Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00170 and AMB_RS00175 overlap by 44 nucleotides AMB_RS00175 and AMB_RS00180 are separated by 171 nucleotides AMB_RS00180 and AMB_RS00185 are separated by 23 nucleotides
AMB_RS00170: AMB_RS00170 - phosphoheptose isomerase, at 37,904 to 38,482
_RS00170
AMB_RS00175: AMB_RS00175 - hypothetical protein, at 38,439 to 38,987
_RS00175
AMB_RS00180: AMB_RS00180 - hypothetical protein, at 39,159 to 40,997
_RS00180
AMB_RS00185: AMB_RS00185 - hypothetical protein, at 41,021 to 42,601
_RS00185
Position (kb) 39
40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 38.512 kb on + strand, within AMB_RS00175 at 38.528 kb on + strand, within AMB_RS00175 at 38.528 kb on + strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.928 kb on + strand, within AMB_RS00175 at 38.928 kb on + strand, within AMB_RS00175 at 38.985 kb on + strand at 38.985 kb on + strand at 38.986 kb on - strand at 39.134 kb on + strand at 39.134 kb on + strand at 39.134 kb on + strand at 39.146 kb on + strand at 39.186 kb on + strand at 39.186 kb on + strand at 39.300 kb on + strand at 39.354 kb on + strand, within AMB_RS00180 at 39.396 kb on + strand, within AMB_RS00180 at 39.397 kb on - strand, within AMB_RS00180 at 39.397 kb on - strand, within AMB_RS00180 at 39.720 kb on + strand, within AMB_RS00180 at 39.721 kb on - strand, within AMB_RS00180 at 39.721 kb on - strand, within AMB_RS00180 at 39.735 kb on + strand, within AMB_RS00180 at 39.735 kb on + strand, within AMB_RS00180 at 39.735 kb on + strand, within AMB_RS00180 at 39.768 kb on + strand, within AMB_RS00180 at 39.768 kb on + strand, within AMB_RS00180 at 39.768 kb on + strand, within AMB_RS00180 at 39.792 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.886 kb on - strand, within AMB_RS00180 at 39.998 kb on + strand, within AMB_RS00180 at 40.145 kb on + strand, within AMB_RS00180 at 40.145 kb on + strand, within AMB_RS00180 at 40.596 kb on + strand, within AMB_RS00180 at 40.597 kb on - strand, within AMB_RS00180 at 40.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 38,512 + AMB_RS00175 0.13 -0.4 38,528 + AMB_RS00175 0.16 -0.1 38,528 + AMB_RS00175 0.16 +0.0 38,529 - AMB_RS00175 0.16 -0.4 38,529 - AMB_RS00175 0.16 -1.0 38,529 - AMB_RS00175 0.16 -2.5 38,529 - AMB_RS00175 0.16 -0.4 38,928 + AMB_RS00175 0.89 -2.1 38,928 + AMB_RS00175 0.89 +0.6 38,985 + +0.0 38,985 + -0.4 38,986 - -0.4 39,134 + -0.1 39,134 + -0.5 39,134 + -3.3 39,146 + +0.0 39,186 + +0.0 39,186 + -0.4 39,300 + -0.5 39,354 + AMB_RS00180 0.11 -0.7 39,396 + AMB_RS00180 0.13 -1.0 39,397 - AMB_RS00180 0.13 -2.0 39,397 - AMB_RS00180 0.13 -0.7 39,720 + AMB_RS00180 0.31 -1.6 39,721 - AMB_RS00180 0.31 -1.5 39,721 - AMB_RS00180 0.31 -1.0 39,735 + AMB_RS00180 0.31 -0.1 39,735 + AMB_RS00180 0.31 -0.7 39,735 + AMB_RS00180 0.31 -2.9 39,768 + AMB_RS00180 0.33 -1.7 39,768 + AMB_RS00180 0.33 -1.2 39,768 + AMB_RS00180 0.33 -2.7 39,792 + AMB_RS00180 0.34 -0.4 39,876 + AMB_RS00180 0.39 -1.7 39,876 + AMB_RS00180 0.39 -0.7 39,876 + AMB_RS00180 0.39 -1.6 39,876 + AMB_RS00180 0.39 -1.0 39,886 - AMB_RS00180 0.40 -3.4 39,998 + AMB_RS00180 0.46 +0.1 40,145 + AMB_RS00180 0.54 -0.6 40,145 + AMB_RS00180 0.54 -1.0 40,596 + AMB_RS00180 0.78 -1.4 40,597 - AMB_RS00180 0.78 -1.0 40,971 + -1.2
Or see this region's nucleotide sequence