Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01320 and AMB_RS01325 are separated by 36 nucleotides AMB_RS01325 and AMB_RS01330 overlap by 4 nucleotides
AMB_RS01320: AMB_RS01320 - MFS transporter, at 290,708 to 292,105
_RS01320
AMB_RS01325: AMB_RS01325 - phosphatase PAP2 family protein, at 292,142 to 292,930
_RS01325
AMB_RS01330: AMB_RS01330 - glycosyltransferase family 39 protein, at 292,927 to 294,570
_RS01330
Position (kb) 292
293 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 291.320 kb on + strand, within AMB_RS01320 at 291.321 kb on - strand, within AMB_RS01320 at 291.321 kb on - strand, within AMB_RS01320 at 291.479 kb on + strand, within AMB_RS01320 at 291.479 kb on + strand, within AMB_RS01320 at 291.479 kb on + strand, within AMB_RS01320 at 291.479 kb on + strand, within AMB_RS01320 at 291.479 kb on + strand, within AMB_RS01320 at 291.480 kb on - strand, within AMB_RS01320 at 291.480 kb on - strand, within AMB_RS01320 at 291.480 kb on - strand, within AMB_RS01320 at 291.480 kb on - strand, within AMB_RS01320 at 291.480 kb on - strand, within AMB_RS01320 at 291.480 kb on - strand, within AMB_RS01320 at 291.512 kb on + strand, within AMB_RS01320 at 291.512 kb on + strand, within AMB_RS01320 at 291.513 kb on - strand, within AMB_RS01320 at 291.513 kb on - strand, within AMB_RS01320 at 291.513 kb on - strand, within AMB_RS01320 at 291.537 kb on - strand, within AMB_RS01320 at 291.537 kb on - strand, within AMB_RS01320 at 291.537 kb on - strand, within AMB_RS01320 at 291.698 kb on + strand, within AMB_RS01320 at 291.698 kb on + strand, within AMB_RS01320 at 291.698 kb on + strand, within AMB_RS01320 at 291.699 kb on - strand, within AMB_RS01320 at 291.699 kb on - strand, within AMB_RS01320 at 291.699 kb on - strand, within AMB_RS01320 at 291.836 kb on + strand, within AMB_RS01320 at 291.836 kb on + strand, within AMB_RS01320 at 291.837 kb on - strand, within AMB_RS01320 at 292.144 kb on - strand at 292.335 kb on + strand, within AMB_RS01325 at 292.416 kb on + strand, within AMB_RS01325 at 292.416 kb on + strand, within AMB_RS01325 at 292.417 kb on - strand, within AMB_RS01325 at 292.444 kb on - strand, within AMB_RS01325 at 292.444 kb on - strand, within AMB_RS01325 at 292.632 kb on - strand, within AMB_RS01325 at 292.632 kb on - strand, within AMB_RS01325 at 292.782 kb on + strand, within AMB_RS01325 at 292.783 kb on - strand, within AMB_RS01325 at 292.952 kb on + strand at 292.985 kb on + strand at 292.985 kb on + strand at 293.255 kb on + strand, within AMB_RS01330 at 293.255 kb on + strand, within AMB_RS01330 at 293.591 kb on + strand, within AMB_RS01330 at 293.591 kb on + strand, within AMB_RS01330 at 293.591 kb on + strand, within AMB_RS01330 at 293.591 kb on + strand, within AMB_RS01330 at 293.592 kb on - strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330 at 293.756 kb on + strand, within AMB_RS01330
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 291,320 + AMB_RS01320 0.44 -2.8 291,321 - AMB_RS01320 0.44 -1.8 291,321 - AMB_RS01320 0.44 -0.4 291,479 + AMB_RS01320 0.55 -0.4 291,479 + AMB_RS01320 0.55 +0.2 291,479 + AMB_RS01320 0.55 +0.6 291,479 + AMB_RS01320 0.55 -1.0 291,479 + AMB_RS01320 0.55 +0.1 291,480 - AMB_RS01320 0.55 -0.1 291,480 - AMB_RS01320 0.55 -1.0 291,480 - AMB_RS01320 0.55 -1.9 291,480 - AMB_RS01320 0.55 -2.0 291,480 - AMB_RS01320 0.55 -1.6 291,480 - AMB_RS01320 0.55 -1.0 291,512 + AMB_RS01320 0.58 -0.5 291,512 + AMB_RS01320 0.58 -1.4 291,513 - AMB_RS01320 0.58 +0.6 291,513 - AMB_RS01320 0.58 -0.0 291,513 - AMB_RS01320 0.58 +0.1 291,537 - AMB_RS01320 0.59 -0.4 291,537 - AMB_RS01320 0.59 +0.1 291,537 - AMB_RS01320 0.59 +0.2 291,698 + AMB_RS01320 0.71 +0.6 291,698 + AMB_RS01320 0.71 -0.6 291,698 + AMB_RS01320 0.71 -1.4 291,699 - AMB_RS01320 0.71 -0.6 291,699 - AMB_RS01320 0.71 +0.4 291,699 - AMB_RS01320 0.71 +0.1 291,836 + AMB_RS01320 0.81 +0.1 291,836 + AMB_RS01320 0.81 -1.2 291,837 - AMB_RS01320 0.81 -3.2 292,144 - -0.0 292,335 + AMB_RS01325 0.24 -2.2 292,416 + AMB_RS01325 0.35 -1.8 292,416 + AMB_RS01325 0.35 +1.6 292,417 - AMB_RS01325 0.35 -2.6 292,444 - AMB_RS01325 0.38 -0.0 292,444 - AMB_RS01325 0.38 -0.4 292,632 - AMB_RS01325 0.62 -2.1 292,632 - AMB_RS01325 0.62 -2.8 292,782 + AMB_RS01325 0.81 -3.0 292,783 - AMB_RS01325 0.81 +0.4 292,952 + -0.4 292,985 + +0.6 292,985 + -3.7 293,255 + AMB_RS01330 0.20 -1.0 293,255 + AMB_RS01330 0.20 -5.2 293,591 + AMB_RS01330 0.40 -1.2 293,591 + AMB_RS01330 0.40 -1.4 293,591 + AMB_RS01330 0.40 -4.0 293,591 + AMB_RS01330 0.40 -4.1 293,592 - AMB_RS01330 0.40 -1.2 293,756 + AMB_RS01330 0.50 -1.8 293,756 + AMB_RS01330 0.50 +1.6 293,756 + AMB_RS01330 0.50 -4.9 293,756 + AMB_RS01330 0.50 +0.6 293,756 + AMB_RS01330 0.50 -2.7 293,756 + AMB_RS01330 0.50 -0.4 293,756 + AMB_RS01330 0.50 -2.4 293,756 + AMB_RS01330 0.50 -0.4
Or see this region's nucleotide sequence