Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04620 and AMB_RS04625 are separated by 88 nucleotides AMB_RS04625 and AMB_RS04630 are separated by 105 nucleotides AMB_RS04630 and AMB_RS04635 are separated by 270 nucleotides
AMB_RS04620: AMB_RS04620 - 23S ribosomal RNA, at 966,932 to 969,685
_RS04620
AMB_RS04625: AMB_RS04625 - 5S ribosomal RNA, at 969,774 to 969,888
_RS04625
AMB_RS04630: AMB_RS04630 - tRNA-Met, at 969,994 to 970,070
_RS04630
AMB_RS04635: AMB_RS04635 - site-specific integrase, at 970,341 to 971,309
_RS04635
Position (kb) 969
970
971 Strain fitness (log2 ratio) -2
-1
0
1 at 970.030 kb on + strand, within AMB_RS04630 at 970.136 kb on - strand at 970.474 kb on - strand, within AMB_RS04635 at 970.518 kb on + strand, within AMB_RS04635 at 970.518 kb on + strand, within AMB_RS04635 at 970.519 kb on - strand, within AMB_RS04635 at 970.519 kb on - strand, within AMB_RS04635 at 970.519 kb on - strand, within AMB_RS04635 at 970.646 kb on + strand, within AMB_RS04635 at 970.668 kb on + strand, within AMB_RS04635 at 970.668 kb on + strand, within AMB_RS04635 at 970.670 kb on + strand, within AMB_RS04635 at 970.727 kb on + strand, within AMB_RS04635 at 970.727 kb on + strand, within AMB_RS04635 at 970.728 kb on - strand, within AMB_RS04635 at 971.014 kb on + strand, within AMB_RS04635
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 970,030 + AMB_RS04630 0.47 -2.0 970,136 - -0.1 970,474 - AMB_RS04635 0.14 -1.3 970,518 + AMB_RS04635 0.18 -0.2 970,518 + AMB_RS04635 0.18 -2.1 970,519 - AMB_RS04635 0.18 -1.7 970,519 - AMB_RS04635 0.18 -0.1 970,519 - AMB_RS04635 0.18 -1.1 970,646 + AMB_RS04635 0.31 +0.4 970,668 + AMB_RS04635 0.34 -2.6 970,668 + AMB_RS04635 0.34 +0.3 970,670 + AMB_RS04635 0.34 -1.1 970,727 + AMB_RS04635 0.40 -1.1 970,727 + AMB_RS04635 0.40 -1.5 970,728 - AMB_RS04635 0.40 +0.4 971,014 + AMB_RS04635 0.69 +0.7
Or see this region's nucleotide sequence