Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01995 and AMB_RS02000 are separated by 100 nucleotides AMB_RS02000 and AMB_RS02005 overlap by 4 nucleotides AMB_RS02005 and AMB_RS02010 are separated by 62 nucleotides AMB_RS02010 and AMB_RS02015 are separated by 2 nucleotides
AMB_RS01995: AMB_RS01995 - DUF1833 domain-containing protein, at 420,223 to 420,747
_RS01995
AMB_RS02000: AMB_RS02000 - hypothetical protein, at 420,848 to 421,243
_RS02000
AMB_RS02005: AMB_RS02005 - hypothetical protein, at 421,240 to 423,567
_RS02005
AMB_RS02010: AMB_RS02010 - structural protein, at 423,630 to 424,067
_RS02010
AMB_RS02015: AMB_RS02015 - hypothetical protein, at 424,070 to 424,666
_RS02015
Position (kb) 421
422
423
424 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 420.572 kb on - strand, within AMB_RS01995 at 420.572 kb on - strand, within AMB_RS01995 at 420.572 kb on - strand, within AMB_RS01995 at 420.712 kb on + strand at 420.712 kb on + strand at 420.712 kb on + strand at 420.713 kb on - strand at 420.713 kb on - strand at 420.774 kb on - strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.788 kb on + strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.789 kb on - strand at 420.866 kb on + strand at 420.868 kb on + strand at 420.868 kb on + strand at 421.253 kb on + strand at 421.253 kb on + strand at 421.253 kb on + strand at 421.253 kb on + strand at 421.253 kb on + strand at 421.253 kb on + strand at 421.254 kb on - strand at 421.254 kb on - strand at 421.254 kb on - strand at 421.254 kb on - strand at 421.254 kb on - strand at 421.254 kb on - strand at 421.254 kb on - strand at 421.257 kb on + strand at 421.257 kb on + strand at 421.257 kb on + strand at 421.257 kb on + strand at 421.258 kb on - strand at 421.258 kb on - strand at 421.258 kb on - strand at 421.258 kb on - strand at 421.258 kb on - strand at 421.420 kb on + strand at 421.420 kb on + strand at 421.420 kb on + strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.556 kb on - strand, within AMB_RS02005 at 421.556 kb on - strand, within AMB_RS02005 at 421.556 kb on - strand, within AMB_RS02005 at 421.588 kb on + strand, within AMB_RS02005 at 421.588 kb on + strand, within AMB_RS02005 at 421.588 kb on + strand, within AMB_RS02005 at 421.589 kb on - strand, within AMB_RS02005 at 421.589 kb on - strand, within AMB_RS02005 at 421.589 kb on - strand, within AMB_RS02005 at 421.589 kb on - strand, within AMB_RS02005 at 421.590 kb on + strand, within AMB_RS02005 at 421.659 kb on + strand, within AMB_RS02005 at 421.660 kb on - strand, within AMB_RS02005 at 421.840 kb on + strand, within AMB_RS02005 at 421.840 kb on + strand, within AMB_RS02005 at 421.849 kb on + strand, within AMB_RS02005 at 421.849 kb on + strand, within AMB_RS02005 at 421.867 kb on + strand, within AMB_RS02005 at 421.867 kb on + strand, within AMB_RS02005 at 421.867 kb on + strand, within AMB_RS02005 at 421.873 kb on + strand, within AMB_RS02005 at 421.873 kb on + strand, within AMB_RS02005 at 422.083 kb on + strand, within AMB_RS02005 at 422.083 kb on + strand, within AMB_RS02005 at 422.084 kb on - strand, within AMB_RS02005 at 422.085 kb on + strand, within AMB_RS02005 at 422.085 kb on + strand, within AMB_RS02005 at 422.085 kb on + strand, within AMB_RS02005 at 422.085 kb on + strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.086 kb on - strand, within AMB_RS02005 at 422.164 kb on + strand, within AMB_RS02005 at 422.164 kb on + strand, within AMB_RS02005 at 422.164 kb on + strand, within AMB_RS02005 at 422.164 kb on + strand, within