Experiment: Choline (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_00458 and PFR28_00459 are separated by 130 nucleotides PFR28_00459 and PFR28_00460 overlap by 1 nucleotides PFR28_00460 and PFR28_00461 overlap by 11 nucleotides
PFR28_00458: PFR28_00458 - hypothetical protein, at 495,898 to 496,938
_00458
PFR28_00459: PFR28_00459 - hypothetical protein, at 497,069 to 497,578
_00459
PFR28_00460: PFR28_00460 - hypothetical protein, at 497,578 to 497,976
_00460
PFR28_00461: PFR28_00461 - hypothetical protein, at 497,966 to 499,006
_00461
Position (kb) 497
498 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 496.136 kb on - strand, within PFR28_00458 at 496.136 kb on - strand, within PFR28_00458 at 496.286 kb on + strand, within PFR28_00458 at 496.286 kb on + strand, within PFR28_00458 at 496.508 kb on - strand, within PFR28_00458 at 496.508 kb on - strand, within PFR28_00458 at 496.508 kb on - strand, within PFR28_00458 at 496.508 kb on - strand, within PFR28_00458 at 496.508 kb on - strand, within PFR28_00458 at 496.538 kb on + strand, within PFR28_00458 at 496.538 kb on + strand, within PFR28_00458 at 496.733 kb on - strand, within PFR28_00458 at 496.790 kb on + strand, within PFR28_00458 at 496.791 kb on - strand, within PFR28_00458 at 496.849 kb on + strand at 496.902 kb on + strand at 496.902 kb on + strand at 496.903 kb on - strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.964 kb on + strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 496.965 kb on - strand at 497.013 kb on - strand at 497.199 kb on - strand, within PFR28_00459 at 497.199 kb on - strand, within PFR28_00459 at 497.339 kb on + strand, within PFR28_00459 at 497.340 kb on - strand, within PFR28_00459 at 497.489 kb on - strand, within PFR28_00459 at 497.532 kb on + strand at 497.800 kb on + strand, within PFR28_00460 at 497.800 kb on + strand, within PFR28_00460 at 497.800 kb on + strand, within PFR28_00460 at 497.800 kb on + strand, within PFR28_00460 at 497.965 kb on + strand at 497.965 kb on + strand at 497.966 kb on - strand at 497.966 kb on - strand at 497.966 kb on - strand at 497.966 kb on - strand at 497.966 kb on - strand at 498.096 kb on - strand, within PFR28_00461 at 498.102 kb on - strand, within PFR28_00461 at 498.293 kb on + strand, within PFR28_00461 at 498.294 kb on - strand, within PFR28_00461 at 498.294 kb on - strand, within PFR28_00461 at 498.294 kb on - strand, within PFR28_00461 at 498.294 kb on - strand, within PFR28_00461 at 498.294 kb on - strand, within PFR28_00461 at 498.515 kb on + strand, within PFR28_00461 at 498.552 kb on - strand, within PFR28_00461 at 498.552 kb on - strand, within PFR28_00461
Per-strain Table
Position Strand Gene LocusTag Fraction Choline (C) 10 mM remove 496,136 - PFR28_00458 0.23 -2.2 496,136 - PFR28_00458 0.23 -1.2 496,286 + PFR28_00458 0.37 -0.1 496,286 + PFR28_00458 0.37 -2.2 496,508 - PFR28_00458 0.59 +0.4 496,508 - PFR28_00458 0.59 -1.6 496,508 - PFR28_00458 0.59 -0.1 496,508 - PFR28_00458 0.59 +1.1 496,508 - PFR28_00458 0.59 -0.4 496,538 + PFR28_00458 0.61 +1.4 496,538 + PFR28_00458 0.61 -3.5 496,733 - PFR28_00458 0.80 -0.5 496,790 + PFR28_00458 0.86 +0.0 496,791 - PFR28_00458 0.86 -1.2 496,849 + +2.1 496,902 + -1.1 496,902 + +0.6 496,903 - -0.0 496,964 + -0.1 496,964 + -0.9 496,964 + -2.0 496,964 + +0.7 496,964 + -0.2 496,964 + +1.2 496,964 + +0.8 496,964 + -0.1 496,964 + +0.7 496,964 + +0.3 496,965 - +0.1 496,965 - -0.1 496,965 - +0.8 496,965 - -0.0 496,965 - -0.1 496,965 - -0.8 496,965 - -0.9 496,965 - -0.1 496,965 - -0.6 497,013 - -1.0 497,199 - PFR28_00459 0.25 -0.4 497,199 - PFR28_00459 0.25 +0.0 497,339 + PFR28_00459 0.53 -0.3 497,340 - PFR28_00459 0.53 +0.2 497,489 - PFR28_00459 0.82 -0.9 497,532 + -2.2 497,800 + PFR28_00460 0.56 -0.3 497,800 + PFR28_00460 0.56 -2.2 497,800 + PFR28_00460 0.56 +0.2 497,800 + PFR28_00460 0.56 -0.1 497,965 + -3.4 497,965 + +2.6 497,966 - -1.7 497,966 - -1.4 497,966 - +0.4 497,966 - -2.0 497,966 - -0.2 498,096 - PFR28_00461 0.12 -1.2 498,102 - PFR28_00461 0.13 +0.7 498,293 + PFR28_00461 0.31 -0.4 498,294 - PFR28_00461 0.32 +1.1 498,294 - PFR28_00461 0.32 +0.3 498,294 - PFR28_00461 0.32 -0.2 498,294 - PFR28_00461 0.32 -1.0 498,294 - PFR28_00461 0.32 -1.2 498,515 + PFR28_00461 0.53 +1.4 498,552 - PFR28_00461 0.56 +0.3 498,552 - PFR28_00461 0.56 -0.9
Or see this region's nucleotide sequence