Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23130 and AMB_RS02385 are separated by 11 nucleotides AMB_RS02385 and AMB_RS02390 are separated by 62 nucleotides AMB_RS02390 and AMB_RS02395 are separated by 113 nucleotides AMB_RS02395 and AMB_RS02400 are separated by 145 nucleotides
AMB_RS23130: AMB_RS23130 - regulatory protein GemA, at 499,658 to 500,452
_RS23130
AMB_RS02385: AMB_RS02385 - zinc ribbon domain-containing protein, at 500,464 to 500,811
_RS02385
AMB_RS02390: AMB_RS02390 - hypothetical protein, at 500,874 to 501,251
_RS02390
AMB_RS02395: AMB_RS02395 - hypothetical protein, at 501,365 to 501,817
_RS02395
AMB_RS02400: AMB_RS02400 - hypothetical protein, at 501,963 to 502,511
_RS02400
Position (kb) 500
501
502 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 500.060 kb on + strand, within AMB_RS23130 at 500.255 kb on + strand, within AMB_RS23130 at 500.255 kb on + strand, within AMB_RS23130 at 500.255 kb on + strand, within AMB_RS23130 at 500.255 kb on + strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.256 kb on - strand, within AMB_RS23130 at 500.480 kb on + strand at 500.610 kb on + strand, within AMB_RS02385 at 500.610 kb on + strand, within AMB_RS02385 at 500.611 kb on - strand, within AMB_RS02385 at 500.611 kb on - strand, within AMB_RS02385 at 500.611 kb on - strand, within AMB_RS02385 at 500.611 kb on - strand, within AMB_RS02385 at 500.611 kb on - strand, within AMB_RS02385 at 500.611 kb on - strand, within AMB_RS02385 at 500.711 kb on + strand, within AMB_RS02385 at 500.711 kb on + strand, within AMB_RS02385 at 500.712 kb on - strand, within AMB_RS02385 at 500.712 kb on - strand, within AMB_RS02385 at 500.712 kb on - strand, within AMB_RS02385 at 500.712 kb on - strand, within AMB_RS02385 at 500.712 kb on - strand, within AMB_RS02385 at 500.979 kb on + strand, within AMB_RS02390 at 500.979 kb on + strand, within AMB_RS02390 at 500.979 kb on + strand, within AMB_RS02390 at 500.979 kb on + strand, within AMB_RS02390 at 500.979 kb on + strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 500.980 kb on - strand, within AMB_RS02390 at 501.013 kb on + strand, within AMB_RS02390 at 501.143 kb on + strand, within AMB_RS02390 at 501.186 kb on + strand, within AMB_RS02390 at 501.186 kb on + strand, within AMB_RS02390 at 501.186 kb on + strand, within AMB_RS02390 at 501.187 kb on - strand, within AMB_RS02390 at 501.187 kb on - strand, within AMB_RS02390 at 501.311 kb on + strand at 501.311 kb on + strand at 501.311 kb on + strand at 501.311 kb on + strand at 501.311 kb on + strand at 501.311 kb on + strand at 501.312 kb on - strand at 501.312 kb on - strand at 501.312 kb on - strand at 501.328 kb on + strand at 501.328 kb on + strand at 501.540 kb on - strand, within AMB_RS02395 at 501.540 kb on - strand, within AMB_RS02395 at 501.540 kb on - strand, within AMB_RS02395 at 501.540 kb on - strand, within AMB_RS02395 at 501.540 kb on - strand, within AMB_RS02395 at 501.617 kb on + strand, within AMB_RS02395 at 501.631 kb on - strand, within AMB_RS02395
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 500,060 + AMB_RS23130 0.51 -0.8 500,255 + AMB_RS23130 0.75 +0.1 500,255 + AMB_RS23130 0.75 +0.3 500,255 + AMB_RS23130 0.75 -2.1 500,255 + AMB_RS23130 0.75 -0.1 500,256 - AMB_RS23130 0.75 +0.2 500,256 - AMB_RS23130 0.75 -0.7 500,256 - AMB_RS23130 0.75 +0.8 500,256 - AMB_RS23130 0.75 -0.1 500,256 - AMB_RS23130 0.75 -0.9 500,256 - AMB_RS23130 0.75 +0.5 500,256 - AMB_RS23130 0.75 -1.1 500,256 - AMB_RS23130 0.75 -2.6 500,480 + +0.3 500,610 + AMB_RS02385 0.42 -0.9 500,610 + AMB_RS02385 0.42 -0.5 500,611 - AMB_RS02385 0.42 -1.1 500,611 - AMB_RS02385 0.42 +0.3 500,611 - AMB_RS02385 0.42 -0.3 500,611 - AMB_RS02385 0.42 -1.8 500,611 - AMB_RS02385 0.42 -0.0 500,611 - AMB_RS02385 0.42 -1.1 500,711 + AMB_RS02385 0.71 +0.1 500,711 + AMB_RS02385 0.71 -1.5 500,712 - AMB_RS02385 0.71 -1.5 500,712 - AMB_RS02385 0.71 -0.1 500,712 - AMB_RS02385 0.71 -0.5 500,712 - AMB_RS02385 0.71 -1.3 500,712 - AMB_RS02385 0.71 -0.8 500,979 + AMB_RS02390 0.28 -0.2 500,979 + AMB_RS02390 0.28 +0.2 500,979 + AMB_RS02390 0.28 +0.5 500,979 + AMB_RS02390 0.28 -1.1 500,979 + AMB_RS02390 0.28 -1.1 500,980 - AMB_RS02390 0.28 -0.5 500,980 - AMB_RS02390 0.28 -1.1 500,980 - AMB_RS02390 0.28 +0.1 500,980 - AMB_RS02390 0.28 -1.0 500,980 - AMB_RS02390 0.28 -0.1 500,980 - AMB_RS02390 0.28 -1.3 500,980 - AMB_RS02390 0.28 -1.5 501,013 + AMB_RS02390 0.37 +1.5 501,143 + AMB_RS02390 0.71 +0.4 501,186 + AMB_RS02390 0.83 -3.1 501,186 + AMB_RS02390 0.83 -1.1 501,186 + AMB_RS02390 0.83 -0.8 501,187 - AMB_RS02390 0.83 -2.3 501,187 - AMB_RS02390 0.83 +1.5 501,311 + -0.5 501,311 + +0.7 501,311 + +1.5 501,311 + +0.9 501,311 + +1.5 501,311 + -1.5 501,312 - -0.3 501,312 - -0.4 501,312 - -1.5 501,328 + -0.5 501,328 + +0.2 501,540 - AMB_RS02395 0.39 -0.8 501,540 - AMB_RS02395 0.39 -1.1 501,540 - AMB_RS02395 0.39 +0.1 501,540 - AMB_RS02395 0.39 +0.1 501,540 - AMB_RS02395 0.39 -0.3 501,617 + AMB_RS02395 0.56 -0.1 501,631 - AMB_RS02395 0.59 +0.2
Or see this region's nucleotide sequence