Experiment: Choline (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_05069 and PFR28_05070 are separated by 335 nucleotides PFR28_05070 and PFR28_05071 overlap by 17 nucleotides
PFR28_05069: PFR28_05069 - hypothetical protein, at 5,805,401 to 5,806,855
_05069
PFR28_05070: PFR28_05070 - nebramycin 5, at 5,807,191 to 5,808,948
_05070
PFR28_05071: PFR28_05071 - D-inositol-3-phosphate glycosyltransferase, at 5,808,932 to 5,810,062
_05071
Position (kb)
5807
5808
5809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5806.854 kb on - strand at 5806.865 kb on + strand at 5807.067 kb on + strand at 5807.075 kb on + strand at 5807.076 kb on - strand at 5807.076 kb on - strand at 5807.087 kb on + strand at 5807.087 kb on + strand at 5807.088 kb on - strand at 5807.092 kb on + strand at 5807.092 kb on + strand at 5807.092 kb on + strand at 5807.092 kb on + strand at 5807.092 kb on + strand at 5807.093 kb on - strand at 5807.107 kb on + strand at 5807.107 kb on + strand at 5807.174 kb on + strand at 5807.174 kb on + strand at 5807.174 kb on + strand at 5807.255 kb on + strand at 5808.107 kb on + strand, within PFR28_05070 at 5808.107 kb on + strand, within PFR28_05070 at 5808.107 kb on + strand, within PFR28_05070 at 5808.256 kb on + strand, within PFR28_05070 at 5808.745 kb on + strand, within PFR28_05070 at 5808.745 kb on + strand, within PFR28_05070 at 5808.865 kb on + strand at 5808.865 kb on + strand at 5808.865 kb on + strand at 5808.865 kb on + strand at 5808.883 kb on + strand at 5808.883 kb on + strand at 5809.124 kb on + strand, within PFR28_05071 at 5809.235 kb on + strand, within PFR28_05071 at 5809.235 kb on + strand, within PFR28_05071 at 5809.331 kb on + strand, within PFR28_05071 at 5809.788 kb on + strand, within PFR28_05071 at 5809.900 kb on + strand, within PFR28_05071 at 5809.900 kb on + strand, within PFR28_05071
Per-strain Table
Position Strand Gene LocusTag Fraction Choline (C) 10 mM remove 5,806,854 - -1.8 5,806,865 + +0.5 5,807,067 + -3.0 5,807,075 + -0.3 5,807,076 - +1.1 5,807,076 - +2.0 5,807,087 + +0.9 5,807,087 + +0.4 5,807,088 - -0.9 5,807,092 + +0.2 5,807,092 + -1.5 5,807,092 + -1.8 5,807,092 + +0.3 5,807,092 + +1.3 5,807,093 - +0.7 5,807,107 + +0.2 5,807,107 + +0.7 5,807,174 + +1.3 5,807,174 + -0.9 5,807,174 + -0.9 5,807,255 + +0.7 5,808,107 + PFR28_05070 0.52 -0.3 5,808,107 + PFR28_05070 0.52 +1.7 5,808,107 + PFR28_05070 0.52 -1.6 5,808,256 + PFR28_05070 0.61 -1.1 5,808,745 + PFR28_05070 0.88 -1.5 5,808,745 + PFR28_05070 0.88 +2.1 5,808,865 + +1.0 5,808,865 + -1.3 5,808,865 + +0.2 5,808,865 + -2.6 5,808,883 + -1.6 5,808,883 + -1.3 5,809,124 + PFR28_05071 0.17 -2.7 5,809,235 + PFR28_05071 0.27 -2.3 5,809,235 + PFR28_05071 0.27 -1.8 5,809,331 + PFR28_05071 0.35 -3.3 5,809,788 + PFR28_05071 0.76 -2.5 5,809,900 + PFR28_05071 0.86 -1.9 5,809,900 + PFR28_05071 0.86 -2.1
Or see this region's nucleotide sequence