Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03240 and AMB_RS03245 are separated by 193 nucleotides AMB_RS03245 and AMB_RS03250 are separated by 154 nucleotides AMB_RS03250 and AMB_RS03255 are separated by 66 nucleotides
AMB_RS03240: AMB_RS03240 - DNA-binding response regulator, at 677,510 to 678,226
_RS03240
AMB_RS03245: AMB_RS03245 - 2-polyprenylphenol 6-hydroxylase, at 678,420 to 679,961
_RS03245
AMB_RS03250: AMB_RS03250 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 680,116 to 680,874
_RS03250
AMB_RS03255: AMB_RS03255 - bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase, at 680,941 to 681,780
_RS03255
Position (kb) 678
679
680 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 677.457 kb on + strand at 677.457 kb on + strand at 677.457 kb on + strand at 677.458 kb on - strand at 677.465 kb on + strand at 677.465 kb on + strand at 677.471 kb on + strand at 677.472 kb on - strand at 677.481 kb on + strand at 677.481 kb on + strand at 677.481 kb on + strand at 677.482 kb on - strand at 677.506 kb on + strand at 677.506 kb on + strand at 677.506 kb on + strand at 677.506 kb on + strand at 677.507 kb on - strand at 677.507 kb on - strand at 677.507 kb on - strand at 677.507 kb on - strand at 677.636 kb on + strand, within AMB_RS03240 at 677.636 kb on + strand, within AMB_RS03240 at 677.637 kb on - strand, within AMB_RS03240 at 677.642 kb on + strand, within AMB_RS03240 at 677.687 kb on + strand, within AMB_RS03240 at 677.801 kb on + strand, within AMB_RS03240 at 677.802 kb on - strand, within AMB_RS03240 at 677.802 kb on - strand, within AMB_RS03240 at 678.033 kb on + strand, within AMB_RS03240 at 678.034 kb on - strand, within AMB_RS03240 at 678.034 kb on - strand, within AMB_RS03240 at 678.044 kb on + strand, within AMB_RS03240 at 678.044 kb on + strand, within AMB_RS03240 at 678.045 kb on - strand, within AMB_RS03240 at 678.045 kb on - strand, within AMB_RS03240 at 678.137 kb on + strand, within AMB_RS03240 at 678.137 kb on + strand, within AMB_RS03240 at 678.164 kb on + strand at 678.164 kb on + strand at 678.164 kb on + strand at 678.165 kb on - strand at 678.165 kb on - strand at 678.346 kb on - strand at 678.346 kb on - strand at 678.349 kb on + strand at 678.352 kb on - strand at 678.421 kb on + strand at 680.120 kb on - strand at 680.120 kb on - strand at 680.120 kb on - strand at 680.120 kb on - strand at 680.325 kb on - strand, within AMB_RS03250 at 680.928 kb on + strand at 680.928 kb on + strand at 680.928 kb on + strand at 680.928 kb on + strand at 680.928 kb on + strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.930 kb on + strand at 680.930 kb on + strand at 680.930 kb on + strand at 680.931 kb on - strand at 680.931 kb on - strand at 680.931 kb on - strand at 680.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 677,457 + -1.1 677,457 + -0.3 677,457 + +0.1 677,458 - -0.2 677,465 + +0.4 677,465 + -0.9 677,471 + +0.2 677,472 - -0.8 677,481 + -1.1 677,481 + -1.1 677,481 + -0.1 677,482 - -0.1 677,506 + -0.2 677,506 + -1.8 677,506 + +0.7 677,506 + -0.5 677,507 - +0.5 677,507 - -0.1 677,507 - -0.5 677,507 - +0.1 677,636 + AMB_RS03240 0.18 -0.5 677,636 + AMB_RS03240 0.18 +0.0 677,637 - AMB_RS03240 0.18 -0.1 677,642 + AMB_RS03240 0.18 -0.4 677,687 + AMB_RS03240 0.25 +1.5 677,801 + AMB_RS03240 0.41 +0.1 677,802 - AMB_RS03240 0.41 -1.5 677,802 - AMB_RS03240 0.41 -0.2 678,033 + AMB_RS03240 0.73 -1.3 678,034 - AMB_RS03240 0.73 -0.1 678,034 - AMB_RS03240 0.73 -0.1 678,044 + AMB_RS03240 0.74 -0.5 678,044 + AMB_RS03240 0.74 -0.9 678,045 - AMB_RS03240 0.75 -0.5 678,045 - AMB_RS03240 0.75 -0.1 678,137 + AMB_RS03240 0.87 +0.1 678,137 + AMB_RS03240 0.87 +0.7 678,164 + -0.2 678,164 + -0.6 678,164 + +0.1 678,165 - +0.0 678,165 - -0.3 678,346 - -0.2 678,346 - -1.8 678,349 + -0.2 678,352 - +0.5 678,421 + +0.1 680,120 - -3.1 680,120 - -2.1 680,120 - -3.6 680,120 - -2.5 680,325 - AMB_RS03250 0.28 -2.5 680,928 + -1.2 680,928 + -0.5 680,928 + -0.9 680,928 + -1.5 680,928 + -1.1 680,929 - -0.1 680,929 - +0.5 680,929 - -0.2 680,929 - +0.6 680,929 - -0.8 680,929 - -0.2 680,929 - -0.1 680,929 - -1.1 680,930 + +0.0 680,930 + +0.0 680,930 + -1.8 680,931 - +0.5 680,931 - -0.8 680,931 - +1.0 680,931 - -0.8
Or see this region's nucleotide sequence