Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03740 and AMB_RS03745 overlap by 1 nucleotides AMB_RS03745 and AMB_RS03750 are separated by 84 nucleotides AMB_RS03750 and AMB_RS03755 are separated by 7 nucleotides AMB_RS03755 and AMB_RS03760 are separated by 136 nucleotides
AMB_RS03740: AMB_RS03740 - signal transduction protein, at 791,538 to 793,577
_RS03740
AMB_RS03745: AMB_RS03745 - prolyl-tRNA synthetase associated domain-containing protein, at 793,577 to 794,068
_RS03745
AMB_RS03750: AMB_RS03750 - hypothetical protein, at 794,153 to 794,800
_RS03750
AMB_RS03755: AMB_RS03755 - hypothetical protein, at 794,808 to 795,296
_RS03755
AMB_RS03760: AMB_RS03760 - glutamine-hydrolyzing GMP synthase, at 795,433 to 796,989
_RS03760
Position (kb)
794
795 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 793.335 kb on + strand, within AMB_RS03740 at 793.336 kb on - strand, within AMB_RS03740 at 793.344 kb on + strand, within AMB_RS03740 at 793.344 kb on + strand, within AMB_RS03740 at 793.344 kb on + strand, within AMB_RS03740 at 793.344 kb on + strand, within AMB_RS03740 at 793.345 kb on - strand, within AMB_RS03740 at 793.345 kb on - strand, within AMB_RS03740 at 793.360 kb on - strand, within AMB_RS03740 at 793.360 kb on - strand, within AMB_RS03740 at 793.758 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.967 kb on + strand, within AMB_RS03745 at 793.968 kb on - strand, within AMB_RS03745 at 793.968 kb on - strand, within AMB_RS03745 at 793.968 kb on - strand, within AMB_RS03745 at 793.987 kb on + strand, within AMB_RS03745 at 793.987 kb on + strand, within AMB_RS03745 at 793.987 kb on + strand, within AMB_RS03745 at 793.987 kb on + strand, within AMB_RS03745 at 793.988 kb on - strand, within AMB_RS03745 at 793.988 kb on - strand, within AMB_RS03745 at 793.988 kb on - strand, within AMB_RS03745 at 793.988 kb on - strand, within AMB_RS03745 at 793.988 kb on - strand, within AMB_RS03745 at 794.102 kb on + strand at 794.103 kb on - strand at 794.103 kb on - strand at 794.151 kb on + strand at 794.151 kb on + strand at 794.151 kb on + strand at 794.152 kb on - strand at 794.163 kb on + strand at 794.163 kb on + strand at 794.163 kb on + strand at 794.163 kb on + strand at 794.163 kb on + strand at 794.163 kb on + strand at 794.164 kb on - strand at 794.164 kb on - strand at 794.164 kb on - strand at 794.354 kb on + strand, within AMB_RS03750 at 794.355 kb on - strand, within AMB_RS03750 at 794.396 kb on + strand, within AMB_RS03750 at 794.453 kb on + strand, within AMB_RS03750 at 794.453 kb on + strand, within AMB_RS03750 at 794.453 kb on + strand, within AMB_RS03750 at 794.454 kb on - strand, within AMB_RS03750 at 794.468 kb on + strand, within AMB_RS03750 at 794.468 kb on + strand, within AMB_RS03750 at 794.468 kb on + strand, within AMB_RS03750 at 794.468 kb on + strand, within AMB_RS03750 at 794.468 kb on + strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.469 kb on - strand, within AMB_RS03750 at 794.821 kb on + strand at 794.983 kb on + strand, within AMB_RS03755 at 794.983 kb on + strand, within AMB_RS03755 at 794.984 kb on - strand, within AMB_RS03755 at 795.043 kb on + strand, within AMB_RS03755 at 795.043 kb on + strand, within AMB_RS03755 at 795.043 kb on + strand, within AMB_RS03755 at 795.043 kb on + strand, within AMB_RS03755 at 795.043 kb on + strand, within AMB_RS03755 at 795.044 kb on - strand, within AMB_RS03755 at 795.044 kb on - strand, within AMB_RS03755 at 795.121 kb on + strand, within AMB_RS03755 at 