Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23120 and AMB_RS02295 overlap by 16 nucleotides AMB_RS02295 and AMB_RS02300 are separated by 176 nucleotides AMB_RS02300 and AMB_RS02305 overlap by 8 nucleotides AMB_RS02305 and AMB_RS02310 are separated by 67 nucleotides AMB_RS02310 and AMB_RS02315 overlap by 1 nucleotides
AMB_RS23120: AMB_RS23120 - helix-turn-helix domain-containing protein, at 487,708 to 488,748
_RS23120
AMB_RS02295: AMB_RS02295 - XRE family transcriptional regulator, at 488,733 to 488,999
_RS02295
AMB_RS02300: AMB_RS02300 - hypothetical protein, at 489,176 to 489,676
_RS02300
AMB_RS02305: AMB_RS02305 - transposase, at 489,669 to 491,870
_RS02305
AMB_RS02310: AMB_RS02310 - ATP-binding protein, at 491,938 to 492,681
_RS02310
AMB_RS02315: AMB_RS02315 - hypothetical protein, at 492,681 to 493,325
_RS02315
Position (kb) 489
490
491
492 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 488.781 kb on + strand, within AMB_RS02295 at 488.782 kb on - strand, within AMB_RS02295 at 488.967 kb on - strand, within AMB_RS02295 at 488.967 kb on - strand, within AMB_RS02295 at 489.017 kb on - strand at 489.829 kb on - strand at 490.246 kb on - strand, within AMB_RS02305 at 490.246 kb on - strand, within AMB_RS02305 at 490.246 kb on - strand, within AMB_RS02305 at 490.246 kb on - strand, within AMB_RS02305 at 490.246 kb on - strand, within AMB_RS02305 at 490.452 kb on + strand, within AMB_RS02305 at 490.453 kb on - strand, within AMB_RS02305 at 490.453 kb on - strand, within AMB_RS02305 at 490.749 kb on + strand, within AMB_RS02305 at 490.750 kb on - strand, within AMB_RS02305 at 490.750 kb on - strand, within AMB_RS02305 at 490.750 kb on - strand, within AMB_RS02305 at 490.750 kb on - strand, within AMB_RS02305 at 490.750 kb on - strand, within AMB_RS02305 at 490.893 kb on + strand, within AMB_RS02305 at 490.894 kb on - strand, within AMB_RS02305 at 491.302 kb on - strand, within AMB_RS02305 at 491.302 kb on - strand, within AMB_RS02305 at 491.302 kb on - strand, within AMB_RS02305 at 491.302 kb on - strand, within AMB_RS02305 at 491.326 kb on - strand, within AMB_RS02305 at 491.812 kb on - strand at 491.923 kb on - strand at 491.923 kb on - strand at 492.055 kb on + strand, within AMB_RS02310 at 492.055 kb on + strand, within AMB_RS02310 at 492.055 kb on + strand, within AMB_RS02310 at 492.056 kb on - strand, within AMB_RS02310 at 492.056 kb on - strand, within AMB_RS02310 at 492.097 kb on + strand, within AMB_RS02310 at 492.098 kb on - strand, within AMB_RS02310 at 492.098 kb on - strand, within AMB_RS02310 at 492.098 kb on - strand, within AMB_RS02310 at 492.098 kb on - strand, within AMB_RS02310 at 492.098 kb on - strand, within AMB_RS02310 at 492.121 kb on + strand, within AMB_RS02310 at 492.122 kb on - strand, within AMB_RS02310 at 492.325 kb on + strand, within AMB_RS02310 at 492.325 kb on + strand, within AMB_RS02310 at 492.325 kb on + strand, within AMB_RS02310 at 492.325 kb on + strand, within AMB_RS02310 at 492.325 kb on + strand, within AMB_RS02310 at 492.325 kb on + strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.326 kb on - strand, within AMB_RS02310 at 492.709 kb on + strand at 492.710 kb on - strand at 492.837 kb on + strand, within AMB_RS02315 at 492.838 kb on - strand, within AMB_RS02315 at 492.864 kb on + strand, within AMB_RS02315 at 492.865 kb on - strand, within AMB_RS02315
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 488,781 + AMB_RS02295 0.18 -0.1 488,782 - AMB_RS02295 0.18 -0.0 488,967 - AMB_RS02295 0.88 +0.3 488,967 - AMB_RS02295 0.88 -0.0 489,017 - -0.0 489,829 - +0.1 490,246 - AMB_RS02305 0.26 +0.2 490,246 - AMB_RS02305 0.26 -1.8 490,246 - AMB_RS02305 0.26 -0.1 490,246 - AMB_RS02305 0.26 -1.3 490,246 - AMB_RS02305 0.26 -2.1 490,452 + AMB_RS02305 0.36 -1.1 490,453 - AMB_RS02305 0.36 -1.6 490,453 - AMB_RS02305 0.36 +0.3 490,749 + AMB_RS02305 0.49 -1.8 490,750 - AMB_RS02305 0.49 -0.2 490,750 - AMB_RS02305 0.49 -2.4 490,750 - AMB_RS02305 0.49 -0.4 490,750 - AMB_RS02305 0.49 -1.1 490,750 - AMB_RS02305 0.49 -1.1 490,893 + AMB_RS02305 0.56 -1.8 490,894 - AMB_RS02305 0.56 -1.5 491,302 - AMB_RS02305 0.74 -1.3 491,302 - AMB_RS02305 0.74 -1.9 491,302 - AMB_RS02305 0.74 +0.3 491,302 - AMB_RS02305 0.74 +0.2 491,326 - AMB_RS02305 0.75 -0.3 491,812 - -1.3 491,923 - -1.5 491,923 - +0.1 492,055 + AMB_RS02310 0.16 +0.2 492,055 + AMB_RS02310 0.16 -0.4 492,055 + AMB_RS02310 0.16 +1.5 492,056 - AMB_RS02310 0.16 -0.0 492,056 - AMB_RS02310 0.16 -0.1 492,097 + AMB_RS02310 0.21 +0.5 492,098 - AMB_RS02310 0.22 +0.5 492,098 - AMB_RS02310 0.22 -0.1 492,098 - AMB_RS02310 0.22 +0.1 492,098 - AMB_RS02310 0.22 +0.2 492,098 - AMB_RS02310 0.22 +0.4 492,121 + AMB_RS02310 0.25 +0.9 492,122 - AMB_RS02310 0.25 +0.2 492,325 + AMB_RS02310 0.52 -0.5 492,325 + AMB_RS02310 0.52 -1.3 492,325 + AMB_RS02310 0.52 -1.1 492,325 + AMB_RS02310 0.52 -2.9 492,325 + AMB_RS02310 0.52 -2.3 492,325 + AMB_RS02310 0.52 +0.6 492,326 - AMB_RS02310 0.52 -0.2 492,326 - AMB_RS02310 0.52 -2.5 492,326 - AMB_RS02310 0.52 -2.1 492,326 - AMB_RS02310 0.52 -1.1 492,326 - AMB_RS02310 0.52 -2.2 492,326 - AMB_RS02310 0.52 +0.4 492,326 - AMB_RS02310 0.52 +0.0 492,326 - AMB_RS02310 0.52 -0.8 492,709 + +0.3 492,710 - -0.7 492,837 + AMB_RS02315 0.24 +0.1 492,838 - AMB_RS02315 0.24 +0.1 492,864 + AMB_RS02315 0.28 +0.9 492,865 - AMB_RS02315 0.29 -0.5
Or see this region's nucleotide sequence