Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03100 and AMB_RS03105 are separated by 24 nucleotides AMB_RS03105 and AMB_RS03110 are separated by 116 nucleotides AMB_RS03110 and AMB_RS03115 are separated by 34 nucleotides AMB_RS03115 and AMB_RS03120 overlap by 1 nucleotides
AMB_RS03100: AMB_RS03100 - isoprenylcysteine carboxylmethyltransferase family protein, at 647,837 to 648,454
_RS03100
AMB_RS03105: AMB_RS03105 - hypothetical protein, at 648,479 to 648,991
_RS03105
AMB_RS03110: AMB_RS03110 - membrane protein, at 649,108 to 650,244
_RS03110
AMB_RS03115: AMB_RS03115 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 650,279 to 651,073
_RS03115
AMB_RS03120: AMB_RS03120 - phosphatidylserine decarboxylase family protein, at 651,073 to 651,795
_RS03120
Position (kb) 649
650
651 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 648.206 kb on + strand, within AMB_RS03100 at 648.207 kb on - strand, within AMB_RS03100 at 648.207 kb on - strand, within AMB_RS03100 at 648.284 kb on + strand, within AMB_RS03100 at 648.285 kb on - strand, within AMB_RS03100 at 648.302 kb on + strand, within AMB_RS03100 at 648.302 kb on + strand, within AMB_RS03100 at 648.302 kb on + strand, within AMB_RS03100 at 648.303 kb on - strand, within AMB_RS03100 at 648.303 kb on - strand, within AMB_RS03100 at 648.303 kb on - strand, within AMB_RS03100 at 648.303 kb on - strand, within AMB_RS03100 at 648.462 kb on + strand at 648.463 kb on - strand at 648.463 kb on - strand at 648.732 kb on + strand, within AMB_RS03105 at 648.733 kb on - strand, within AMB_RS03105 at 648.733 kb on - strand, within AMB_RS03105 at 648.733 kb on - strand, within AMB_RS03105 at 648.733 kb on - strand, within AMB_RS03105 at 648.852 kb on + strand, within AMB_RS03105 at 648.852 kb on + strand, within AMB_RS03105 at 648.904 kb on - strand, within AMB_RS03105 at 649.023 kb on + strand at 649.024 kb on - strand at 649.024 kb on - strand at 649.024 kb on - strand at 649.027 kb on - strand at 649.671 kb on - strand, within AMB_RS03110 at 649.720 kb on - strand, within AMB_RS03110 at 649.722 kb on - strand, within AMB_RS03110 at 650.063 kb on + strand, within AMB_RS03110 at 650.064 kb on - strand, within AMB_RS03110 at 650.146 kb on + strand at 650.193 kb on - strand at 650.196 kb on + strand at 650.196 kb on + strand at 650.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 648,206 + AMB_RS03100 0.60 +0.4 648,207 - AMB_RS03100 0.60 +1.0 648,207 - AMB_RS03100 0.60 -0.8 648,284 + AMB_RS03100 0.72 -1.8 648,285 - AMB_RS03100 0.72 -2.2 648,302 + AMB_RS03100 0.75 -0.9 648,302 + AMB_RS03100 0.75 -0.4 648,302 + AMB_RS03100 0.75 +0.0 648,303 - AMB_RS03100 0.75 -1.8 648,303 - AMB_RS03100 0.75 -0.4 648,303 - AMB_RS03100 0.75 -2.8 648,303 - AMB_RS03100 0.75 -2.6 648,462 + +0.5 648,463 - -0.3 648,463 - -1.1 648,732 + AMB_RS03105 0.49 -2.7 648,733 - AMB_RS03105 0.50 +0.5 648,733 - AMB_RS03105 0.50 +0.3 648,733 - AMB_RS03105 0.50 -2.3 648,733 - AMB_RS03105 0.50 +0.9 648,852 + AMB_RS03105 0.73 -3.0 648,852 + AMB_RS03105 0.73 -0.2 648,904 - AMB_RS03105 0.83 -0.3 649,023 + -2.1 649,024 - -0.3 649,024 - +1.5 649,024 - -1.7 649,027 - +0.0 649,671 - AMB_RS03110 0.50 -5.2 649,720 - AMB_RS03110 0.54 -1.1 649,722 - AMB_RS03110 0.54 -1.7 650,063 + AMB_RS03110 0.84 -2.2 650,064 - AMB_RS03110 0.84 -3.2 650,146 + -2.1 650,193 - -3.1 650,196 + -1.3 650,196 + -5.7 650,196 + -0.8
Or see this region's nucleotide sequence