Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01435 and AMB_RS01440 are separated by 17 nucleotides AMB_RS01440 and AMB_RS01445 are separated by 169 nucleotides AMB_RS01445 and AMB_RS01450 overlap by 4 nucleotides AMB_RS01450 and AMB_RS01455 overlap by 4 nucleotides
AMB_RS01435: AMB_RS01435 - M23 family peptidase, at 312,950 to 313,864
_RS01435
AMB_RS01440: AMB_RS01440 - hypothetical protein, at 313,882 to 314,670
_RS01440
AMB_RS01445: AMB_RS01445 - hypothetical protein, at 314,840 to 318,310
_RS01445
AMB_RS01450: AMB_RS01450 - hypothetical protein, at 318,307 to 319,227
_RS01450
AMB_RS01455: AMB_RS01455 - DNA mismatch repair protein MutS, at 319,224 to 321,830
_RS01455
Position (kb) 314
315
316
317
318
319 Strain fitness (log2 ratio) -2
-1
0
1 at 313.841 kb on + strand at 313.841 kb on + strand at 313.842 kb on - strand at 313.842 kb on - strand at 313.842 kb on - strand at 313.842 kb on - strand at 313.864 kb on + strand at 313.865 kb on - strand at 313.865 kb on - strand at 313.865 kb on - strand at 313.865 kb on - strand at 313.865 kb on - strand at 313.883 kb on + strand at 313.883 kb on + strand at 313.884 kb on - strand at 313.884 kb on - strand at 313.884 kb on - strand at 313.884 kb on - strand at 313.884 kb on - strand at 313.884 kb on - strand at 313.884 kb on - strand at 314.214 kb on + strand, within AMB_RS01440 at 314.214 kb on + strand, within AMB_RS01440 at 314.214 kb on + strand, within AMB_RS01440 at 314.214 kb on + strand, within AMB_RS01440 at 314.214 kb on + strand, within AMB_RS01440 at 314.214 kb on + strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.215 kb on - strand, within AMB_RS01440 at 314.216 kb on + strand, within AMB_RS01440 at 314.217 kb on - strand, within AMB_RS01440 at 314.217 kb on - strand, within AMB_RS01440 at 314.705 kb on + strand at 314.705 kb on + strand at 314.705 kb on + strand at 314.706 kb on - strand at 314.706 kb on - strand at 314.706 kb on - strand at 314.706 kb on - strand at 314.706 kb on - strand at 314.710 kb on + strand at 314.711 kb on - strand at 314.736 kb on + strand at 314.736 kb on + strand at 314.736 kb on + strand at 318.542 kb on + strand, within AMB_RS01450 at 318.542 kb on + strand, within AMB_RS01450 at 318.543 kb on - strand, within AMB_RS01450 at 318.543 kb on - strand, within AMB_RS01450 at 318.543 kb on - strand, within AMB_RS01450 at 318.810 kb on - strand, within AMB_RS01450 at 319.246 kb on + strand at 319.246 kb on + strand at 319.246 kb on + strand at 319.246 kb on + strand at 319.247 kb on - strand at 319.247 kb on - strand at 319.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 313,841 + -0.5 313,841 + +0.1 313,842 - -0.1 313,842 - -0.8 313,842 - -0.2 313,842 - -1.0 313,864 + +0.5 313,865 - +0.1 313,865 - -0.6 313,865 - -0.0 313,865 - -0.8 313,865 - +0.2 313,883 + +0.2 313,883 + +0.4 313,884 - -1.6 313,884 - -0.8 313,884 - -2.5 313,884 - +0.0 313,884 - +0.2 313,884 - -0.0 313,884 - -1.6 314,214 + AMB_RS01440 0.42 +0.2 314,214 + AMB_RS01440 0.42 -1.3 314,214 + AMB_RS01440 0.42 -0.0 314,214 + AMB_RS01440 0.42 -0.2 314,214 + AMB_RS01440 0.42 +0.0 314,214 + AMB_RS01440 0.42 -1.8 314,215 - AMB_RS01440 0.42 +0.3 314,215 - AMB_RS01440 0.42 -0.0 314,215 - AMB_RS01440 0.42 +0.3 314,215 - AMB_RS01440 0.42 -0.4 314,215 - AMB_RS01440 0.42 +1.0 314,215 - AMB_RS01440 0.42 -0.2 314,215 - AMB_RS01440 0.42 -1.0 314,215 - AMB_RS01440 0.42 -1.0 314,216 + AMB_RS01440 0.42 -0.5 314,217 - AMB_RS01440 0.42 -0.5 314,217 - AMB_RS01440 0.42 +0.4 314,705 + +0.5 314,705 + +0.2 314,705 + -1.2 314,706 - -0.7 314,706 - -1.6 314,706 - -1.2 314,706 - -1.0 314,706 - -0.1 314,710 + -1.8 314,711 - -0.5 314,736 + -0.8 314,736 + -0.0 314,736 + -0.2 318,542 + AMB_RS01450 0.26 -0.1 318,542 + AMB_RS01450 0.26 +0.5 318,543 - AMB_RS01450 0.26 +0.1 318,543 - AMB_RS01450 0.26 +0.7 318,543 - AMB_RS01450 0.26 -2.3 318,810 - AMB_RS01450 0.55 -1.6 319,246 + -0.4 319,246 + +0.4 319,246 + -1.5 319,246 + -2.0 319,247 - -0.5 319,247 - -0.8 319,247 - -0.5
Or see this region's nucleotide sequence