Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01355 and AMB_RS01360 are separated by 10 nucleotides AMB_RS01360 and AMB_RS01365 are separated by 102 nucleotides
AMB_RS01355: AMB_RS01355 - sphingosine kinase, at 299,119 to 300,000
_RS01355
AMB_RS01360: AMB_RS01360 - hypothetical protein, at 300,011 to 300,376
_RS01360
AMB_RS01365: AMB_RS01365 - nitroreductase, at 300,479 to 302,077
_RS01365
Position (kb) 300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 299.035 kb on - strand at 299.057 kb on - strand at 299.605 kb on + strand, within AMB_RS01355 at 299.606 kb on - strand, within AMB_RS01355 at 299.606 kb on - strand, within AMB_RS01355 at 299.606 kb on - strand, within AMB_RS01355 at 299.681 kb on - strand, within AMB_RS01355 at 299.719 kb on + strand, within AMB_RS01355 at 299.719 kb on + strand, within AMB_RS01355 at 299.719 kb on + strand, within AMB_RS01355 at 299.960 kb on + strand at 299.960 kb on + strand at 299.960 kb on + strand at 299.960 kb on + strand at 299.961 kb on - strand at 299.961 kb on - strand at 299.961 kb on - strand at 300.012 kb on + strand at 300.013 kb on - strand at 300.013 kb on - strand at 300.282 kb on + strand, within AMB_RS01360 at 300.282 kb on + strand, within AMB_RS01360 at 300.282 kb on + strand, within AMB_RS01360 at 300.282 kb on + strand, within AMB_RS01360 at 300.283 kb on - strand, within AMB_RS01360 at 300.283 kb on - strand, within AMB_RS01360 at 300.283 kb on - strand, within AMB_RS01360 at 300.405 kb on + strand at 300.405 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.426 kb on + strand at 300.427 kb on - strand at 300.427 kb on - strand at 300.427 kb on - strand at 300.428 kb on + strand at 300.428 kb on + strand at 300.428 kb on + strand at 300.428 kb on + strand at 300.428 kb on + strand at 300.428 kb on + strand at 300.428 kb on + strand at 300.429 kb on - strand at 300.429 kb on - strand at 300.429 kb on - strand at 300.429 kb on - strand at 300.429 kb on - strand at 300.429 kb on - strand at 300.478 kb on + strand at 300.478 kb on + strand at 300.478 kb on + strand at 300.479 kb on - strand at 300.542 kb on + strand at 300.866 kb on + strand, within AMB_RS01365 at 300.867 kb on - strand, within AMB_RS01365 at 300.867 kb on - strand, within AMB_RS01365 at 300.867 kb on - strand, within AMB_RS01365 at 300.872 kb on + strand, within AMB_RS01365 at 300.872 kb on + strand, within AMB_RS01365 at 300.873 kb on - strand, within AMB_RS01365 at 300.992 kb on + strand, within AMB_RS01365 at 300.992 kb on + strand, within AMB_RS01365 at 300.992 kb on + strand, within AMB_RS01365 at 300.992 kb on + strand, within AMB_RS01365 at 300.993 kb on - strand, within AMB_RS01365 at 301.014 kb on - strand, within AMB_RS01365 at 301.056 kb on - strand, within AMB_RS01365 at 301.056 kb on - strand, within AMB_RS01365 at 301.267 kb on + strand, within AMB_RS01365
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 299,035 - +0.5 299,057 - -0.4 299,605 + AMB_RS01355 0.55 +0.4 299,606 - AMB_RS01355 0.55 +0.8 299,606 - AMB_RS01355 0.55 +0.3 299,606 - AMB_RS01355 0.55 +0.3 299,681 - AMB_RS01355 0.64 +0.1 299,719 + AMB_RS01355 0.68 +0.5 299,719 + AMB_RS01355 0.68 -1.4 299,719 + AMB_RS01355 0.68 -1.0 299,960 + -2.1 299,960 + +0.3 299,960 + +0.6 299,960 + -1.3 299,961 - -0.0 299,961 - -0.0 299,961 - -1.3 300,012 + -0.1 300,013 - -0.1 300,013 - +0.5 300,282 + AMB_RS01360 0.74 +0.6 300,282 + AMB_RS01360 0.74 +0.4 300,282 + AMB_RS01360 0.74 -0.0 300,282 + AMB_RS01360 0.74 +0.7 300,283 - AMB_RS01360 0.74 +0.6 300,283 - AMB_RS01360 0.74 +0.2 300,283 - AMB_RS01360 0.74 +0.3 300,405 + -0.4 300,405 + +0.9 300,426 + +0.3 300,426 + +0.0 300,426 + -1.6 300,426 + +0.3 300,426 + -0.9 300,426 + -1.0 300,426 + -2.4 300,426 + +0.1 300,426 + +1.6 300,426 + -2.4 300,426 + +1.6 300,426 + -0.4 300,426 + +1.1 300,427 - -1.9 300,427 - +0.1 300,427 - +0.2 300,428 + +0.6 300,428 + -0.0 300,428 + +0.2 300,428 + +0.5 300,428 + -0.4 300,428 + -2.0 300,428 + +0.1 300,429 - -0.9 300,429 - +0.3 300,429 - -2.4 300,429 - -3.6 300,429 - -0.6 300,429 - -0.6 300,478 + -1.9 300,478 + +1.0 300,478 + -0.8 300,479 - -2.1 300,542 + +0.4 300,866 + AMB_RS01365 0.24 -0.6 300,867 - AMB_RS01365 0.24 -0.8 300,867 - AMB_RS01365 0.24 -0.8 300,867 - AMB_RS01365 0.24 +0.7 300,872 + AMB_RS01365 0.25 +1.6 300,872 + AMB_RS01365 0.25 +0.2 300,873 - AMB_RS01365 0.25 +0.6 300,992 + AMB_RS01365 0.32 +1.6 300,992 + AMB_RS01365 0.32 +0.3 300,992 + AMB_RS01365 0.32 +0.3 300,992 + AMB_RS01365 0.32 -2.1 300,993 - AMB_RS01365 0.32 -0.0 301,014 - AMB_RS01365 0.33 +0.2 301,056 - AMB_RS01365 0.36 -0.0 301,056 - AMB_RS01365 0.36 +1.8 301,267 + AMB_RS01365 0.49 -0.0
Or see this region's nucleotide sequence