05110 0.22 -0.3 1,057,323 - AMB_RS05110 0.22 -1.6 1,057,366 + AMB_RS05110 0.28 -0.9 1,057,367 - AMB_RS05110 0.28 +0.4 1,057,391 + AMB_RS05110 0.31 -1.2 1,057,391 + AMB_RS05110 0.31 -0.8 1,057,392 - AMB_RS05110 0.31 -0.7 1,057,448 + AMB_RS05110 0.38 -0.6 1,057,448 + AMB_RS05110 0.38 +0.2 1,057,462 - AMB_RS05110 0.40 -0.2 1,057,462 - AMB_RS05110 0.40 -0.6 1,057,466 - AMB_RS05110 0.41 -0.3 1,057,827 + AMB_RS05110 0.87 +0.1 1,057,828 - AMB_RS05110 0.87 -0.8 1,057,828 - AMB_RS05110 0.87 -1.4 1,057,864 + +1.0 1,058,005 - AMB_RS05115 0.27 -0.6 1,058,059 + AMB_RS05115 0.53 -3.1 1,058,060 - AMB_RS05115 0.53 -0.1 1,058,098 + AMB_RS05115 0.71 -1.4 1,058,098 + AMB_RS05115 0.71 -0.3 1,058,099 - AMB_RS05115 0.71 +1.4 1,058,099 - AMB_RS05115 0.71 -0.3 1,058,113 - AMB_RS05115 0.78 -0.4 1,058,197 + -0.9 1,058,244 + -0.5 1,058,244 + -1.7 1,058,245 - -2.7 1,058,245 - +0.4 1,058,251 + +0.3 1,058,252 - -0.0 1,058,321 - -1.6 1,058,390 + +0.4 1,058,391 - +0.1 1,058,391 - -2.2 1,058,537 + -1.6 1,058,578 + -2.7 1,058,672 - -2.4
Or see this region's nucleotide sequence