Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03395 and AMB_RS03400 are separated by 9 nucleotides AMB_RS03400 and AMB_RS03405 are separated by 418 nucleotides AMB_RS03405 and AMB_RS03410 are separated by 75 nucleotides
AMB_RS03395: AMB_RS03395 - addiction module antidote protein, HigA family, at 712,501 to 712,788
_RS03395
AMB_RS03400: AMB_RS03400 - plasmid maintenance system killer protein, at 712,798 to 713,076
_RS03400
AMB_RS03405: AMB_RS03405 - LysR family transcriptional regulator, at 713,495 to 714,388
_RS03405
AMB_RS03410: AMB_RS03410 - thioredoxin-disulfide reductase, at 714,464 to 715,414
_RS03410
Position (kb) 713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 712.502 kb on + strand at 712.806 kb on - strand at 712.806 kb on - strand at 712.847 kb on - strand, within AMB_RS03400 at 712.847 kb on - strand, within AMB_RS03400 at 712.847 kb on - strand, within AMB_RS03400 at 712.849 kb on - strand, within AMB_RS03400 at 712.849 kb on - strand, within AMB_RS03400 at 713.037 kb on - strand, within AMB_RS03400 at 713.037 kb on - strand, within AMB_RS03400 at 713.039 kb on - strand, within AMB_RS03400 at 713.039 kb on - strand, within AMB_RS03400 at 713.088 kb on - strand at 713.104 kb on - strand at 713.168 kb on + strand at 713.169 kb on - strand at 713.298 kb on - strand at 713.393 kb on + strand at 713.402 kb on - strand at 713.468 kb on - strand at 713.583 kb on + strand at 713.620 kb on + strand, within AMB_RS03405 at 713.817 kb on + strand, within AMB_RS03405 at 713.818 kb on - strand, within AMB_RS03405 at 713.881 kb on - strand, within AMB_RS03405 at 713.904 kb on + strand, within AMB_RS03405 at 714.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 712,502 + +0.8 712,806 - -0.1 712,806 - -2.6 712,847 - AMB_RS03400 0.18 +0.0 712,847 - AMB_RS03400 0.18 -3.1 712,847 - AMB_RS03400 0.18 -1.5 712,849 - AMB_RS03400 0.18 -1.2 712,849 - AMB_RS03400 0.18 +0.3 713,037 - AMB_RS03400 0.86 +0.5 713,037 - AMB_RS03400 0.86 +0.5 713,039 - AMB_RS03400 0.86 -1.8 713,039 - AMB_RS03400 0.86 -1.5 713,088 - -1.5 713,104 - -0.4 713,168 + +0.5 713,169 - +0.5 713,298 - +1.6 713,393 + -1.1 713,402 - -2.0 713,468 - -2.0 713,583 + -1.9 713,620 + AMB_RS03405 0.14 -2.9 713,817 + AMB_RS03405 0.36 -3.2 713,818 - AMB_RS03405 0.36 +0.1 713,881 - AMB_RS03405 0.43 -2.1 713,904 + AMB_RS03405 0.46 -1.8 714,420 - -0.5
Or see this region's nucleotide sequence