Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04370 and AMB_RS04375 overlap by 4 nucleotides AMB_RS04375 and AMB_RS04380 overlap by 4 nucleotides AMB_RS04380 and AMB_RS04385 are separated by 153 nucleotides
AMB_RS04370: AMB_RS04370 - sulfurtransferase TusA family protein, at 918,006 to 918,233
_RS04370
AMB_RS04375: AMB_RS04375 - N-acetylmuramoyl-L-alanine amidase, at 918,230 to 918,910
_RS04375
AMB_RS04380: AMB_RS04380 - EamA/RhaT family transporter, at 918,907 to 919,812
_RS04380
AMB_RS04385: AMB_RS04385 - elongation factor 3, at 919,966 to 921,780
_RS04385
Position (kb) 918
919
920 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 917.948 kb on + strand at 917.957 kb on - strand at 917.990 kb on + strand at 917.990 kb on + strand at 917.990 kb on + strand at 917.990 kb on + strand at 917.990 kb on + strand at 917.990 kb on + strand at 917.990 kb on + strand at 917.990 kb on + strand at 918.232 kb on - strand at 918.832 kb on - strand, within AMB_RS04375 at 919.122 kb on - strand, within AMB_RS04380 at 919.131 kb on + strand, within AMB_RS04380 at 919.131 kb on + strand, within AMB_RS04380 at 919.132 kb on - strand, within AMB_RS04380 at 919.132 kb on - strand, within AMB_RS04380 at 919.132 kb on - strand, within AMB_RS04380 at 919.132 kb on - strand, within AMB_RS04380 at 919.132 kb on - strand, within AMB_RS04380 at 919.132 kb on - strand, within AMB_RS04380 at 919.134 kb on - strand, within AMB_RS04380 at 919.134 kb on - strand, within AMB_RS04380 at 919.163 kb on + strand, within AMB_RS04380 at 919.883 kb on - strand at 920.014 kb on + strand at 920.014 kb on + strand at 920.014 kb on + strand at 920.015 kb on - strand at 920.015 kb on - strand at 920.015 kb on - strand at 920.015 kb on - strand at 920.093 kb on + strand at 920.093 kb on + strand at 920.093 kb on + strand at 920.093 kb on + strand at 920.094 kb on - strand at 920.094 kb on - strand at 920.149 kb on - strand, within AMB_RS04385 at 920.192 kb on + strand, within AMB_RS04385 at 920.192 kb on + strand, within AMB_RS04385 at 920.193 kb on - strand, within AMB_RS04385 at 920.223 kb on + strand, within AMB_RS04385 at 920.223 kb on + strand, within AMB_RS04385 at 920.223 kb on + strand, within AMB_RS04385 at 920.224 kb on - strand, within AMB_RS04385 at 920.294 kb on + strand, within AMB_RS04385 at 920.295 kb on - strand, within AMB_RS04385 at 920.315 kb on + strand, within AMB_RS04385 at 920.315 kb on + strand, within AMB_RS04385 at 920.315 kb on + strand, within AMB_RS04385 at 920.315 kb on + strand, within AMB_RS04385 at 920.315 kb on + strand, within AMB_RS04385 at 920.316 kb on - strand, within AMB_RS04385 at 920.316 kb on - strand, within AMB_RS04385 at 920.316 kb on - strand, within AMB_RS04385 at 920.603 kb on + strand, within AMB_RS04385 at 920.603 kb on + strand, within AMB_RS04385 at 920.604 kb on - strand, within AMB_RS04385 at 920.604 kb on - strand, within AMB_RS04385 at 920.717 kb on + strand, within AMB_RS04385 at 920.741 kb on - strand, within AMB_RS04385
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 917,948 + -2.4 917,957 - -0.2 917,990 + +0.9 917,990 + -0.3 917,990 + -1.5 917,990 + +0.1 917,990 + -2.7 917,990 + +0.5 917,990 + -1.9 917,990 + +0.5 918,232 - -0.2 918,832 - AMB_RS04375 0.88 +0.1 919,122 - AMB_RS04380 0.24 -2.3 919,131 + AMB_RS04380 0.25 -0.5 919,131 + AMB_RS04380 0.25 -0.2 919,132 - AMB_RS04380 0.25 -0.4 919,132 - AMB_RS04380 0.25 -1.3 919,132 - AMB_RS04380 0.25 -0.5 919,132 - AMB_RS04380 0.25 +0.3 919,132 - AMB_RS04380 0.25 +0.6 919,132 - AMB_RS04380 0.25 -1.3 919,134 - AMB_RS04380 0.25 +1.5 919,134 - AMB_RS04380 0.25 +0.4 919,163 + AMB_RS04380 0.28 +0.4 919,883 - +0.5 920,014 + -0.2 920,014 + -0.9 920,014 + +0.5 920,015 - +0.7 920,015 - +0.4 920,015 - +0.2 920,015 - -0.0 920,093 + -1.2 920,093 + -0.9 920,093 + -0.1 920,093 + +0.2 920,094 - +1.5 920,094 - -1.3 920,149 - AMB_RS04385 0.10 +0.3 920,192 + AMB_RS04385 0.12 -0.2 920,192 + AMB_RS04385 0.12 -2.3 920,193 - AMB_RS04385 0.13 -1.6 920,223 + AMB_RS04385 0.14 -0.1 920,223 + AMB_RS04385 0.14 -0.6 920,223 + AMB_RS04385 0.14 -0.1 920,224 - AMB_RS04385 0.14 -0.0 920,294 + AMB_RS04385 0.18 +0.5 920,295 - AMB_RS04385 0.18 -0.4 920,315 + AMB_RS04385 0.19 -1.4 920,315 + AMB_RS04385 0.19 +0.0 920,315 + AMB_RS04385 0.19 -0.0 920,315 + AMB_RS04385 0.19 -0.5 920,315 + AMB_RS04385 0.19 -2.9 920,316 - AMB_RS04385 0.19 -1.1 920,316 - AMB_RS04385 0.19 +0.1 920,316 - AMB_RS04385 0.19 -0.1 920,603 + AMB_RS04385 0.35 -0.7 920,603 + AMB_RS04385 0.35 -0.3 920,604 - AMB_RS04385 0.35 -0.9 920,604 - AMB_RS04385 0.35 -0.1 920,717 + AMB_RS04385 0.41 -0.1 920,741 - AMB_RS04385 0.43 +0.6
Or see this region's nucleotide sequence