Experiment: Choline (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_01109 and PFR28_01110 are separated by 526 nucleotides PFR28_01110 and PFR28_01111 are separated by 30 nucleotides
PFR28_01109: PFR28_01109 - hypothetical protein, at 1,225,573 to 1,226,010
_01109
PFR28_01110: PFR28_01110 - Fatty acid oxidation complex subunit alpha, at 1,226,537 to 1,228,684
_01110
PFR28_01111: PFR28_01111 - 3-ketoacyl-CoA thiolase, at 1,228,715 to 1,229,890
_01111
Position (kb)
1226
1227
1228
1229 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1225.566 kb on + strand at 1225.566 kb on + strand at 1225.567 kb on - strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.572 kb on + strand at 1225.573 kb on - strand at 1225.573 kb on - strand at 1225.573 kb on - strand at 1225.573 kb on - strand at 1225.646 kb on + strand, within PFR28_01109 at 1225.647 kb on - strand, within PFR28_01109 at 1225.765 kb on + strand, within PFR28_01109 at 1225.765 kb on + strand, within PFR28_01109 at 1225.807 kb on + strand, within PFR28_01109 at 1225.808 kb on - strand, within PFR28_01109 at 1225.906 kb on + strand, within PFR28_01109 at 1225.906 kb on + strand, within PFR28_01109 at 1225.906 kb on + strand, within PFR28_01109 at 1225.907 kb on - strand, within PFR28_01109 at 1225.907 kb on - strand, within PFR28_01109 at 1225.942 kb on + strand, within PFR28_01109 at 1225.942 kb on + strand, within PFR28_01109 at 1225.942 kb on + strand, within PFR28_01109 at 1225.943 kb on - strand, within PFR28_01109 at 1225.970 kb on + strand at 1225.970 kb on + strand at 1225.970 kb on + strand at 1225.971 kb on - strand at 1225.971 kb on - strand at 1226.053 kb on - strand at 1226.053 kb on - strand at 1226.100 kb on - strand at 1226.164 kb on - strand at 1226.164 kb on - strand at 1226.164 kb on - strand at 1226.164 kb on - strand at 1226.164 kb on - strand at 1226.174 kb on - strand at 1226.220 kb on + strand at 1226.221 kb on - strand at 1226.221 kb on - strand at 1226.229 kb on + strand at 1226.229 kb on + strand at 1226.229 kb on + strand at 1226.230 kb on - strand at 1226.230 kb on - strand at 1226.311 kb on + strand at 1226.353 kb on - strand at 1226.371 kb on + strand at 1226.371 kb on + strand at 1226.372 kb on - strand at 1226.372 kb on - strand at 1226.405 kb on + strand at 1226.406 kb on - strand at 1226.406 kb on - strand at 1226.453 kb on + strand at 1226.453 kb on + strand at 1226.453 kb on + strand at 1226.454 kb on - strand at 1226.454 kb on - strand at 1226.503 kb on + strand at 1226.503 kb on + strand at 1226.503 kb on + strand at 1226.504 kb on - strand at 1226.504 kb on - strand at 1226.504 kb on - strand at 1226.504 kb on - strand at 1226.543 kb on + strand at 1226.551 kb on + strand at 1226.674 kb on + strand at 1226.674 kb on + strand at 1226.675 kb on - strand at 1226.818 kb on + strand, within PFR28_01110 at 1226.972 kb on + strand, within PFR28_01110 at 1227.170 kb on + strand, within PFR28_01110 at 1227.171 kb on - strand, within PFR28_01110 at 1227.527 kb on + strand, within PFR28_01110 at 1227.698 kb on + strand, within PFR28_01110 at 1227.699 kb on - strand, within PFR28_01110 at 1227.699 kb on - strand, within PFR28_01110 at 1227.977 kb on + strand, within PFR28_01110 at 1228.262 kb on + strand, within PFR28_01110 at 1228.262 kb on + strand, within PFR28_01110 at 1228.262 kb on + strand, within PFR28_01110 at 1228.304 kb on + strand, within PFR28_01110 at 1228.304 kb on + strand, within PFR28_01110 at 1228.304 kb on + strand, within PFR28_01110 at 1228.304 kb on + strand, within PFR28_01110 at 1228.304 kb on + strand, within PFR28_01110 at 1228.304 kb on + strand, within PFR28_01110 at 1228.305 kb on - strand, within PFR28_01110 at 1228.514 kb on + strand at 1228.514 kb on + strand at 1228.514 kb on + strand at 1228.556 kb on + strand at 1228.625 kb on + strand at 1228.625 kb on + strand at 1228.625 kb on + strand at 1228.697 kb on + strand at 1228.714 kb on + strand at 1228.714 kb on + strand at 1228.714 kb on + strand at 1228.715 kb on - strand at 1228.715 kb on - strand at 1228.715 kb on - strand at 1228.715 kb on - strand at 1228.715 kb on - strand at 1228.715 kb on - strand at 1228.715 kb on - strand at 1228.730 kb on - strand at 1229.131 kb on + strand, within PFR28_01111 at 1229.131 kb on + strand, within PFR28_01111 at 1229.131 kb on + strand, within PFR28_01111 at 1229.131 kb on + strand, within PFR28_01111 at 1229.131 kb on + strand, within PFR28_01111 at 1229.132 kb on - strand, within PFR28_01111 at 1229.132 kb on - strand, within PFR28_01111 at 1229.132 kb on - strand, within PFR28_01111 at 1229.141 kb on + strand, within PFR28_01111 at 1229.141 kb on + strand, within PFR28_01111 at 1229.142 kb on - strand, within PFR28_01111 at 1229.309 kb on + strand, within PFR28_01111 at 1229.343 kb on - strand, within PFR28_01111 at 1229.570 kb on + strand, within PFR28_01111
Per-strain Table
Position Strand Gene LocusTag Fraction Choline (C) 10 mM remove 1,225,566 + -1.5 1,225,566 + +0.1 1,225,567 - -1.3 1,225,572 + +0.7 1,225,572 + +0.4 1,225,572 + +0.3 1,225,572 + -0.3 1,225,572 + +0.1 1,225,572 + -0.8 1,225,572 + -0.4 1,225,572 + -0.8 1,225,572 + +1.9 1,225,572 + +1.0 1,225,572 + -0.5 1,225,572 + -0.6 1,225,573 - -0.1 1,225,573 - -0.8 1,225,573 - +1.0 1,225,573 - -2.7 1,225,646 + PFR28_01109 0.17 +0.3 1,225,647 - PFR28_01109 0.17 -1.1 1,225,765 + PFR28_01109 0.44 +1.7 1,225,765 + PFR28_01109 0.44 -1.9 1,225,807 + PFR28_01109 0.53 +1.5 1,225,808 - PFR28_01109 0.54 +0.1 1,225,906 + PFR28_01109 0.76 -0.4 1,225,906 + PFR28_01109 0.76 +0.2 1,225,906 + PFR28_01109 0.76 +0.2 1,225,907 - PFR28_01109 0.76 +0.9 1,225,907 - PFR28_01109 0.76 +0.9 1,225,942 + PFR28_01109 0.84 +1.2 1,225,942 + PFR28_01109 0.84 +0.4 1,225,942 + PFR28_01109 0.84 +0.5 1,225,943 - PFR28_01109 0.84 -0.8 1,225,970 + +2.8 1,225,970 + +0.7 1,225,970 + -0.5 1,225,971 - +0.3 1,225,971 - -0.7 1,226,053 - +0.6 1,226,053 - -0.7 1,226,100 - +0.6 1,226,164 - +0.0 1,226,164 - +0.5 1,226,164 - -0.8 1,226,164 - +1.5 1,226,164 - -0.1 1,226,174 - -0.2 1,226,220 + -0.1 1,226,221 - +1.3 1,226,221 - +2.0 1,226,229 + -0.8 1,226,229 + +0.2 1,226,229 + +0.3 1,226,230 - -2.1 1,226,230 - -0.8 1,226,311 + -1.5 1,226,353 - +1.5 1,226,371 + -0.7 1,226,371 + -0.8 1,226,372 - -1.1 1,226,372 - +0.3 1,226,405 + +0.8 1,226,406 - -1.2 1,226,406 - -1.7 1,226,453 + +0.2 1,226,453 + +1.3 1,226,453 + +0.5 1,226,454 - +0.2 1,226,454 - +1.0 1,226,503 + -3.2 1,226,503 + +1.1 1,226,503 + -0.4 1,226,504 - -2.1 1,226,504 - +1.8 1,226,504 - -1.2 1,226,504 - +0.8 1,226,543 + -1.8 1,226,551 + +2.1 1,226,674 + +0.6 1,226,674 + -2.6 1,226,675 - -3.1 1,226,818 + PFR28_01110 0.13 -1.7 1,226,972 + PFR28_01110 0.20 +0.1 1,227,170 + PFR28_01110 0.29 -2.3 1,227,171 - PFR28_01110 0.30 -1.5 1,227,527 + PFR28_01110 0.46 -2.3 1,227,698 + PFR28_01110 0.54 +1.2 1,227,699 - PFR28_01110 0.54 -1.1 1,227,699 - PFR28_01110 0.54 -1.9 1,227,977 + PFR28_01110 0.67 -1.3 1,228,262 + PFR28_01110 0.80 -0.7 1,228,262 + PFR28_01110 0.80 +1.3 1,228,262 + PFR28_01110 0.80 +0.1 1,228,304 + PFR28_01110 0.82 +0.4 1,228,304 + PFR28_01110 0.82 +0.1 1,228,304 + PFR28_01110 0.82 -0.5 1,228,304 + PFR28_01110 0.82 -2.5 1,228,304 + PFR28_01110 0.82 -1.2 1,228,304 + PFR28_01110 0.82 -1.3 1,228,305 - PFR28_01110 0.82 -0.2 1,228,514 + -0.5 1,228,514 + -3.8 1,228,514 + +0.5 1,228,556 + +1.7 1,228,625 + +0.1 1,228,625 + -0.7 1,228,625 + -2.7 1,228,697 + -0.8 1,228,714 + -2.4 1,228,714 + -0.7 1,228,714 + -2.3 1,228,715 - +0.8 1,228,715 - +0.3 1,228,715 - -1.2 1,228,715 - -0.7 1,228,715 - -1.3 1,228,715 - +0.9 1,228,715 - -2.1 1,228,730 - +0.5 1,229,131 + PFR28_01111 0.35 +0.6 1,229,131 + PFR28_01111 0.35 -2.6 1,229,131 + PFR28_01111 0.35 +0.4 1,229,131 + PFR28_01111 0.35 -0.7 1,229,131 + PFR28_01111 0.35 -2.7 1,229,132 - PFR28_01111 0.35 -1.8 1,229,132 - PFR28_01111 0.35 +0.2 1,229,132 - PFR28_01111 0.35 +1.1 1,229,141 + PFR28_01111 0.36 -0.6 1,229,141 + PFR28_01111 0.36 -0.0 1,229,142 - PFR28_01111 0.36 -0.7 1,229,309 + PFR28_01111 0.51 -3.1 1,229,343 - PFR28_01111 0.53 -0.7 1,229,570 + PFR28_01111 0.73 +0.5
Or see this region's nucleotide sequence