Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01265 and AMB_RS01270 are separated by 110 nucleotides AMB_RS01270 and AMB_RS01275 overlap by 4 nucleotides
AMB_RS01265: AMB_RS01265 - LysR family transcriptional regulator, at 280,573 to 281,460
_RS01265
AMB_RS01270: AMB_RS01270 - 4-oxalomesaconate tautomerase, at 281,571 to 282,662
_RS01270
AMB_RS01275: AMB_RS01275 - amidohydrolase, at 282,659 to 283,687
_RS01275
Position (kb) 281
282
283 Strain fitness (log2 ratio) -2
-1
0
1 at 280.595 kb on - strand at 281.013 kb on + strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.475 kb on + strand at 281.948 kb on + strand, within AMB_RS01270 at 281.949 kb on - strand, within AMB_RS01270 at 281.955 kb on - strand, within AMB_RS01270 at 282.000 kb on + strand, within AMB_RS01270 at 282.000 kb on + strand, within AMB_RS01270 at 282.000 kb on + strand, within AMB_RS01270 at 282.001 kb on - strand, within AMB_RS01270 at 282.001 kb on - strand, within AMB_RS01270 at 282.001 kb on - strand, within AMB_RS01270 at 282.086 kb on + strand, within AMB_RS01270 at 282.087 kb on - strand, within AMB_RS01270 at 282.087 kb on - strand, within AMB_RS01270 at 282.169 kb on + strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.683 kb on + strand at 282.683 kb on + strand at 282.683 kb on + strand at 282.684 kb on - strand at 282.684 kb on - strand at 282.684 kb on - strand at 283.061 kb on + strand, within AMB_RS01275 at 283.061 kb on + strand, within AMB_RS01275 at 283.061 kb on + strand, within AMB_RS01275 at 283.061 kb on + strand, within AMB_RS01275 at 283.061 kb on + strand, within AMB_RS01275 at 283.062 kb on - strand, within AMB_RS01275 at 283.062 kb on - strand, within AMB_RS01275 at 283.062 kb on - strand, within AMB_RS01275 at 283.151 kb on + strand, within AMB_RS01275 at 283.151 kb on + strand, within AMB_RS01275 at 283.152 kb on - strand, within AMB_RS01275 at 283.152 kb on - strand, within AMB_RS01275 at 283.152 kb on - strand, within AMB_RS01275 at 283.169 kb on + strand, within AMB_RS01275 at 283.169 kb on + strand, within AMB_RS01275 at 283.238 kb on + strand, within AMB_RS01275 at 283.238 kb on + strand, within AMB_RS01275 at 283.239 kb on - strand, within AMB_RS01275 at 283.239 kb on - strand, within AMB_RS01275 at 283.346 kb on + strand, within AMB_RS01275 at 283.346 kb on + strand, within AMB_RS01275 at 283.432 kb on + strand, within AMB_RS01275 at 283.442 kb on + strand, within AMB_RS01275 at 283.553 kb on + strand, within AMB_RS01275 at 283.553 kb on + strand, within AMB_RS01275 at 283.554 kb on - strand, within AMB_RS01275 at 283.556 kb on + strand, within AMB_RS01275 at 283.556 kb on + strand, within AMB_RS01275 at 283.556 kb on + strand, within AMB_RS01275 at 283.556 kb on + strand, within AMB_RS01275 at 283.574 kb on + strand, within AMB_RS01275 at 283.574 kb on + strand, within AMB_RS01275 at 283.575 kb on - strand, within AMB_RS01275 at 283.575 kb on - strand, within AMB_RS01275 at 283.575 kb on - strand, within AMB_RS01275 at 283.575 kb on - strand, within AMB_RS01275 at 283.622 kb on + strand at 283.622 kb on + strand at 283.622 kb on + strand at 283.646 kb on + strand at 283.646 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 280,595 - -1.0 281,013 + AMB_RS01265 0.50 -0.7 281,014 - AMB_RS01265 0.50 -2.0 281,014 - AMB_RS01265 0.50 -0.4 281,014 - AMB_RS01265 0.50 -1.6 281,475 + -0.4 281,948 + AMB_RS01270 0.35 +0.3 281,949 - AMB_RS01270 0.35 -1.0 281,955 - AMB_RS01270 0.35 -0.5 282,000 + AMB_RS01270 0.39 -2.6 282,000 + AMB_RS01270 0.39 +0.4 282,000 + AMB_RS01270 0.39 +0.6 282,001 - AMB_RS01270 0.39 +0.1 282,001 - AMB_RS01270 0.39 -1.0 282,001 - AMB_RS01270 0.39 -0.4 282,086 + AMB_RS01270 0.47 -0.4 282,087 - AMB_RS01270 0.47 -1.6 282,087 - AMB_RS01270 0.47 -0.4 282,169 + AMB_RS01270 0.55 -1.4 282,170 - AMB_RS01270 0.55 -1.5 282,170 - AMB_RS01270 0.55 +0.0 282,170 - AMB_RS01270 0.55 -0.4 282,170 - AMB_RS01270 0.55 -0.7 282,315 + AMB_RS01270 0.68 -0.4 282,315 + AMB_RS01270 0.68 +0.1 282,315 + AMB_RS01270 0.68 +0.0 282,315 + AMB_RS01270 0.68 -0.0 282,683 + -0.8 282,683 + +0.4 282,683 + +0.1 282,684 - -1.0 282,684 - -0.2 282,684 - -0.7 283,061 + AMB_RS01275 0.39 +0.5 283,061 + AMB_RS01275 0.39 -1.0 283,061 + AMB_RS01275 0.39 -1.4 283,061 + AMB_RS01275 0.39 -1.2 283,061 + AMB_RS01275 0.39 -2.1 283,062 - AMB_RS01275 0.39 +1.6 283,062 - AMB_RS01275 0.39 +0.0 283,062 - AMB_RS01275 0.39 -1.6 283,151 + AMB_RS01275 0.48 -0.3 283,151 + AMB_RS01275 0.48 +0.0 283,152 - AMB_RS01275 0.48 -0.2 283,152 - AMB_RS01275 0.48 -0.3 283,152 - AMB_RS01275 0.48 -1.9 283,169 + AMB_RS01275 0.50 +1.6 283,169 + AMB_RS01275 0.50 -0.7 283,238 + AMB_RS01275 0.56 +0.5 283,238 + AMB_RS01275 0.56 -0.3 283,239 - AMB_RS01275 0.56 +0.1 283,239 - AMB_RS01275 0.56 -1.2 283,346 + AMB_RS01275 0.67 -1.2 283,346 + AMB_RS01275 0.67 +0.8 283,432 + AMB_RS01275 0.75 -0.7 283,442 + AMB_RS01275 0.76 -0.7 283,553 + AMB_RS01275 0.87 -0.1 283,553 + AMB_RS01275 0.87 +0.6 283,554 - AMB_RS01275 0.87 -0.0 283,556 + AMB_RS01275 0.87 -1.6 283,556 + AMB_RS01275 0.87 +0.3 283,556 + AMB_RS01275 0.87 -2.1 283,556 + AMB_RS01275 0.87 +0.3 283,574 + AMB_RS01275 0.89 +0.6 283,574 + AMB_RS01275 0.89 +0.5 283,575 - AMB_RS01275 0.89 -1.0 283,575 - AMB_RS01275 0.89 -1.2 283,575 - AMB_RS01275 0.89 +0.4 283,575 - AMB_RS01275 0.89 +0.1 283,622 + +0.3 283,622 + -2.4 283,622 + +0.6 283,646 + +0.2 283,646 + +0.6
Or see this region's nucleotide sequence