AMB_RS02005 at 422.250 kb on + strand, within AMB_RS02005 at 422.250 kb on + strand, within AMB_RS02005 at 422.250 kb on + strand, within AMB_RS02005 at 422.250 kb on + strand, within AMB_RS02005 at 422.250 kb on + strand, within AMB_RS02005 at 422.251 kb on - strand, within AMB_RS02005 at 422.251 kb on - strand, within AMB_RS02005 at 422.251 kb on - strand, within AMB_RS02005 at 422.251 kb on - strand, within AMB_RS02005 at 422.251 kb on - strand, within AMB_RS02005 at 422.287 kb on - strand, within AMB_RS02005 at 422.432 kb on - strand, within AMB_RS02005 at 422.432 kb on - strand, within AMB_RS02005 at 422.638 kb on + strand, within AMB_RS02005 at 422.638 kb on + strand, within AMB_RS02005 at 422.733 kb on - strand, within AMB_RS02005 at 422.905 kb on + strand, within AMB_RS02005 at 422.905 kb on + strand, within AMB_RS02005 at 422.905 kb on + strand, within AMB_RS02005 at 422.905 kb on + strand, within AMB_RS02005 at 422.951 kb on - strand, within AMB_RS02005 at 423.046 kb on - strand, within AMB_RS02005 at 423.154 kb on + strand, within AMB_RS02005 at 423.154 kb on + strand, within AMB_RS02005 at 423.154 kb on + strand, within AMB_RS02005 at 423.154 kb on + strand, within AMB_RS02005 at 423.155 kb on - strand, within AMB_RS02005 at 423.308 kb on - strand, within AMB_RS02005 at 423.308 kb on - strand, within AMB_RS02005 at 423.418 kb on + strand at 423.420 kb on + strand at 423.445 kb on + strand at 423.445 kb on + strand at 423.445 kb on + strand at 423.565 kb on + strand at 423.565 kb on + strand at 423.566 kb on - strand at 423.683 kb on + strand, within AMB_RS02010 at 423.684 kb on - strand, within AMB_RS02010 at 423.810 kb on + strand, within AMB_RS02010 at 423.811 kb on - strand, within AMB_RS02010 at 423.891 kb on + strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.892 kb on - strand, within AMB_RS02010 at 423.893 kb on + strand, within AMB_RS02010 at 423.894 kb on - strand, within AMB_RS02010 at 423.894 kb on - strand, within AMB_RS02010 at 424.014 kb on + strand, within AMB_RS02010 at 424.015 kb on - strand, within AMB_RS02010 at 424.287 kb on + strand, within AMB_RS02015 at 424.288 kb on - strand, within AMB_RS02015 at 424.333 kb on + strand, within AMB_RS02015 at 424.333 kb on + strand, within AMB_RS02015 at 424.333 kb on + strand, within AMB_RS02015 at 424.334 kb on - strand, within AMB_RS02015 at 424.334 kb on - strand, within AMB_RS02015 at 424.334 kb on - strand, within AMB_RS02015 at 424.334 kb on - strand, within AMB_RS02015
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 420,572 - AMB_RS01995 0.66 -1.5 420,572 - AMB_RS01995 0.66 -0.8 420,572 - AMB_RS01995 0.66 +0.8 420,712 + +0.4 420,712 + -2.3 420,712 + -1.6 420,713 - -1.4 420,713 - +0.2 420,774 - -0.8 420,788 + -0.1 420,788 + -3.4 420,788 + -0.5 420,788 + -1.2 420,788 + -0.5 420,788 + +0.4 420,788 + -1.1 420,788 + +0.2 420,788 + -1.5 420,788 + -0.6 420,788 + -1.6 420,788 + -0.5 420,788 + -0.1 420,788 + -0.1 420,788 + -0.3 420,788 + +0.3 420,788 + +0.7 420,788 + -0.0 420,788 + +0.6 420,788 + -0.2 420,788 + +0.5 420,789 - -2.5 420,789 - +0.5 420,789 - -0.8 420,789 - -0.1 420,789 - +0.5 420,789 - -0.2 420,789 - -1.6 420,789 - -1.3 420,789 - +0.0 420,789 - -1.1 420,789 - +0.2 420,789 - -1.7 420,789 - +0.5 420,789 - -1.5 420,789 - +0.2 420,789 - -0.2 420,866 + -1.5 420,868 + +0.7 420,868 + +0.1 421,253 + +0.2 421,253 + +0.3 421,253 + -0.5 421,253 + +0.5 421,253 + -0.0 421,253 + +0.1 421,254 - -0.1 421,254 - +0.4 421,254 - +0.0 421,254 - -0.3 421,254 - -0.8 421,254 - +0.5 421,254 - -1.6 421,257 + +0.4 421,257 + -0.5 421,257 + +0.2 421,257 + -1.1 421,258 - -0.5 421,258 - -3.2 421,258 - -1.5 421,258 - -1.1 421,258 - -0.2 421,420 + +0.1 421,420 + -3.3 421,420 + -1.5 421,421 - +0.1 421,421 - -1.6 421,421 - -0.2 421,421 - -2.6 421,421 - +0.4 421,421 - -1.9 421,421 - -1.3 421,556 - AMB_RS02005 0.14 -1.1 421,556 - AMB_RS02005 0.14 -1.5 421,556 - AMB_RS02005 0.14 +0.1 421,588 + AMB_RS02005 0.15 +1.4 421,588 + AMB_RS02005 0.15 -1.3 421,588 + AMB_RS02005 0.15 +0.5 421,589 - AMB_RS02005 0.15 +0.0 421,589 - AMB_RS02005 0.15 +0.7 421,589 - AMB_RS02005 0.15 -2.4 421,589 - AMB_RS02005 0.15 +0.5 421,590 + AMB_RS02005 0.15 -3.0 421,659 + AMB_RS02005 0.18 -0.2 421,660 - AMB_RS02005 0.18 +1.5 421,840 + AMB_RS02005 0.26 +0.4 421,840 + AMB_RS02005 0.26 -0.1 421,849 + AMB_RS02005 0.26 -0.8 421,849 + AMB_RS02005 0.26 -1.1 421,867 + AMB_RS02005 0.27 -3.9 421,867 + AMB_RS02005 0.27 -0.3 421,867 + AMB_RS02005 0.27 -1.3 421,873 + AMB_RS02005 0.27 -0.2 421,873 + AMB_RS02005 0.27 -0.2 422,083 + AMB_RS02005 0.36 -4.3 422,083 + AMB_RS02005 0.36 +0.9 422,084 - AMB_RS02005 0.36 +0.8 422,085 + AMB_RS02005 0.36 +1.1 422,085 + AMB_RS02005 0.36 -0.4 422,085 + AMB_RS02005 0.36 +0.1 422,085 + AMB_RS02005 0.36 -2.2 422,086 - AMB_RS02005 0.36 +0.4 422,086 - AMB_RS02005 0.36 +1.5 422,086 - AMB_RS02005 0.36 +0.5 422,086 - AMB_RS02005 0.36 -0.1 422,086 - AMB_RS02005 0.36 -1.1 422,086 - AMB_RS02005 0.36 -0.4 422,086 - AMB_RS02005 0.36 +0.4 422,086 - AMB_RS02005 0.36 -0.0 422,086 - AMB_RS02005 0.36 +0.0 422,164 + AMB_RS02005 0.40 +0.3 422,164 + AMB_RS02005 0.40 +0.1 422,164 + AMB_RS02005 0.40 -1.1 422,164 + AMB_RS02005 0.40 -0.1 422,250 + AMB_RS02005 0.43 -0.1 422,250 + AMB_RS02005 0.43 -1.1 422,250 + AMB_RS02005 0.43 +0.7 422,250 + AMB_RS02005 0.43 +0.3 422,250 + AMB_RS02005 0.43 -1.6 422,251 - AMB_RS02005 0.43 +0.7 422,251 - AMB_RS02005 0.43 +0.5 422,251 - AMB_RS02005 0.43 +0.2 422,251 - AMB_RS02005 0.43 -0.8 422,251 - AMB_RS02005 0.43 +0.6 422,287 - AMB_RS02005 0.45 -0.5 422,432 - AMB_RS02005 0.51 -0.5 422,432 - AMB_RS02005 0.51 +0.1 422,638 + AMB_RS02005 0.60 +0.0 422,638 + AMB_RS02005 0.60 -2.5 422,733 - AMB_RS02005 0.64 -0.7 422,905 + AMB_RS02005 0.72 -1.8 422,905 + AMB_RS02005 0.72 -2.1 422,905 + AMB_RS02005 0.72 -0.3 422,905 + AMB_RS02005 0.72 -0.5 422,951 - AMB_RS02005 0.73 -0.0 423,046 - AMB_RS02005 0.78 -0.0 423,154 + AMB_RS02005 0.82 +0.5 423,154 + AMB_RS02005 0.82 -1.1 423,154 + AMB_RS02005 0.82 -0.2 423,154 + AMB_RS02005 0.82 -0.8 423,155 - AMB_RS02005 0.82 -0.5 423,308 - AMB_RS02005 0.89 -1.5 423,308 - AMB_RS02005 0.89 -0.9 423,418 + +0.1 423,420 + -0.2 423,445 + -0.3 423,445 + -0.3 423,445 + -1.5 423,565 + +0.3 423,565 + +0.5 423,566 - +0.2 423,683 + AMB_RS02010 0.12 +1.8 423,684 - AMB_RS02010 0.12 -0.1 423,810 + AMB_RS02010 0.41 -1.5 423,811 - AMB_RS02010 0.41 -0.8 423,891 + AMB_RS02010 0.60 +0.0 423,892 - AMB_RS02010 0.60 -1.1 423,892 - AMB_RS02010 0.60 +0.3 423,892 - AMB_RS02010 0.60 +1.0 423,892 - AMB_RS02010 0.60 +0.1 423,892 - AMB_RS02010 0.60 -1.3 423,892 - AMB_RS02010 0.60 +0.3 423,892 - AMB_RS02010 0.60 -0.1 423,893 + AMB_RS02010 0.60 -1.3 423,894 - AMB_RS02010 0.60 -2.6 423,894 - AMB_RS02010 0.60 -0.1 424,014 + AMB_RS02010 0.88 -0.2 424,015 - AMB_RS02010 0.88 -1.8 424,287 + AMB_RS02015 0.36 +0.3 424,288 - AMB_RS02015 0.37 -0.1 424,333 + AMB_RS02015 0.44 -1.3 424,333 + AMB_RS02015 0.44 +0.5 424,333 + AMB_RS02015 0.44 -0.1 424,334 - AMB_RS02015 0.44 +0.5 424,334 - AMB_RS02015 0.44 -0.4 424,334 - AMB_RS02015 0.44 +0.3 424,334 - AMB_RS02015 0.44 +0.5
Or see this region's nucleotide sequence