Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00345 and AMB_RS00350 overlap by 1 nucleotides AMB_RS00350 and AMB_RS00355 overlap by 25 nucleotides AMB_RS00355 and AMB_RS00360 are separated by 9 nucleotides AMB_RS00360 and AMB_RS23815 overlap by 4 nucleotides
AMB_RS00345: AMB_RS00345 - hypothetical protein, at 74,922 to 75,731
_RS00345
AMB_RS00350: AMB_RS00350 - hypothetical protein, at 75,731 to 76,135
_RS00350
AMB_RS00355: AMB_RS00355 - radical SAM protein, at 76,111 to 77,175
_RS00355
AMB_RS00360: AMB_RS00360 - class I SAM-dependent methyltransferase, at 77,185 to 77,847
_RS00360
AMB_RS23815: AMB_RS23815 - TIGR04372 family glycosyltransferase, at 77,844 to 79,100
_RS23815
Position (kb) 76
77
78 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 76.095 kb on + strand at 76.096 kb on - strand at 76.123 kb on + strand at 76.233 kb on + strand, within AMB_RS00355 at 76.233 kb on + strand, within AMB_RS00355 at 76.234 kb on - strand, within AMB_RS00355 at 76.269 kb on + strand, within AMB_RS00355 at 76.269 kb on + strand, within AMB_RS00355 at 76.269 kb on + strand, within AMB_RS00355 at 76.270 kb on - strand, within AMB_RS00355 at 76.397 kb on - strand, within AMB_RS00355 at 76.397 kb on - strand, within AMB_RS00355 at 76.555 kb on + strand, within AMB_RS00355 at 76.555 kb on + strand, within AMB_RS00355 at 76.555 kb on + strand, within AMB_RS00355 at 76.556 kb on - strand, within AMB_RS00355 at 76.556 kb on - strand, within AMB_RS00355 at 76.599 kb on - strand, within AMB_RS00355 at 76.608 kb on + strand, within AMB_RS00355 at 76.608 kb on + strand, within AMB_RS00355 at 76.635 kb on + strand, within AMB_RS00355 at 76.687 kb on - strand, within AMB_RS00355 at 76.781 kb on + strand, within AMB_RS00355 at 76.781 kb on + strand, within AMB_RS00355 at 76.782 kb on - strand, within AMB_RS00355 at 76.782 kb on - strand, within AMB_RS00355 at 76.870 kb on + strand, within AMB_RS00355 at 76.903 kb on + strand, within AMB_RS00355 at 76.905 kb on + strand, within AMB_RS00355 at 76.962 kb on + strand, within AMB_RS00355 at 76.962 kb on + strand, within AMB_RS00355 at 76.962 kb on + strand, within AMB_RS00355 at 76.962 kb on + strand, within AMB_RS00355 at 76.962 kb on + strand, within AMB_RS00355 at 76.962 kb on + strand, within AMB_RS00355 at 76.963 kb on - strand, within AMB_RS00355 at 76.963 kb on - strand, within AMB_RS00355 at 76.981 kb on + strand, within AMB_RS00355 at 76.981 kb on + strand, within AMB_RS00355 at 76.981 kb on + strand, within AMB_RS00355 at 76.983 kb on + strand, within AMB_RS00355 at 76.983 kb on + strand, within AMB_RS00355 at 77.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 76,095 + -2.0 76,096 - +0.1 76,123 + -2.7 76,233 + AMB_RS00355 0.11 -1.0 76,233 + AMB_RS00355 0.11 -0.7 76,234 - AMB_RS00355 0.12 -1.4 76,269 + AMB_RS00355 0.15 -3.1 76,269 + AMB_RS00355 0.15 -6.9 76,269 + AMB_RS00355 0.15 -5.0 76,270 - AMB_RS00355 0.15 -4.1 76,397 - AMB_RS00355 0.27 -1.0 76,397 - AMB_RS00355 0.27 -1.6 76,555 + AMB_RS00355 0.42 +0.6 76,555 + AMB_RS00355 0.42 -5.1 76,555 + AMB_RS00355 0.42 -6.6 76,556 - AMB_RS00355 0.42 +0.0 76,556 - AMB_RS00355 0.42 -2.0 76,599 - AMB_RS00355 0.46 +0.0 76,608 + AMB_RS00355 0.47 -1.2 76,608 + AMB_RS00355 0.47 -2.7 76,635 + AMB_RS00355 0.49 -1.4 76,687 - AMB_RS00355 0.54 +0.3 76,781 + AMB_RS00355 0.63 -2.5 76,781 + AMB_RS00355 0.63 -4.8 76,782 - AMB_RS00355 0.63 -4.5 76,782 - AMB_RS00355 0.63 -1.9 76,870 + AMB_RS00355 0.71 -2.2 76,903 + AMB_RS00355 0.74 -4.4 76,905 + AMB_RS00355 0.75 +0.6 76,962 + AMB_RS00355 0.80 -0.9 76,962 + AMB_RS00355 0.80 -4.3 76,962 + AMB_RS00355 0.80 -0.4 76,962 + AMB_RS00355 0.80 +0.6 76,962 + AMB_RS00355 0.80 -0.7 76,962 + AMB_RS00355 0.80 +0.0 76,963 - AMB_RS00355 0.80 -1.0 76,963 - AMB_RS00355 0.80 -4.5 76,981 + AMB_RS00355 0.82 -3.3 76,981 + AMB_RS00355 0.82 -1.0 76,981 + AMB_RS00355 0.82 -4.8 76,983 + AMB_RS00355 0.82 -1.0 76,983 + AMB_RS00355 0.82 -0.4 77,137 + +1.6
Or see this region's nucleotide sequence