Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02225 and AMB_RS02230 are separated by 85 nucleotides AMB_RS02230 and AMB_RS02235 overlap by 4 nucleotides AMB_RS02235 and AMB_RS02240 are separated by 277 nucleotides
AMB_RS02225: AMB_RS02225 - methionine synthase, at 469,104 to 472,577
_RS02225
AMB_RS02230: AMB_RS02230 - methylenetetrahydrofolate reductase, at 472,663 to 473,559
_RS02230
AMB_RS02235: AMB_RS02235 - methyltransferase domain-containing protein, at 473,556 to 474,515
_RS02235
AMB_RS02240: AMB_RS02240 - surface lipoprotein, at 474,793 to 475,605
_RS02240
Position (kb) 473
474
475 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 472.665 kb on - strand at 473.840 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 473.845 kb on - strand, within AMB_RS02235 at 474.173 kb on - strand, within AMB_RS02235 at 474.314 kb on - strand, within AMB_RS02235 at 474.314 kb on - strand, within AMB_RS02235 at 474.314 kb on - strand, within AMB_RS02235 at 474.314 kb on - strand, within AMB_RS02235 at 474.615 kb on + strand at 474.696 kb on + strand at 474.769 kb on + strand at 474.769 kb on + strand at 474.774 kb on + strand at 475.465 kb on + strand, within AMB_RS02240 at 475.465 kb on + strand, within AMB_RS02240 at 475.465 kb on + strand, within AMB_RS02240
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 472,665 - +0.7 473,840 - AMB_RS02235 0.30 -2.2 473,845 - AMB_RS02235 0.30 +0.6 473,845 - AMB_RS02235 0.30 -3.4 473,845 - AMB_RS02235 0.30 -2.9 473,845 - AMB_RS02235 0.30 -1.9 473,845 - AMB_RS02235 0.30 -2.1 473,845 - AMB_RS02235 0.30 -0.1 473,845 - AMB_RS02235 0.30 -2.4 474,173 - AMB_RS02235 0.64 -1.3 474,314 - AMB_RS02235 0.79 -1.3 474,314 - AMB_RS02235 0.79 -3.0 474,314 - AMB_RS02235 0.79 -2.2 474,314 - AMB_RS02235 0.79 -1.0 474,615 + +0.1 474,696 + -0.5 474,769 + -0.5 474,769 + -1.3 474,774 + +1.5 475,465 + AMB_RS02240 0.83 -0.5 475,465 + AMB_RS02240 0.83 +0.1 475,465 + AMB_RS02240 0.83 -1.5
Or see this region's nucleotide sequence