Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04850 and AMB_RS04855 are separated by 172 nucleotides AMB_RS04855 and AMB_RS04860 are separated by 377 nucleotides AMB_RS04860 and AMB_RS04865 are separated by 872 nucleotides
AMB_RS04850: AMB_RS04850 - hypothetical protein, at 1,010,231 to 1,010,710
_RS04850
AMB_RS04855: AMB_RS04855 - response regulator, at 1,010,883 to 1,011,287
_RS04855
AMB_RS04860: AMB_RS04860 - IS110 family transposase, at 1,011,665 to 1,012,693
_RS04860
AMB_RS04865: AMB_RS04865 - hypothetical protein, at 1,013,566 to 1,013,886
_RS04865
Position (kb)
1011
1012
1013 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1010.735 kb on - strand at 1010.807 kb on + strand at 1010.809 kb on + strand at 1010.812 kb on - strand at 1010.836 kb on + strand at 1011.008 kb on + strand, within AMB_RS04855 at 1011.008 kb on + strand, within AMB_RS04855 at 1011.009 kb on - strand, within AMB_RS04855 at 1011.009 kb on - strand, within AMB_RS04855 at 1011.041 kb on + strand, within AMB_RS04855 at 1011.041 kb on + strand, within AMB_RS04855 at 1011.041 kb on + strand, within AMB_RS04855 at 1011.086 kb on + strand, within AMB_RS04855 at 1011.137 kb on - strand, within AMB_RS04855 at 1011.175 kb on + strand, within AMB_RS04855 at 1011.175 kb on + strand, within AMB_RS04855 at 1011.175 kb on + strand, within AMB_RS04855 at 1011.175 kb on + strand, within AMB_RS04855 at 1011.176 kb on - strand, within AMB_RS04855 at 1011.382 kb on + strand at 1011.382 kb on + strand at 1011.382 kb on + strand at 1011.382 kb on + strand at 1011.489 kb on - strand at 1011.519 kb on + strand at 1011.579 kb on + strand at 1011.580 kb on - strand at 1011.911 kb on + strand, within AMB_RS04860 at 1011.911 kb on + strand, within AMB_RS04860 at 1011.911 kb on + strand, within AMB_RS04860 at 1011.912 kb on - strand, within AMB_RS04860 at 1011.912 kb on - strand, within AMB_RS04860 at 1011.912 kb on - strand, within AMB_RS04860 at 1012.106 kb on + strand, within AMB_RS04860 at 1012.107 kb on - strand, within AMB_RS04860 at 1012.107 kb on - strand, within AMB_RS04860 at 1012.107 kb on - strand, within AMB_RS04860 at 1012.488 kb on - strand, within AMB_RS04860 at 1012.528 kb on - strand, within AMB_RS04860 at 1012.664 kb on + strand at 1012.664 kb on + strand at 1012.665 kb on - strand at 1012.778 kb on + strand at 1012.778 kb on + strand at 1012.778 kb on + strand at 1012.778 kb on + strand at 1012.778 kb on + strand at 1012.779 kb on - strand at 1012.779 kb on - strand at 1012.801 kb on - strand at 1012.962 kb on + strand at 1012.963 kb on - strand at 1012.963 kb on - strand at 1012.963 kb on - strand at 1012.963 kb on - strand at 1012.963 kb on - strand at 1013.041 kb on + strand at 1013.041 kb on + strand at 1013.041 kb on + strand at 1013.041 kb on + strand at 1013.042 kb on - strand at 1013.042 kb on - strand at 1013.289 kb on + strand at 1013.473 kb on + strand at 1013.473 kb on + strand at 1013.474 kb on - strand at 1013.480 kb on + strand at 1013.480 kb on + strand at 1013.480 kb on + strand at 1013.481 kb on - strand at 1013.484 kb on + strand at 1013.484 kb on + strand at 1013.484 kb on + strand at 1013.485 kb on - strand at 1013.537 kb on + strand at 1013.538 kb on - strand at 1013.661 kb on - strand, within AMB_RS04865 at 1013.675 kb on + strand, within AMB_RS04865
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,010,735 - +0.3 1,010,807 + -0.4 1,010,809 + +0.3 1,010,812 - +0.5 1,010,836 + +0.2 1,011,008 + AMB_RS04855 0.31 -3.0 1,011,008 + AMB_RS04855 0.31 -0.4 1,011,009 - AMB_RS04855 0.31 +0.2 1,011,009 - AMB_RS04855 0.31 -0.1 1,011,041 + AMB_RS04855 0.39 +0.4 1,011,041 + AMB_RS04855 0.39 +0.4 1,011,041 + AMB_RS04855 0.39 -1.5 1,011,086 + AMB_RS04855 0.50 +1.0 1,011,137 - AMB_RS04855 0.63 -1.3 1,011,175 + AMB_RS04855 0.72 +0.2 1,011,175 + AMB_RS04855 0.72 -0.3 1,011,175 + AMB_RS04855 0.72 -1.7 1,011,175 + AMB_RS04855 0.72 -0.2 1,011,176 - AMB_RS04855 0.72 -2.2 1,011,382 + +0.2 1,011,382 + -0.2 1,011,382 + -3.2 1,011,382 + +0.3 1,011,489 - +0.0 1,011,519 + -3.2 1,011,579 + +0.2 1,011,580 - +0.5 1,011,911 + AMB_RS04860 0.24 -0.9 1,011,911 + AMB_RS04860 0.24 -1.1 1,011,911 + AMB_RS04860 0.24 +0.2 1,011,912 - AMB_RS04860 0.24 +0.8 1,011,912 - AMB_RS04860 0.24 -0.2 1,011,912 - AMB_RS04860 0.24 -0.9 1,012,106 + AMB_RS04860 0.43 -1.9 1,012,107 - AMB_RS04860 0.43 -1.3 1,012,107 - AMB_RS04860 0.43 -2.6 1,012,107 - AMB_RS04860 0.43 -0.1 1,012,488 - AMB_RS04860 0.80 +0.3 1,012,528 - AMB_RS04860 0.84 -0.9 1,012,664 + -0.1 1,012,664 + +0.5 1,012,665 - +0.0 1,012,778 + +0.1 1,012,778 + -1.3 1,012,778 + +0.0 1,012,778 + -1.1 1,012,778 + -0.9 1,012,779 - -0.3 1,012,779 - +0.2 1,012,801 - -0.1 1,012,962 + -1.9 1,012,963 - -2.1 1,012,963 - -0.1 1,012,963 - -1.1 1,012,963 - +0.6 1,012,963 - +1.5 1,013,041 + -0.1 1,013,041 + -2.9 1,013,041 + -0.1 1,013,041 + -1.3 1,013,042 - -0.5 1,013,042 - -0.5 1,013,289 + -1.1 1,013,473 + -0.1 1,013,473 + -0.7 1,013,474 - -1.5 1,013,480 + -0.9 1,013,480 + -2.1 1,013,480 + -0.3 1,013,481 - +0.2 1,013,484 + -0.1 1,013,484 + -2.0 1,013,484 + +0.2 1,013,485 - +0.0 1,013,537 + -0.0 1,013,538 - -1.9 1,013,661 - AMB_RS04865 0.30 -0.3 1,013,675 + AMB_RS04865 0.34 +0.6
Or see this region's nucleotide sequence