Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04140 and AMB_RS04145 are separated by 109 nucleotides AMB_RS04145 and AMB_RS04150 are separated by 7 nucleotides AMB_RS04150 and AMB_RS04155 are separated by 126 nucleotides AMB_RS04155 and AMB_RS04160 overlap by 4 nucleotides
AMB_RS04140: AMB_RS04140 - TRAP-type C4-dicarboxylate transport system, at 867,413 to 868,528
_RS04140
AMB_RS04145: AMB_RS04145 - homoprotocatechuate degradation operon regulator HpaR, at 868,638 to 869,129
_RS04145
AMB_RS04150: AMB_RS04150 - acetylornithine transaminase, at 869,137 to 870,303
_RS04150
AMB_RS04155: AMB_RS04155 - heme-binding protein, at 870,430 to 870,822
_RS04155
AMB_RS04160: AMB_RS04160 - NAD-dependent succinate-semialdehyde dehydrogenase, at 870,819 to 872,318
_RS04160
Position (kb) 869
870
871 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 868.173 kb on + strand, within AMB_RS04140 at 868.459 kb on + strand at 868.459 kb on + strand at 868.460 kb on - strand at 868.460 kb on - strand at 868.531 kb on + strand at 868.531 kb on + strand at 868.532 kb on - strand at 868.532 kb on - strand at 869.010 kb on + strand, within AMB_RS04145 at 869.056 kb on + strand, within AMB_RS04145 at 869.057 kb on - strand, within AMB_RS04145 at 869.057 kb on - strand, within AMB_RS04145 at 869.057 kb on - strand, within AMB_RS04145 at 869.058 kb on + strand, within AMB_RS04145 at 869.058 kb on + strand, within AMB_RS04145 at 869.058 kb on + strand, within AMB_RS04145 at 869.059 kb on - strand, within AMB_RS04145 at 869.059 kb on - strand, within AMB_RS04145 at 869.418 kb on + strand, within AMB_RS04150 at 869.418 kb on + strand, within AMB_RS04150 at 869.418 kb on + strand, within AMB_RS04150 at 869.418 kb on + strand, within AMB_RS04150 at 869.419 kb on - strand, within AMB_RS04150 at 869.419 kb on - strand, within AMB_RS04150 at 869.419 kb on - strand, within AMB_RS04150 at 869.421 kb on + strand, within AMB_RS04150 at 869.568 kb on + strand, within AMB_RS04150 at 869.568 kb on + strand, within AMB_RS04150 at 869.568 kb on + strand, within AMB_RS04150 at 869.569 kb on - strand, within AMB_RS04150 at 869.569 kb on - strand, within AMB_RS04150 at 869.569 kb on - strand, within AMB_RS04150 at 869.572 kb on + strand, within AMB_RS04150 at 869.573 kb on - strand, within AMB_RS04150 at 869.573 kb on - strand, within AMB_RS04150 at 869.573 kb on - strand, within AMB_RS04150 at 869.573 kb on - strand, within AMB_RS04150 at 869.573 kb on - strand, within AMB_RS04150 at 869.621 kb on - strand, within AMB_RS04150 at 869.661 kb on + strand, within AMB_RS04150 at 869.661 kb on + strand, within AMB_RS04150 at 869.661 kb on + strand, within AMB_RS04150 at 869.661 kb on + strand, within AMB_RS04150 at 869.726 kb on - strand, within AMB_RS04150 at 870.156 kb on + strand, within AMB_RS04150 at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.844 kb on + strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 870.845 kb on - strand at 871.024 kb on + strand, within AMB_RS04160
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 868,173 + AMB_RS04140 0.68 +1.4 868,459 + -2.3 868,459 + -1.4 868,460 - +0.2 868,460 - +0.9 868,531 + +0.2 868,531 + -0.1 868,532 - -2.1 868,532 - +0.9 869,010 + AMB_RS04145 0.76 +1.4 869,056 + AMB_RS04145 0.85 -0.1 869,057 - AMB_RS04145 0.85 -1.1 869,057 - AMB_RS04145 0.85 -1.1 869,057 - AMB_RS04145 0.85 -0.0 869,058 + AMB_RS04145 0.85 -0.3 869,058 + AMB_RS04145 0.85 -0.6 869,058 + AMB_RS04145 0.85 -3.2 869,059 - AMB_RS04145 0.86 +1.4 869,059 - AMB_RS04145 0.86 -0.0 869,418 + AMB_RS04150 0.24 -2.0 869,418 + AMB_RS04150 0.24 +0.5 869,418 + AMB_RS04150 0.24 -0.9 869,418 + AMB_RS04150 0.24 +0.1 869,419 - AMB_RS04150 0.24 -0.1 869,419 - AMB_RS04150 0.24 -0.1 869,419 - AMB_RS04150 0.24 +0.3 869,421 + AMB_RS04150 0.24 +0.9 869,568 + AMB_RS04150 0.37 +1.1 869,568 + AMB_RS04150 0.37 -0.1 869,568 + AMB_RS04150 0.37 +0.5 869,569 - AMB_RS04150 0.37 -0.6 869,569 - AMB_RS04150 0.37 -0.6 869,569 - AMB_RS04150 0.37 +0.3 869,572 + AMB_RS04150 0.37 -1.4 869,573 - AMB_RS04150 0.37 +0.1 869,573 - AMB_RS04150 0.37 -0.6 869,573 - AMB_RS04150 0.37 -0.9 869,573 - AMB_RS04150 0.37 -0.6 869,573 - AMB_RS04150 0.37 +0.3 869,621 - AMB_RS04150 0.41 -1.4 869,661 + AMB_RS04150 0.45 -1.7 869,661 + AMB_RS04150 0.45 -0.9 869,661 + AMB_RS04150 0.45 -0.6 869,661 + AMB_RS04150 0.45 -0.8 869,726 - AMB_RS04150 0.50 -0.2 870,156 + AMB_RS04150 0.87 +0.0 870,844 + -1.1 870,844 + +0.0 870,844 + +0.4 870,844 + -0.6 870,844 + -3.7 870,844 + -0.6 870,844 + +0.4 870,844 + -1.9 870,845 - -1.4 870,845 - +0.4 870,845 - +1.2 870,845 - -0.4 870,845 - +0.1 870,845 - -1.6 870,845 - +0.2 871,024 + AMB_RS04160 0.14 -0.2
Or see this region's nucleotide sequence