Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02810 and AMB_RS02815 are separated by 191 nucleotides AMB_RS02815 and AMB_RS02820 are separated by 64 nucleotides AMB_RS02820 and AMB_RS02825 are separated by 130 nucleotides AMB_RS02825 and AMB_RS02830 are separated by 81 nucleotides
AMB_RS02810: AMB_RS02810 - hypothetical protein, at 591,239 to 591,535
_RS02810
AMB_RS02815: AMB_RS02815 - hypothetical protein, at 591,727 to 592,230
_RS02815
AMB_RS02820: AMB_RS02820 - pyrroline-5-carboxylate reductase, at 592,295 to 593,086
_RS02820
AMB_RS02825: AMB_RS02825 - phasin, at 593,217 to 593,654
_RS02825
AMB_RS02830: AMB_RS02830 - chromosome partitioning protein ParA, at 593,736 to 594,359
_RS02830
Position (kb) 592
593
594 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 591.578 kb on - strand at 591.578 kb on - strand at 591.585 kb on + strand at 591.585 kb on + strand at 591.586 kb on - strand at 591.589 kb on + strand at 591.589 kb on + strand at 591.590 kb on - strand at 591.745 kb on + strand at 591.746 kb on - strand at 591.871 kb on + strand, within AMB_RS02815 at 591.871 kb on + strand, within AMB_RS02815 at 591.872 kb on - strand, within AMB_RS02815 at 591.872 kb on - strand, within AMB_RS02815 at 591.872 kb on - strand, within AMB_RS02815 at 591.880 kb on + strand, within AMB_RS02815 at 591.880 kb on + strand, within AMB_RS02815 at 591.881 kb on - strand, within AMB_RS02815 at 591.881 kb on - strand, within AMB_RS02815 at 591.900 kb on + strand, within AMB_RS02815 at 591.901 kb on - strand, within AMB_RS02815 at 591.901 kb on - strand, within AMB_RS02815 at 592.141 kb on + strand, within AMB_RS02815 at 592.141 kb on + strand, within AMB_RS02815 at 592.142 kb on - strand, within AMB_RS02815 at 593.084 kb on + strand at 593.106 kb on - strand at 593.109 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.120 kb on + strand at 593.121 kb on - strand at 593.121 kb on - strand at 593.121 kb on - strand at 593.121 kb on - strand at 593.121 kb on - strand at 593.124 kb on + strand at 593.124 kb on + strand at 593.136 kb on + strand at 593.137 kb on - strand at 593.172 kb on - strand at 593.191 kb on + strand at 593.191 kb on + strand at 593.191 kb on + strand at 593.191 kb on + strand at 593.192 kb on - strand at 593.192 kb on - strand at 593.192 kb on - strand at 593.192 kb on - strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.213 kb on + strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.214 kb on - strand at 593.265 kb on + strand, within AMB_RS02825 at 593.265 kb on + strand, within AMB_RS02825 at 593.266 kb on - strand, within AMB_RS02825 at 593.266 kb on - strand, within AMB_RS02825 at 593.266 kb on - strand, within AMB_RS02825 at 593.652 kb on + strand at 593.652 kb on + strand at 593.652 kb on + strand at 593.653 kb on - strand at 593.653 kb on - strand at 593.653 kb on - strand at 593.737 kb on + strand at 593.737 kb on + strand at 593.738 kb on - strand at 593.738 kb on - strand at 593.782 kb on + strand at 593.783 kb on - strand at 593.783 kb on - strand at 593.783 kb on - strand at 593.783 kb on - strand at 593.843 kb on + strand, within AMB_RS02830 at 593.844 kb on - strand, within AMB_RS02830 at 593.845 kb on + strand, within AMB_RS02830 at 593.845 kb on + strand, within AMB_RS02830 at 593.845 kb on + strand, within AMB_RS02830 at 593.845 kb on + strand, within AMB_RS02830 at 593.846 kb on - strand, within AMB_RS02830 at 593.953 kb on - strand, within AMB_RS02830 at 594.075 kb on - strand, within AMB_RS02830
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 591,578 - -0.3 591,578 - -0.4 591,585 + -2.7 591,585 + -0.8 591,586 - -1.8 591,589 + +0.3 591,589 + -0.8 591,590 - -0.1 591,745 + -1.2 591,746 - -2.3 591,871 + AMB_RS02815 0.29 +0.3 591,871 + AMB_RS02815 0.29 -2.3 591,872 - AMB_RS02815 0.29 -0.7 591,872 - AMB_RS02815 0.29 -3.9 591,872 - AMB_RS02815 0.29 +0.6 591,880 + AMB_RS02815 0.30 -2.0 591,880 + AMB_RS02815 0.30 +0.5 591,881 - AMB_RS02815 0.31 -2.2 591,881 - AMB_RS02815 0.31 +0.2 591,900 + AMB_RS02815 0.34 -0.8 591,901 - AMB_RS02815 0.35 -0.6 591,901 - AMB_RS02815 0.35 -2.0 592,141 + AMB_RS02815 0.82 -0.1 592,141 + AMB_RS02815 0.82 -0.7 592,142 - AMB_RS02815 0.82 +0.1 593,084 + -0.0 593,106 - +1.5 593,109 + +0.2 593,120 + +0.1 593,120 + -0.1 593,120 + +0.9 593,120 + -0.7 593,120 + -0.2 593,120 + +0.7 593,120 + +0.2 593,120 + -1.1 593,120 + -3.1 593,120 + -0.5 593,121 - +0.1 593,121 - -0.4 593,121 - +0.0 593,121 - +0.8 593,121 - +0.0 593,124 + +0.1 593,124 + +1.5 593,136 + +0.2 593,137 - +1.5 593,172 - -0.2 593,191 + -0.8 593,191 + +0.2 593,191 + -1.8 593,191 + -0.7 593,192 - -0.3 593,192 - +0.7 593,192 - +0.3 593,192 - +0.7 593,213 + -1.3 593,213 + -0.1 593,213 + +0.1 593,213 + +0.3 593,213 + -0.1 593,213 + -1.5 593,213 + +0.5 593,213 + +0.5 593,213 + +0.5 593,214 - +0.8 593,214 - +0.4 593,214 - -1.1 593,214 - +0.4 593,214 - -2.2 593,214 - +0.3 593,214 - +0.3 593,214 - -0.5 593,214 - -1.1 593,265 + AMB_RS02825 0.11 -1.1 593,265 + AMB_RS02825 0.11 -1.5 593,266 - AMB_RS02825 0.11 -1.8 593,266 - AMB_RS02825 0.11 +0.2 593,266 - AMB_RS02825 0.11 -1.5 593,652 + +0.1 593,652 + -0.0 593,652 + -0.8 593,653 - +1.5 593,653 - +1.5 593,653 - -1.1 593,737 + -1.3 593,737 + -0.8 593,738 - +0.2 593,738 - -2.7 593,782 + +0.2 593,783 - -1.5 593,783 - +1.5 593,783 - -0.1 593,783 - -0.0 593,843 + AMB_RS02830 0.17 -3.4 593,844 - AMB_RS02830 0.17 +0.2 593,845 + AMB_RS02830 0.17 +1.5 593,845 + AMB_RS02830 0.17 +0.5 593,845 + AMB_RS02830 0.17 +0.3 593,845 + AMB_RS02830 0.17 -0.5 593,846 - AMB_RS02830 0.18 +0.1 593,953 - AMB_RS02830 0.35 -0.1 594,075 - AMB_RS02830 0.54 +0.4
Or see this region's nucleotide sequence