Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00585 and AMB_RS00590 are separated by 27 nucleotides AMB_RS00590 and AMB_RS00595 are separated by 3 nucleotides AMB_RS00595 and AMB_RS00600 are separated by 35 nucleotides
AMB_RS00585: AMB_RS00585 - hypothetical protein, at 134,161 to 134,868
_RS00585
AMB_RS00590: AMB_RS00590 - radical SAM protein, at 134,896 to 135,693
_RS00590
AMB_RS00595: AMB_RS00595 - B12-binding domain-containing radical SAM protein, at 135,697 to 137,157
_RS00595
AMB_RS00600: AMB_RS00600 - asparagine synthase (glutamine-hydrolyzing), at 137,193 to 139,112
_RS00600
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 134.708 kb on - strand, within AMB_RS00585 at 134.719 kb on + strand, within AMB_RS00585 at 134.720 kb on - strand, within AMB_RS00585 at 134.725 kb on - strand, within AMB_RS00585 at 134.725 kb on - strand, within AMB_RS00585 at 134.748 kb on + strand, within AMB_RS00585 at 134.767 kb on + strand, within AMB_RS00585 at 134.768 kb on - strand, within AMB_RS00585 at 134.768 kb on - strand, within AMB_RS00585 at 134.769 kb on + strand, within AMB_RS00585 at 134.770 kb on - strand, within AMB_RS00585 at 134.800 kb on + strand at 134.859 kb on + strand at 134.866 kb on + strand at 134.867 kb on - strand at 134.867 kb on - strand at 134.867 kb on - strand at 135.196 kb on + strand, within AMB_RS00590 at 135.196 kb on + strand, within AMB_RS00590 at 135.196 kb on + strand, within AMB_RS00590 at 135.197 kb on - strand, within AMB_RS00590 at 135.197 kb on - strand, within AMB_RS00590 at 135.201 kb on + strand, within AMB_RS00590 at 135.201 kb on + strand, within AMB_RS00590 at 135.202 kb on - strand, within AMB_RS00590 at 135.202 kb on - strand, within AMB_RS00590 at 135.229 kb on + strand, within AMB_RS00590 at 135.230 kb on - strand, within AMB_RS00590 at 135.370 kb on - strand, within AMB_RS00590 at 135.445 kb on + strand, within AMB_RS00590 at 135.445 kb on + strand, within AMB_RS00590 at 135.513 kb on + strand, within AMB_RS00590 at 135.514 kb on - strand, within AMB_RS00590 at 135.577 kb on + strand, within AMB_RS00590 at 135.578 kb on - strand, within AMB_RS00590 at 135.578 kb on - strand, within AMB_RS00590 at 135.612 kb on + strand, within AMB_RS00590 at 135.612 kb on + strand, within AMB_RS00590 at 135.612 kb on + strand, within AMB_RS00590 at 135.613 kb on - strand, within AMB_RS00590 at 135.613 kb on - strand, within AMB_RS00590 at 135.616 kb on + strand at 135.616 kb on + strand at 135.648 kb on + strand at 135.649 kb on - strand at 135.711 kb on - strand at 135.748 kb on - strand at 135.766 kb on + strand at 135.766 kb on + strand at 135.767 kb on - strand at 135.767 kb on - strand at 135.767 kb on - strand at 135.767 kb on - strand at 135.887 kb on - strand, within AMB_RS00595 at 136.036 kb on + strand, within AMB_RS00595 at 136.036 kb on + strand, within AMB_RS00595 at 136.037 kb on - strand, within AMB_RS00595 at 136.291 kb on + strand, within AMB_RS00595 at 136.292 kb on - strand, within AMB_RS00595 at 136.366 kb on + strand, within AMB_RS00595 at 136.366 kb on + strand, within AMB_RS00595 at 136.367 kb on - strand, within AMB_RS00595 at 136.367 kb on - strand, within AMB_RS00595 at 136.367 kb on - strand, within AMB_RS00595 at 136.367 kb on - strand, within AMB_RS00595 at 136.388 kb on - strand, within AMB_RS00595 at 136.498 kb on + strand, within AMB_RS00595 at 136.522 kb on + strand, within AMB_RS00595 at 136.674 kb on + strand, within AMB_RS00595 at 136.674 kb on + strand, within AMB_RS00595 at 136.678 kb on + strand, within AMB_RS00595 at 136.679 kb on - strand, within AMB_RS00595 at 136.679 kb on - strand, within AMB_RS00595 at 136.679 kb on - strand, within AMB_RS00595 at 136.868 kb on - strand, within AMB_RS00595 at 136.966 kb on + strand, within AMB_RS00595 at 136.966 kb on + strand, within AMB_RS00595 at 136.966 kb on + strand, within AMB_RS00595 at 136.967 kb on - strand, within AMB_RS00595 at 136.967 kb on - strand, within AMB_RS00595 at 137.007 kb on + strand, within AMB_RS00595 at 137.008 kb on - strand, within AMB_RS00595 at 137.039 kb on - strand at 137.053 kb on + strand at 137.155 kb on + strand at 137.156 kb on - strand at 137.156 kb on - strand at 137.175 kb on + strand at 137.175 kb on + strand at 137.175 kb on + strand at 137.176 kb on - strand at 137.176 kb on - strand at 137.176 kb on - strand at 137.192 kb on + strand at 137.192 kb on + strand at 137.192 kb on + strand at 137.192 kb on + strand at 137.192 kb on + strand at 137.193 kb on - strand at 137.193 kb on - strand at 137.193 kb on - strand at 137.193 kb on - strand at 137.193 kb on - strand at 137.409 kb on + strand, within AMB_RS00600 at 137.409 kb on + strand, within AMB_RS00600 at 137.409 kb on + strand, within AMB_RS00600 at 137.409 kb on + strand, within AMB_RS00600 at 137.409 kb on + strand, within AMB_RS00600 at 137.410 kb on - strand, within AMB_RS00600 at 137.410 kb on - strand, within AMB_RS00600 at 137.556 kb on + strand, within AMB_RS00600 at 137.556 kb on + strand, within AMB_RS00600 at 137.557 kb on - strand, within AMB_RS00600 at 137.593 kb on + strand, within AMB_RS00600 at 137.631 kb on + strand, within AMB_RS00600 at 137.739 kb on + strand, within AMB_RS00600 at 137.739 kb on + strand, within AMB_RS00600 at 137.740 kb on - strand, within AMB_RS00600 at 137.740 kb on - strand, within AMB_RS00600 at 137.740 kb on - strand, within AMB_RS00600 at 137.740 kb on - strand, within AMB_RS00600 at 137.740 kb on - strand, within AMB_RS00600 at 137.745 kb on + strand, within AMB_RS00600 at 137.745 kb on + strand, within AMB_RS00600 at 137.745 kb on + strand, within AMB_RS00600 at 137.746 kb on - strand, within AMB_RS00600 at 137.746 kb on - strand, within AMB_RS00600 at 137.746 kb on - strand, within AMB_RS00600 at 137.746 kb on - strand, within AMB_RS00600 at 137.746 kb on - strand, within AMB_RS00600 at 137.747 kb on + strand, within AMB_RS00600 at 137.853 kb on + strand, within AMB_RS00600 at 138.012 kb on + strand, within AMB_RS00600 at 138.012 kb on + strand, within AMB_RS00600 at 138.012 kb on + strand, within AMB_RS00600 at 138.013 kb on - strand, within AMB_RS00600 at 138.015 kb on + strand, within AMB_RS00600 at 138.131 kb on + strand, within AMB_RS00600 at 138.132 kb on - strand, within AMB_RS00600 at 138.132 kb on - strand, within AMB_RS00600 at 138.132 kb on - strand, within AMB_RS00600
