Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00165 and AMB_RS00170 are separated by 32 nucleotides AMB_RS00170 and AMB_RS00175 overlap by 44 nucleotides AMB_RS00175 and AMB_RS00180 are separated by 171 nucleotides
AMB_RS00165: AMB_RS00165 - NAD-glutamate dehydrogenase, at 33,060 to 37,871
_RS00165
AMB_RS00170: AMB_RS00170 - phosphoheptose isomerase, at 37,904 to 38,482
_RS00170
AMB_RS00175: AMB_RS00175 - hypothetical protein, at 38,439 to 38,987
_RS00175
AMB_RS00180: AMB_RS00180 - hypothetical protein, at 39,159 to 40,997
_RS00180
Position (kb) 38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 37.521 kb on + strand at 37.635 kb on + strand at 37.678 kb on - strand at 37.678 kb on - strand at 38.512 kb on + strand, within AMB_RS00175 at 38.528 kb on + strand, within AMB_RS00175 at 38.528 kb on + strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.529 kb on - strand, within AMB_RS00175 at 38.928 kb on + strand, within AMB_RS00175 at 38.928 kb on + strand, within AMB_RS00175 at 38.985 kb on + strand at 38.985 kb on + strand at 38.986 kb on - strand at 39.134 kb on + strand at 39.134 kb on + strand at 39.134 kb on + strand at 39.146 kb on + strand at 39.186 kb on + strand at 39.186 kb on + strand at 39.300 kb on + strand at 39.354 kb on + strand, within AMB_RS00180 at 39.396 kb on + strand, within AMB_RS00180 at 39.397 kb on - strand, within AMB_RS00180 at 39.397 kb on - strand, within AMB_RS00180 at 39.720 kb on + strand, within AMB_RS00180 at 39.721 kb on - strand, within AMB_RS00180 at 39.721 kb on - strand, within AMB_RS00180 at 39.735 kb on + strand, within AMB_RS00180 at 39.735 kb on + strand, within AMB_RS00180 at 39.735 kb on + strand, within AMB_RS00180 at 39.768 kb on + strand, within AMB_RS00180 at 39.768 kb on + strand, within AMB_RS00180 at 39.768 kb on + strand, within AMB_RS00180 at 39.792 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.876 kb on + strand, within AMB_RS00180 at 39.886 kb on - strand, within AMB_RS00180
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 37,521 + +0.6 37,635 + +0.5 37,678 - +0.0 37,678 - +0.0 38,512 + AMB_RS00175 0.13 -0.4 38,528 + AMB_RS00175 0.16 -0.1 38,528 + AMB_RS00175 0.16 +0.0 38,529 - AMB_RS00175 0.16 -0.4 38,529 - AMB_RS00175 0.16 -1.0 38,529 - AMB_RS00175 0.16 -2.5 38,529 - AMB_RS00175 0.16 -0.4 38,928 + AMB_RS00175 0.89 -2.1 38,928 + AMB_RS00175 0.89 +0.6 38,985 + +0.0 38,985 + -0.4 38,986 - -0.4 39,134 + -0.1 39,134 + -0.5 39,134 + -3.3 39,146 + +0.0 39,186 + +0.0 39,186 + -0.4 39,300 + -0.5 39,354 + AMB_RS00180 0.11 -0.7 39,396 + AMB_RS00180 0.13 -1.0 39,397 - AMB_RS00180 0.13 -2.0 39,397 - AMB_RS00180 0.13 -0.7 39,720 + AMB_RS00180 0.31 -1.6 39,721 - AMB_RS00180 0.31 -1.5 39,721 - AMB_RS00180 0.31 -1.0 39,735 + AMB_RS00180 0.31 -0.1 39,735 + AMB_RS00180 0.31 -0.7 39,735 + AMB_RS00180 0.31 -2.9 39,768 + AMB_RS00180 0.33 -1.7 39,768 + AMB_RS00180 0.33 -1.2 39,768 + AMB_RS00180 0.33 -2.7 39,792 + AMB_RS00180 0.34 -0.4 39,876 + AMB_RS00180 0.39 -1.7 39,876 + AMB_RS00180 0.39 -0.7 39,876 + AMB_RS00180 0.39 -1.6 39,876 + AMB_RS00180 0.39 -1.0 39,886 - AMB_RS00180 0.40 -3.4
Or see this region's nucleotide sequence