Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01255 and AMB_RS01260 overlap by 1 nucleotides AMB_RS01260 and AMB_RS01265 are separated by 111 nucleotides AMB_RS01265 and AMB_RS01270 are separated by 110 nucleotides
AMB_RS01255: AMB_RS01255 - 7-carboxy-7-deazaguanine synthase, at 279,329 to 279,937
_RS01255
AMB_RS01260: AMB_RS01260 - 6-carboxytetrahydropterin synthase, at 279,937 to 280,461
_RS01260
AMB_RS01265: AMB_RS01265 - LysR family transcriptional regulator, at 280,573 to 281,460
_RS01265
AMB_RS01270: AMB_RS01270 - 4-oxalomesaconate tautomerase, at 281,571 to 282,662
_RS01270
Position (kb) 280
281
282 Strain fitness (log2 ratio) -2
-1
0
1
2 at 279.809 kb on + strand, within AMB_RS01255 at 279.810 kb on - strand, within AMB_RS01255 at 279.810 kb on - strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.853 kb on + strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.854 kb on - strand, within AMB_RS01255 at 279.935 kb on + strand at 279.935 kb on + strand at 279.935 kb on + strand at 280.033 kb on + strand, within AMB_RS01260 at 280.033 kb on + strand, within AMB_RS01260 at 280.033 kb on + strand, within AMB_RS01260 at 280.033 kb on + strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.034 kb on - strand, within AMB_RS01260 at 280.303 kb on + strand, within AMB_RS01260 at 280.303 kb on + strand, within AMB_RS01260 at 280.303 kb on + strand, within AMB_RS01260 at 280.304 kb on - strand, within AMB_RS01260 at 280.304 kb on - strand, within AMB_RS01260 at 280.447 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.468 kb on + strand at 280.469 kb on - strand at 280.469 kb on - strand at 280.477 kb on - strand at 280.477 kb on - strand at 280.498 kb on + strand at 280.498 kb on + strand at 280.539 kb on + strand at 280.595 kb on - strand at 281.013 kb on + strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.014 kb on - strand, within AMB_RS01265 at 281.475 kb on + strand at 281.948 kb on + strand, within AMB_RS01270 at 281.949 kb on - strand, within AMB_RS01270 at 281.955 kb on - strand, within AMB_RS01270 at 282.000 kb on + strand, within AMB_RS01270 at 282.000 kb on + strand, within AMB_RS01270 at 282.000 kb on + strand, within AMB_RS01270 at 282.001 kb on - strand, within AMB_RS01270 at 282.001 kb on - strand, within AMB_RS01270 at 282.001 kb on - strand, within AMB_RS01270 at 282.086 kb on + strand, within AMB_RS01270 at 282.087 kb on - strand, within AMB_RS01270 at 282.087 kb on - strand, within AMB_RS01270 at 282.169 kb on + strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.170 kb on - strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270 at 282.315 kb on + strand, within AMB_RS01270
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 279,809 + AMB_RS01255 0.79 +0.6 279,810 - AMB_RS01255 0.79 -1.2 279,810 - AMB_RS01255 0.79 +0.5 279,853 + AMB_RS01255 0.86 +0.3 279,853 + AMB_RS01255 0.86 -2.4 279,853 + AMB_RS01255 0.86 +0.2 279,853 + AMB_RS01255 0.86 -0.1 279,853 + AMB_RS01255 0.86 +1.6 279,854 - AMB_RS01255 0.86 -0.2 279,854 - AMB_RS01255 0.86 -0.7 279,854 - AMB_RS01255 0.86 -0.2 279,854 - AMB_RS01255 0.86 -1.0 279,854 - AMB_RS01255 0.86 -2.7 279,854 - AMB_RS01255 0.86 +2.2 279,935 + +0.6 279,935 + +0.4 279,935 + -1.9 280,033 + AMB_RS01260 0.18 -1.0 280,033 + AMB_RS01260 0.18 -0.2 280,033 + AMB_RS01260 0.18 -0.4 280,033 + AMB_RS01260 0.18 -0.7 280,034 - AMB_RS01260 0.18 -0.6 280,034 - AMB_RS01260 0.18 +0.2 280,034 - AMB_RS01260 0.18 -1.4 280,034 - AMB_RS01260 0.18 -2.1 280,303 + AMB_RS01260 0.70 +0.3 280,303 + AMB_RS01260 0.70 -1.2 280,303 + AMB_RS01260 0.70 -1.4 280,304 - AMB_RS01260 0.70 -0.1 280,304 - AMB_RS01260 0.70 +0.4 280,447 + -0.2 280,468 + -0.1 280,468 + +0.2 280,468 + -0.4 280,468 + +0.3 280,468 + +0.2 280,468 + -1.6 280,468 + +0.1 280,469 - -0.4 280,469 - +0.1 280,477 - +0.6 280,477 - -0.4 280,498 + -2.1 280,498 + -0.1 280,539 + +0.2 280,595 - -1.0 281,013 + AMB_RS01265 0.50 -0.7 281,014 - AMB_RS01265 0.50 -2.0 281,014 - AMB_RS01265 0.50 -0.4 281,014 - AMB_RS01265 0.50 -1.6 281,475 + -0.4 281,948 + AMB_RS01270 0.35 +0.3 281,949 - AMB_RS01270 0.35 -1.0 281,955 - AMB_RS01270 0.35 -0.5 282,000 + AMB_RS01270 0.39 -2.6 282,000 + AMB_RS01270 0.39 +0.4 282,000 + AMB_RS01270 0.39 +0.6 282,001 - AMB_RS01270 0.39 +0.1 282,001 - AMB_RS01270 0.39 -1.0 282,001 - AMB_RS01270 0.39 -0.4 282,086 + AMB_RS01270 0.47 -0.4 282,087 - AMB_RS01270 0.47 -1.6 282,087 - AMB_RS01270 0.47 -0.4 282,169 + AMB_RS01270 0.55 -1.4 282,170 - AMB_RS01270 0.55 -1.5 282,170 - AMB_RS01270 0.55 +0.0 282,170 - AMB_RS01270 0.55 -0.4 282,170 - AMB_RS01270 0.55 -0.7 282,315 + AMB_RS01270 0.68 -0.4 282,315 + AMB_RS01270 0.68 +0.1 282,315 + AMB_RS01270 0.68 +0.0 282,315 + AMB_RS01270 0.68 -0.0
Or see this region's nucleotide sequence