Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00885 and AMB_RS00890 are separated by 5 nucleotides AMB_RS00890 and AMB_RS00895 are separated by 52 nucleotides
AMB_RS00885: AMB_RS00885 - 2,3-epoxybenzoyl-CoA dihydrolase, at 199,634 to 201,286
_RS00885
AMB_RS00890: AMB_RS00890 - benzoyl-CoA 2,3-epoxidase subunit BoxB, at 201,292 to 202,713
_RS00890
AMB_RS00895: AMB_RS00895 - benzoyl-CoA 2,3-epoxidase subunit BoxA, at 202,766 to 203,947
_RS00895
Position (kb)
201
202
203 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 200.399 kb on + strand, within AMB_RS00885 at 200.399 kb on + strand, within AMB_RS00885 at 200.399 kb on + strand, within AMB_RS00885 at 200.399 kb on + strand, within AMB_RS00885 at 200.399 kb on + strand, within AMB_RS00885 at 200.400 kb on - strand, within AMB_RS00885 at 200.446 kb on + strand, within AMB_RS00885 at 200.446 kb on + strand, within AMB_RS00885 at 200.446 kb on + strand, within AMB_RS00885 at 200.519 kb on + strand, within AMB_RS00885 at 200.636 kb on + strand, within AMB_RS00885 at 200.697 kb on - strand, within AMB_RS00885 at 200.697 kb on - strand, within AMB_RS00885 at 200.697 kb on - strand, within AMB_RS00885 at 201.242 kb on + strand at 201.267 kb on - strand at 201.284 kb on + strand at 201.284 kb on + strand at 201.307 kb on + strand at 201.307 kb on + strand at 201.308 kb on - strand at 201.388 kb on + strand at 201.388 kb on + strand at 201.388 kb on + strand at 201.388 kb on + strand at 201.389 kb on - strand at 201.389 kb on - strand at 201.390 kb on + strand at 201.390 kb on + strand at 201.390 kb on + strand at 201.391 kb on - strand at 201.391 kb on - strand at 201.442 kb on + strand, within AMB_RS00890 at 201.442 kb on + strand, within AMB_RS00890 at 201.442 kb on + strand, within AMB_RS00890 at 201.442 kb on + strand, within AMB_RS00890 at 201.443 kb on - strand, within AMB_RS00890 at 201.443 kb on - strand, within AMB_RS00890 at 201.443 kb on - strand, within AMB_RS00890 at 201.443 kb on - strand, within AMB_RS00890 at 201.511 kb on + strand, within AMB_RS00890 at 201.610 kb on + strand, within AMB_RS00890 at 201.710 kb on - strand, within AMB_RS00890 at 201.710 kb on - strand, within AMB_RS00890 at 201.772 kb on + strand, within AMB_RS00890 at 201.773 kb on - strand, within AMB_RS00890 at 201.773 kb on - strand, within AMB_RS00890 at 201.773 kb on - strand, within AMB_RS00890 at 201.943 kb on + strand, within AMB_RS00890 at 201.944 kb on - strand, within AMB_RS00890 at 202.183 kb on + strand, within AMB_RS00890 at 202.219 kb on + strand, within AMB_RS00890 at 202.219 kb on + strand, within AMB_RS00890 at 202.220 kb on - strand, within AMB_RS00890 at 202.220 kb on - strand, within AMB_RS00890 at 202.291 kb on + strand, within AMB_RS00890 at 202.291 kb on + strand, within AMB_RS00890 at 202.291 kb on + strand, within AMB_RS00890 at 202.292 kb on - strand, within AMB_RS00890 at 202.292 kb on - strand, within AMB_RS00890 at 202.591 kb on + strand at 202.591 kb on + strand at 202.592 kb on - strand at 202.592 kb on - strand at 202.592 kb on - strand at 202.674 kb on + strand at 202.697 kb on - strand at 202.697 kb on - strand at 202.712 kb on - strand at 202.712 kb on - strand at 202.712 kb on - strand at 202.871 kb on + strand at 202.871 kb on + strand at 202.871 kb on + strand at 202.871 kb on + strand at 202.872 kb on - strand at 202.872 kb on - strand at 202.872 kb on - strand at 202.872 kb on - strand at 202.872 