Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04475 and AMB_RS04480 are separated by 2 nucleotides AMB_RS04480 and AMB_RS04485 are separated by 60 nucleotides
AMB_RS04475: AMB_RS04475 - S41 family peptidase, at 938,874 to 940,430
_RS04475
AMB_RS04480: AMB_RS04480 - acyl-CoA dehydrogenase, at 940,433 to 941,263
_RS04480
AMB_RS04485: AMB_RS04485 - NAD(P)-dependent oxidoreductase, at 941,324 to 942,076
_RS04485
Position (kb) 940
941
942 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 939.672 kb on + strand, within AMB_RS04475 at 939.673 kb on - strand, within AMB_RS04475 at 939.997 kb on - strand, within AMB_RS04475 at 940.266 kb on - strand, within AMB_RS04475 at 940.344 kb on + strand at 940.577 kb on + strand, within AMB_RS04480 at 940.577 kb on + strand, within AMB_RS04480 at 940.578 kb on - strand, within AMB_RS04480 at 941.013 kb on - strand, within AMB_RS04480 at 941.125 kb on + strand, within AMB_RS04480 at 941.125 kb on + strand, within AMB_RS04480 at 941.126 kb on - strand, within AMB_RS04480 at 941.261 kb on + strand at 941.261 kb on + strand at 941.262 kb on - strand at 941.262 kb on - strand at 941.262 kb on - strand at 941.264 kb on + strand at 941.264 kb on + strand at 941.265 kb on - strand at 941.371 kb on + strand at 941.371 kb on + strand at 941.371 kb on + strand at 941.372 kb on - strand at 941.372 kb on - strand at 941.372 kb on - strand at 941.625 kb on + strand, within AMB_RS04485 at 941.625 kb on + strand, within AMB_RS04485 at 941.625 kb on + strand, within AMB_RS04485 at 941.626 kb on - strand, within AMB_RS04485 at 941.626 kb on - strand, within AMB_RS04485 at 942.083 kb on + strand at 942.084 kb on - strand at 942.085 kb on + strand at 942.085 kb on + strand at 942.085 kb on + strand at 942.086 kb on - strand at 942.203 kb on + strand at 942.217 kb on + strand at 942.249 kb on + strand at 942.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 939,672 + AMB_RS04475 0.51 -0.1 939,673 - AMB_RS04475 0.51 -2.4 939,997 - AMB_RS04475 0.72 -0.1 940,266 - AMB_RS04475 0.89 -1.3 940,344 + -0.9 940,577 + AMB_RS04480 0.17 +0.0 940,577 + AMB_RS04480 0.17 -0.5 940,578 - AMB_RS04480 0.17 +1.5 941,013 - AMB_RS04480 0.70 +0.1 941,125 + AMB_RS04480 0.83 +0.0 941,125 + AMB_RS04480 0.83 -1.1 941,126 - AMB_RS04480 0.83 -1.9 941,261 + -2.3 941,261 + -1.5 941,262 - +0.0 941,262 - +0.5 941,262 - -0.5 941,264 + +1.5 941,264 + +0.9 941,265 - -0.5 941,371 + -1.1 941,371 + -2.7 941,371 + -0.3 941,372 - +0.5 941,372 - -0.6 941,372 - -1.1 941,625 + AMB_RS04485 0.40 +0.0 941,625 + AMB_RS04485 0.40 -0.1 941,625 + AMB_RS04485 0.40 -0.9 941,626 - AMB_RS04485 0.40 -0.1 941,626 - AMB_RS04485 0.40 -2.5 942,083 + -2.0 942,084 - +0.6 942,085 + -2.7 942,085 + -1.3 942,085 + +0.1 942,086 - -0.3 942,203 + -0.1 942,217 + -3.1 942,249 + -0.9 942,249 + -0.9
Or see this region's nucleotide sequence