Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00820 and AMB_RS00825 are separated by 36 nucleotides AMB_RS00825 and AMB_RS00830 overlap by 1 nucleotides AMB_RS00830 and AMB_RS00835 overlap by 4 nucleotides AMB_RS00835 and AMB_RS00840 are separated by 173 nucleotides
AMB_RS00820: AMB_RS00820 - GDP-L-fucose synthase, at 184,747 to 185,691
_RS00820
AMB_RS00825: AMB_RS00825 - NAD(+) kinase, at 185,728 to 186,495
_RS00825
AMB_RS00830: AMB_RS00830 - ammonia monooxygenase, at 186,495 to 187,541
_RS00830
AMB_RS00835: AMB_RS00835 - cupin fold metalloprotein, WbuC family, at 187,538 to 188,020
_RS00835
AMB_RS00840: AMB_RS00840 - DUF992 domain-containing protein, at 188,194 to 188,670
_RS00840
Position (kb)
186
187
188 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 185.668 kb on + strand at 185.668 kb on + strand at 185.669 kb on - strand at 185.689 kb on + strand at 185.689 kb on + strand at 185.689 kb on + strand at 185.689 kb on + strand at 185.689 kb on + strand at 186.798 kb on + strand, within AMB_RS00830 at 186.798 kb on + strand, within AMB_RS00830 at 186.798 kb on + strand, within AMB_RS00830 at 186.798 kb on + strand, within AMB_RS00830 at 186.799 kb on - strand, within AMB_RS00830 at 186.803 kb on - strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.909 kb on - strand, within AMB_RS00830 at 187.313 kb on - strand, within AMB_RS00830 at 187.612 kb on + strand, within AMB_RS00835 at 187.612 kb on + strand, within AMB_RS00835 at 187.613 kb on - strand, within AMB_RS00835 at 187.679 kb on + strand, within AMB_RS00835 at 187.679 kb on + strand, within AMB_RS00835 at 187.679 kb on + strand, within AMB_RS00835 at 187.679 kb on + strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.680 kb on - strand, within AMB_RS00835 at 187.682 kb on - strand, within AMB_RS00835 at 187.954 kb on + strand, within AMB_RS00835 at 188.019 kb on - strand at 188.058 kb on + strand at 188.058 kb on + strand at 188.058 kb on + strand at 188.059 kb on - strand at 188.059 kb on - strand at 188.059 kb on - strand at 188.098 kb on + strand at 188.099 kb on - strand at 188.105 kb on + strand at 188.106 kb on - strand at 188.106 kb on - strand at 188.106 kb on - strand at 188.106 kb on - strand at 188.181 kb on + strand at 188.181 kb on + strand at 188.181 kb on + strand at 188.181 kb on + strand at 188.181 kb on + strand at 188.181 kb on + strand at 188.182 kb on - strand at 188.182 kb on - strand at 188.182 kb on - strand at 188.182 kb on - strand at 188.182 kb on - strand at 188.182 kb on - strand at 188.182 kb on - strand at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.323 kb on + strand, within AMB_RS00840 at 188.324 kb on - strand, within AMB_RS00840 at 188.324 kb on - strand, within AMB_RS00840 at 188.324 kb on - strand, within AMB_RS00840 at 188.324 kb on - strand, within AMB_RS00840 at 188.324 kb on - strand, within AMB_RS00840 at 188.324 kb on - strand, within AMB_RS00840 at 188.359 kb on + strand, within AMB_RS00840 at 188.359 kb on + strand, within AMB_RS00840 at 188.360 kb on - strand, within AMB_RS00840 at 188.360 kb on - strand, within AMB_RS00840 at 188.360 kb on - strand, within AMB_RS00840 at 188.360 kb on - strand, within AMB_RS00840 at 188.387 kb on - strand, within AMB_RS00840 at 188.387 kb on - strand, within AMB_RS00840 at 188.387 kb on - strand, within AMB_RS00840 at 188.387 