kb on - strand at 202.883 kb on + strand at 202.883 kb on + strand at 203.135 kb on + strand, within AMB_RS00895 at 203.136 kb on - strand, within AMB_RS00895 at 203.147 kb on + strand, within AMB_RS00895 at 203.147 kb on + strand, within AMB_RS00895 at 203.189 kb on - strand, within AMB_RS00895 at 203.189 kb on - strand, within AMB_RS00895 at 203.189 kb on - strand, within AMB_RS00895 at 203.339 kb on + strand, within AMB_RS00895 at 203.340 kb on - strand, within AMB_RS00895 at 203.340 kb on - strand, within AMB_RS00895 at 203.340 kb on - strand, within AMB_RS00895 at 203.432 kb on + strand, within AMB_RS00895 at 203.432 kb on + strand, within AMB_RS00895 at 203.433 kb on - strand, within AMB_RS00895 at 203.433 kb on - strand, within AMB_RS00895 at 203.433 kb on - strand, within AMB_RS00895 at 203.654 kb on + strand, within AMB_RS00895
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 200,399 + AMB_RS00885 0.46 +0.5 200,399 + AMB_RS00885 0.46 +1.6 200,399 + AMB_RS00885 0.46 +0.9 200,399 + AMB_RS00885 0.46 +0.7 200,399 + AMB_RS00885 0.46 +1.6 200,400 - AMB_RS00885 0.46 +0.4 200,446 + AMB_RS00885 0.49 -1.2 200,446 + AMB_RS00885 0.49 +0.4 200,446 + AMB_RS00885 0.49 -0.7 200,519 + AMB_RS00885 0.54 -0.7 200,636 + AMB_RS00885 0.61 +1.3 200,697 - AMB_RS00885 0.64 -0.4 200,697 - AMB_RS00885 0.64 -0.0 200,697 - AMB_RS00885 0.64 +0.2 201,242 + -1.0 201,267 - -0.7 201,284 + +1.3 201,284 + +0.6 201,307 + -1.0 201,307 + -1.2 201,308 - -0.5 201,388 + -1.0 201,388 + -1.4 201,388 + -0.4 201,388 + +0.0 201,389 - +0.1 201,389 - +0.2 201,390 + -0.4 201,390 + +0.4 201,390 + +0.6 201,391 - -1.6 201,391 - -1.2 201,442 + AMB_RS00890 0.11 +0.2 201,442 + AMB_RS00890 0.11 +0.0 201,442 + AMB_RS00890 0.11 -1.0 201,442 + AMB_RS00890 0.11 +0.5 201,443 - AMB_RS00890 0.11 -0.0 201,443 - AMB_RS00890 0.11 -1.6 201,443 - AMB_RS00890 0.11 -0.0 201,443 - AMB_RS00890 0.11 +0.7 201,511 + AMB_RS00890 0.15 +0.5 201,610 + AMB_RS00890 0.22 +1.2 201,710 - AMB_RS00890 0.29 -1.4 201,710 - AMB_RS00890 0.29 -0.2 201,772 + AMB_RS00890 0.34 -0.0 201,773 - AMB_RS00890 0.34 -1.6 201,773 - AMB_RS00890 0.34 -0.1 201,773 - AMB_RS00890 0.34 -1.4 201,943 + AMB_RS00890 0.46 -1.1 201,944 - AMB_RS00890 0.46 -1.7 202,183 + AMB_RS00890 0.63 -0.3 202,219 + AMB_RS00890 0.65 +1.9 202,219 + AMB_RS00890 0.65 +1.9 202,220 - AMB_RS00890 0.65 -0.0 202,220 - AMB_RS00890 0.65 -2.7 202,291 + AMB_RS00890 0.70 +1.1 202,291 + AMB_RS00890 0.70 -0.7 202,291 + AMB_RS00890 0.70 +0.7 202,292 - AMB_RS00890 0.70 +0.2 202,292 - AMB_RS00890 0.70 +0.0 202,591 + +0.6 202,591 + +2.2 202,592 - -1.2 202,592 - +1.7 202,592 - +0.1 202,674 + +2.0 202,697 - +0.2 202,697 - -0.7 202,712 - -0.4 202,712 - +0.2 202,712 - +0.5 202,871 + +2.1 202,871 + +1.1 202,871 + -2.1 202,871 + -0.5 202,872 - +0.2 202,872 - -1.4 202,872 - +0.6 202,872 - -1.7 202,872 - +0.0 202,883 + +0.6 202,883 + -0.2 203,135 + AMB_RS00895 0.31 +0.2 203,136 - AMB_RS00895 0.31 -0.2 203,147 + AMB_RS00895 0.32 -1.6 203,147 + AMB_RS00895 0.32 -1.2 203,189 - AMB_RS00895 0.36 -1.6 203,189 - AMB_RS00895 0.36 -1.4 203,189 - AMB_RS00895 0.36 -0.2 203,339 + AMB_RS00895 0.48 +0.2 203,340 - AMB_RS00895 0.49 -1.0 203,340 - AMB_RS00895 0.49 -1.2 203,340 - AMB_RS00895 0.49 -2.0 203,432 + AMB_RS00895 0.56 +0.0 203,432 + AMB_RS00895 0.56 +0.6 203,433 - AMB_RS00895 0.56 -1.4 203,433 - AMB_RS00895 0.56 -1.0 203,433 - AMB_RS00895 0.56 -0.8 203,654 + AMB_RS00895 0.75 -0.4
Or see this region's nucleotide sequence