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 134,708 - AMB_RS00585 0.77 -0.2 134,719 + AMB_RS00585 0.79 -0.4 134,720 - AMB_RS00585 0.79 +0.4 134,725 - AMB_RS00585 0.80 -0.2 134,725 - AMB_RS00585 0.80 -1.2 134,748 + AMB_RS00585 0.83 -0.3 134,767 + AMB_RS00585 0.86 +0.1 134,768 - AMB_RS00585 0.86 +0.1 134,768 - AMB_RS00585 0.86 -0.7 134,769 + AMB_RS00585 0.86 -1.2 134,770 - AMB_RS00585 0.86 +0.7 134,800 + +0.4 134,859 + +0.0 134,866 + +1.6 134,867 - -1.6 134,867 - +0.6 134,867 - -1.2 135,196 + AMB_RS00590 0.38 -0.1 135,196 + AMB_RS00590 0.38 -3.0 135,196 + AMB_RS00590 0.38 +1.6 135,197 - AMB_RS00590 0.38 +0.6 135,197 - AMB_RS00590 0.38 -0.1 135,201 + AMB_RS00590 0.38 +0.5 135,201 + AMB_RS00590 0.38 -0.1 135,202 - AMB_RS00590 0.38 -0.7 135,202 - AMB_RS00590 0.38 -0.4 135,229 + AMB_RS00590 0.42 -0.7 135,230 - AMB_RS00590 0.42 -1.0 135,370 - AMB_RS00590 0.59 +0.0 135,445 + AMB_RS00590 0.69 -1.7 135,445 + AMB_RS00590 0.69 -1.6 135,513 + AMB_RS00590 0.77 -0.1 135,514 - AMB_RS00590 0.77 +0.5 135,577 + AMB_RS00590 0.85 -0.1 135,578 - AMB_RS00590 0.85 -0.4 135,578 - AMB_RS00590 0.85 -1.9 135,612 + AMB_RS00590 0.90 +1.8 135,612 + AMB_RS00590 0.90 -1.0 135,612 + AMB_RS00590 0.90 +0.2 135,613 - AMB_RS00590 0.90 +0.0 135,613 - AMB_RS00590 0.90 +0.8 135,616 + -1.4 135,616 + +0.6 135,648 + -0.7 135,649 - -1.0 135,711 - -0.2 135,748 - -1.7 135,766 + -0.4 135,766 + +0.5 135,767 - -1.0 135,767 - +0.1 135,767 - +0.1 135,767 - -1.0 135,887 - AMB_RS00595 0.13 +1.6 136,036 + AMB_RS00595 0.23 -1.0 136,036 + AMB_RS00595 0.23 -1.7 136,037 - AMB_RS00595 0.23 -0.9 136,291 + AMB_RS00595 0.41 -1.7 136,292 - AMB_RS00595 0.41 -0.4 136,366 + AMB_RS00595 0.46 +0.3 136,366 + AMB_RS00595 0.46 +0.9 136,367 - AMB_RS00595 0.46 +0.6 136,367 - AMB_RS00595 0.46 +0.0 136,367 - AMB_RS00595 0.46 +0.0 136,367 - AMB_RS00595 0.46 -1.4 136,388 - AMB_RS00595 0.47 +0.1 136,498 + AMB_RS00595 0.55 -0.1 136,522 + AMB_RS00595 0.56 +0.2 136,674 + AMB_RS00595 0.67 -0.7 136,674 + AMB_RS00595 0.67 +0.0 136,678 + AMB_RS00595 0.67 -1.6 136,679 - AMB_RS00595 0.67 +0.1 136,679 - AMB_RS00595 0.67 +0.0 136,679 - AMB_RS00595 0.67 -1.0 136,868 - AMB_RS00595 0.80 +0.8 136,966 + AMB_RS00595 0.87 -1.4 136,966 + AMB_RS00595 0.87 -0.1 136,966 + AMB_RS00595 0.87 -1.0 136,967 - AMB_RS00595 0.87 -2.4 136,967 - AMB_RS00595 0.87 -0.4 137,007 + AMB_RS00595 0.90 +0.4 137,008 - AMB_RS00595 0.90 +0.4 137,039 - -0.4 137,053 + +0.2 137,155 + -1.7 137,156 - -0.5 137,156 - -0.4 137,175 + +0.3 137,175 + -1.6 137,175 + -1.0 137,176 - -2.4 137,176 - -0.7 137,176 - -0.1 137,192 + +0.6 137,192 + -0.1 137,192 + +0.0 137,192 + -0.2 137,192 + -0.7 137,193 - -0.5 137,193 - -2.5 137,193 - -0.4 137,193 - -1.0 137,193 - -0.5 137,409 + AMB_RS00600 0.11 +0.1 137,409 + AMB_RS00600 0.11 -1.4 137,409 + AMB_RS00600 0.11 -1.7 137,409 + AMB_RS00600 0.11 -0.2 137,409 + AMB_RS00600 0.11 -0.4 137,410 - AMB_RS00600 0.11 -3.0 137,410 - AMB_RS00600 0.11 +0.2 137,556 + AMB_RS00600 0.19 +0.1 137,556 + AMB_RS00600 0.19 +0.5 137,557 - AMB_RS00600 0.19 +0.6 137,593 + AMB_RS00600 0.21 +0.7 137,631 + AMB_RS00600 0.23 -2.0 137,739 + AMB_RS00600 0.28 +1.2 137,739 + AMB_RS00600 0.28 -0.7 137,740 - AMB_RS00600 0.28 -0.4 137,740 - AMB_RS00600 0.28 +0.0 137,740 - AMB_RS00600 0.28 +0.1 137,740 - AMB_RS00600 0.28 +0.3 137,740 - AMB_RS00600 0.28 +0.4 137,745 + AMB_RS00600 0.29 +0.5 137,745 + AMB_RS00600 0.29 -3.6 137,745 + AMB_RS00600 0.29 -0.4 137,746 - AMB_RS00600 0.29 -0.2 137,746 - AMB_RS00600 0.29 +0.2 137,746 - AMB_RS00600 0.29 -1.6 137,746 - AMB_RS00600 0.29 +0.1 137,746 - AMB_RS00600 0.29 -0.5 137,747 + AMB_RS00600 0.29 -1.2 137,853 + AMB_RS00600 0.34 -1.9 138,012 + AMB_RS00600 0.43 +0.2 138,012 + AMB_RS00600 0.43 -0.6 138,012 + AMB_RS00600 0.43 +0.3 138,013 - AMB_RS00600 0.43 -0.8 138,015 + AMB_RS00600 0.43 +0.1 138,131 + AMB_RS00600 0.49 -0.4 138,132 - AMB_RS00600 0.49 -0.4 138,132 - AMB_RS00600 0.49 +0.1 138,132 - AMB_RS00600 0.49 +0.1
Or see this region's nucleotide sequence