kb on - strand, within AMB_RS00840 at 188.452 kb on + strand, within AMB_RS00840 at 188.452 kb on + strand, within AMB_RS00840 at 188.453 kb on - strand, within AMB_RS00840 at 188.453 kb on - strand, within AMB_RS00840 at 188.503 kb on + strand, within AMB_RS00840 at 188.503 kb on + strand, within AMB_RS00840 at 188.503 kb on + strand, within AMB_RS00840 at 188.503 kb on + strand, within AMB_RS00840 at 188.503 kb on + strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840 at 188.504 kb on - strand, within AMB_RS00840
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 185,668 + +0.8 185,668 + -2.7 185,669 - -2.1 185,689 + -0.7 185,689 + -0.4 185,689 + +0.0 185,689 + -0.4 185,689 + -0.1 186,798 + AMB_RS00830 0.29 -1.9 186,798 + AMB_RS00830 0.29 -0.7 186,798 + AMB_RS00830 0.29 -1.3 186,798 + AMB_RS00830 0.29 -1.4 186,799 - AMB_RS00830 0.29 -0.2 186,803 - AMB_RS00830 0.29 +0.0 186,908 + AMB_RS00830 0.39 +0.0 186,908 + AMB_RS00830 0.39 +0.8 186,908 + AMB_RS00830 0.39 -0.4 186,908 + AMB_RS00830 0.39 +0.7 186,909 - AMB_RS00830 0.40 +0.4 187,313 - AMB_RS00830 0.78 +0.3 187,612 + AMB_RS00835 0.15 -1.0 187,612 + AMB_RS00835 0.15 -0.4 187,613 - AMB_RS00835 0.16 -1.6 187,679 + AMB_RS00835 0.29 -1.4 187,679 + AMB_RS00835 0.29 -0.9 187,679 + AMB_RS00835 0.29 +1.6 187,679 + AMB_RS00835 0.29 -1.4 187,680 - AMB_RS00835 0.29 -1.0 187,680 - AMB_RS00835 0.29 -0.2 187,680 - AMB_RS00835 0.29 +0.0 187,680 - AMB_RS00835 0.29 -0.3 187,680 - AMB_RS00835 0.29 +0.1 187,680 - AMB_RS00835 0.29 -1.4 187,680 - AMB_RS00835 0.29 +0.0 187,682 - AMB_RS00835 0.30 +0.2 187,954 + AMB_RS00835 0.86 +0.0 188,019 - +0.0 188,058 + -1.0 188,058 + -0.4 188,058 + +0.1 188,059 - +0.5 188,059 - +0.0 188,059 - +0.1 188,098 + -0.4 188,099 - -1.7 188,105 + -0.1 188,106 - -2.8 188,106 - -0.0 188,106 - +0.6 188,106 - +0.9 188,181 + -0.4 188,181 + -1.2 188,181 + -0.1 188,181 + -0.6 188,181 + -0.7 188,181 + +0.3 188,182 - -0.6 188,182 - -2.2 188,182 - -2.2 188,182 - +0.8 188,182 - -0.4 188,182 - -0.7 188,182 - -0.5 188,323 + AMB_RS00840 0.27 +0.0 188,323 + AMB_RS00840 0.27 +0.0 188,323 + AMB_RS00840 0.27 +0.6 188,323 + AMB_RS00840 0.27 +0.2 188,323 + AMB_RS00840 0.27 -1.6 188,323 + AMB_RS00840 0.27 +0.4 188,323 + AMB_RS00840 0.27 -2.9 188,323 + AMB_RS00840 0.27 -1.1 188,323 + AMB_RS00840 0.27 -0.3 188,324 - AMB_RS00840 0.27 -1.7 188,324 - AMB_RS00840 0.27 +0.3 188,324 - AMB_RS00840 0.27 -0.2 188,324 - AMB_RS00840 0.27 +0.6 188,324 - AMB_RS00840 0.27 +0.3 188,324 - AMB_RS00840 0.27 -0.1 188,359 + AMB_RS00840 0.35 -0.7 188,359 + AMB_RS00840 0.35 -1.0 188,360 - AMB_RS00840 0.35 -0.9 188,360 - AMB_RS00840 0.35 +0.2 188,360 - AMB_RS00840 0.35 +0.3 188,360 - AMB_RS00840 0.35 -2.0 188,387 - AMB_RS00840 0.40 -0.3 188,387 - AMB_RS00840 0.40 +0.1 188,387 - AMB_RS00840 0.40 -0.7 188,387 - AMB_RS00840 0.40 -0.1 188,452 + AMB_RS00840 0.54 -1.4 188,452 + AMB_RS00840 0.54 -0.7 188,453 - AMB_RS00840 0.54 +0.1 188,453 - AMB_RS00840 0.54 -0.7 188,503 + AMB_RS00840 0.65 -0.7 188,503 + AMB_RS00840 0.65 -0.7 188,503 + AMB_RS00840 0.65 +0.3 188,503 + AMB_RS00840 0.65 -0.1 188,503 + AMB_RS00840 0.65 -0.7 188,504 - AMB_RS00840 0.65 -0.4 188,504 - AMB_RS00840 0.65 -0.1 188,504 - AMB_RS00840 0.65 +0.6 188,504 - AMB_RS00840 0.65 -1.4 188,504 - AMB_RS00840 0.65 +0.4 188,504 - AMB_RS00840 0.65 -1.2 188,504 - AMB_RS00840 0.65 +0.5
Or see this region's nucleotide sequence