Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22480 and AMB_RS22485 overlap by 1 nucleotides AMB_RS22485 and AMB_RS22490 overlap by 4 nucleotides AMB_RS22490 and AMB_RS22495 overlap by 4 nucleotides
AMB_RS22480: AMB_RS22480 - cell division ATP-binding protein FtsE, at 4,855,476 to 4,856,198
_RS22480
AMB_RS22485: AMB_RS22485 - cell division protein, at 4,856,198 to 4,857,094
_RS22485
AMB_RS22490: AMB_RS22490 - YdcF family protein, at 4,857,091 to 4,857,711
_RS22490
AMB_RS22495: AMB_RS22495 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 4,857,708 to 4,858,439
_RS22495
Position (kb)
4857
4858 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4856.840 kb on + strand, within AMB_RS22485 at 4856.840 kb on + strand, within AMB_RS22485 at 4856.999 kb on + strand, within AMB_RS22485 at 4857.643 kb on + strand, within AMB_RS22490 at 4857.644 kb on - strand, within AMB_RS22490 at 4857.644 kb on - strand, within AMB_RS22490 at 4857.653 kb on - strand at 4858.445 kb on + strand at 4858.445 kb on + strand at 4858.483 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.563 kb on + strand at 4858.563 kb on + strand at 4858.566 kb on - strand at 4858.566 kb on - strand at 4858.672 kb on + strand at 4858.672 kb on + strand at 4858.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 4,856,840 + AMB_RS22485 0.72 +0.8 4,856,840 + AMB_RS22485 0.72 -1.6 4,856,999 + AMB_RS22485 0.89 -0.1 4,857,643 + AMB_RS22490 0.89 -0.5 4,857,644 - AMB_RS22490 0.89 -0.8 4,857,644 - AMB_RS22490 0.89 -0.8 4,857,653 - -1.0 4,858,445 + +0.2 4,858,445 + -0.6 4,858,483 - +0.2 4,858,548 - -2.3 4,858,548 - +1.8 4,858,548 - -0.5 4,858,548 - -0.1 4,858,548 - -0.8 4,858,563 + -0.8 4,858,563 + -1.4 4,858,566 - +0.2 4,858,566 - -0.2 4,858,672 + -0.6 4,858,672 + -3.1 4,858,673 - -0.0
Or see this region's nucleotide sequence