795.121 kb on + strand, within AMB_RS03755 at 795.121 kb on + strand, within AMB_RS03755 at 795.121 kb on + strand, within AMB_RS03755 at 795.122 kb on - strand, within AMB_RS03755 at 795.122 kb on - strand, within AMB_RS03755 at 795.122 kb on - strand, within AMB_RS03755 at 795.122 kb on - strand, within AMB_RS03755 at 795.248 kb on - strand at 795.267 kb on + strand at 795.267 kb on + strand at 795.267 kb on + strand at 795.268 kb on - strand at 795.268 kb on - strand at 795.268 kb on - strand at 795.332 kb on - strand at 795.332 kb on - strand at 795.371 kb on + strand at 795.371 kb on + strand at 795.371 kb on + strand at 795.372 kb on - strand at 795.372 kb on - strand at 795.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 793,335 + AMB_RS03740 0.88 +0.5 793,336 - AMB_RS03740 0.88 +0.1 793,344 + AMB_RS03740 0.89 -0.5 793,344 + AMB_RS03740 0.89 -1.3 793,344 + AMB_RS03740 0.89 +0.0 793,344 + AMB_RS03740 0.89 -1.2 793,345 - AMB_RS03740 0.89 -0.1 793,345 - AMB_RS03740 0.89 -2.0 793,360 - AMB_RS03740 0.89 +1.5 793,360 - AMB_RS03740 0.89 -0.3 793,758 + AMB_RS03745 0.37 +0.6 793,967 + AMB_RS03745 0.79 -0.2 793,967 + AMB_RS03745 0.79 -0.3 793,967 + AMB_RS03745 0.79 -1.7 793,967 + AMB_RS03745 0.79 -1.5 793,967 + AMB_RS03745 0.79 -0.1 793,967 + AMB_RS03745 0.79 -1.3 793,967 + AMB_RS03745 0.79 -2.2 793,968 - AMB_RS03745 0.79 +0.1 793,968 - AMB_RS03745 0.79 -1.5 793,968 - AMB_RS03745 0.79 -0.1 793,987 + AMB_RS03745 0.83 +0.0 793,987 + AMB_RS03745 0.83 +0.6 793,987 + AMB_RS03745 0.83 -1.6 793,987 + AMB_RS03745 0.83 +1.0 793,988 - AMB_RS03745 0.84 +1.5 793,988 - AMB_RS03745 0.84 -0.1 793,988 - AMB_RS03745 0.84 +0.3 793,988 - AMB_RS03745 0.84 +0.2 793,988 - AMB_RS03745 0.84 -2.0 794,102 + +0.1 794,103 - +0.6 794,103 - -0.3 794,151 + -0.9 794,151 + -0.4 794,151 + +0.8 794,152 - -1.3 794,163 + +0.4 794,163 + -2.8 794,163 + +0.2 794,163 + -0.5 794,163 + +0.1 794,163 + -2.3 794,164 - -2.0 794,164 - -0.2 794,164 - +0.1 794,354 + AMB_RS03750 0.31 -1.5 794,355 - AMB_RS03750 0.31 -1.3 794,396 + AMB_RS03750 0.38 +0.6 794,453 + AMB_RS03750 0.46 -0.9 794,453 + AMB_RS03750 0.46 +0.0 794,453 + AMB_RS03750 0.46 -0.9 794,454 - AMB_RS03750 0.46 -0.8 794,468 + AMB_RS03750 0.49 -1.3 794,468 + AMB_RS03750 0.49 +1.5 794,468 + AMB_RS03750 0.49 +0.0 794,468 + AMB_RS03750 0.49 -0.1 794,468 + AMB_RS03750 0.49 -1.5 794,469 - AMB_RS03750 0.49 +0.5 794,469 - AMB_RS03750 0.49 +0.3 794,469 - AMB_RS03750 0.49 -1.3 794,469 - AMB_RS03750 0.49 +0.4 794,469 - AMB_RS03750 0.49 -0.2 794,469 - AMB_RS03750 0.49 +1.5 794,469 - AMB_RS03750 0.49 -2.0 794,469 - AMB_RS03750 0.49 -1.1 794,469 - AMB_RS03750 0.49 +1.5 794,469 - AMB_RS03750 0.49 -0.1 794,821 + -1.9 794,983 + AMB_RS03755 0.36 -0.2 794,983 + AMB_RS03755 0.36 -1.9 794,984 - AMB_RS03755 0.36 -0.1 795,043 + AMB_RS03755 0.48 -0.2 795,043 + AMB_RS03755 0.48 +0.0 795,043 + AMB_RS03755 0.48 -1.1 795,043 + AMB_RS03755 0.48 -0.4 795,043 + AMB_RS03755 0.48 +1.5 795,044 - AMB_RS03755 0.48 -0.9 795,044 - AMB_RS03755 0.48 -0.2 795,121 + AMB_RS03755 0.64 -0.3 795,121 + AMB_RS03755 0.64 -0.3 795,121 + AMB_RS03755 0.64 +0.4 795,121 + AMB_RS03755 0.64 -0.1 795,122 - AMB_RS03755 0.64 -0.4 795,122 - AMB_RS03755 0.64 -0.6 795,122 - AMB_RS03755 0.64 -0.1 795,122 - AMB_RS03755 0.64 -1.1 795,248 - -0.1 795,267 + -0.8 795,267 + -0.2 795,267 + -0.8 795,268 - -0.4 795,268 - +0.6 795,268 - -0.1 795,332 - -2.1 795,332 - -2.1 795,371 + -0.0 795,371 + +0.2 795,371 + -0.3 795,372 - +0.1 795,372 - -0.0 795,372 - -1.1
Or see this region's nucleotide sequence