Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22000 and AMB_RS22005 overlap by 4 nucleotides AMB_RS22005 and AMB_RS22010 are separated by 36 nucleotides AMB_RS22010 and AMB_RS22015 are separated by 109 nucleotides
AMB_RS22000: AMB_RS22000 - MFS transporter, at 4,753,939 to 4,755,150
_RS22000
AMB_RS22005: AMB_RS22005 - HD domain-containing protein, at 4,755,147 to 4,755,746
_RS22005
AMB_RS22010: AMB_RS22010 - hypothetical protein, at 4,755,783 to 4,756,193
_RS22010
AMB_RS22015: AMB_RS22015 - methyltransferase domain-containing protein, at 4,756,303 to 4,757,073
_RS22015
Position (kb) 4755
4756
4757 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4755.120 kb on + strand at 4755.235 kb on + strand, within AMB_RS22005 at 4755.235 kb on + strand, within AMB_RS22005 at 4755.236 kb on - strand, within AMB_RS22005 at 4755.236 kb on - strand, within AMB_RS22005 at 4755.316 kb on - strand, within AMB_RS22005 at 4755.520 kb on - strand, within AMB_RS22005 at 4755.693 kb on + strand at 4755.693 kb on + strand at 4755.694 kb on - strand at 4755.732 kb on + strand at 4755.732 kb on + strand at 4755.732 kb on + strand at 4755.733 kb on - strand at 4755.745 kb on - strand at 4755.784 kb on + strand at 4756.142 kb on - strand, within AMB_RS22010 at 4756.142 kb on - strand, within AMB_RS22010 at 4756.142 kb on - strand, within AMB_RS22010 at 4756.226 kb on + strand at 4756.227 kb on - strand at 4756.227 kb on - strand at 4756.398 kb on - strand, within AMB_RS22015 at 4756.398 kb on - strand, within AMB_RS22015 at 4756.511 kb on + strand, within AMB_RS22015 at 4756.512 kb on - strand, within AMB_RS22015 at 4756.512 kb on - strand, within AMB_RS22015 at 4756.512 kb on - strand, within AMB_RS22015 at 4756.533 kb on + strand, within AMB_RS22015 at 4756.562 kb on + strand, within AMB_RS22015 at 4756.562 kb on + strand, within AMB_RS22015 at 4756.562 kb on + strand, within AMB_RS22015 at 4756.563 kb on - strand, within AMB_RS22015 at 4756.563 kb on - strand, within AMB_RS22015 at 4756.563 kb on - strand, within AMB_RS22015 at 4756.565 kb on + strand, within AMB_RS22015 at 4756.565 kb on + strand, within AMB_RS22015 at 4756.565 kb on + strand, within AMB_RS22015 at 4756.566 kb on - strand, within AMB_RS22015 at 4756.566 kb on - strand, within AMB_RS22015 at 4756.566 kb on - strand, within AMB_RS22015 at 4756.566 kb on - strand, within AMB_RS22015 at 4756.566 kb on - strand, within AMB_RS22015 at 4756.670 kb on + strand, within AMB_RS22015 at 4756.670 kb on + strand, within AMB_RS22015 at 4756.671 kb on - strand, within AMB_RS22015 at 4756.671 kb on - strand, within AMB_RS22015 at 4756.929 kb on + strand, within AMB_RS22015 at 4756.930 kb on - strand, within AMB_RS22015 at 4756.935 kb on + strand, within AMB_RS22015 at 4756.935 kb on + strand, within AMB_RS22015 at 4757.051 kb on + strand at 4757.052 kb on - strand at 4757.052 kb on - strand at 4757.127 kb on + strand at 4757.128 kb on - strand at 4757.144 kb on + strand at 4757.145 kb on - strand at 4757.145 kb on - strand at 4757.145 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 4,755,120 + -3.4 4,755,235 + AMB_RS22005 0.15 -0.2 4,755,235 + AMB_RS22005 0.15 +0.8 4,755,236 - AMB_RS22005 0.15 -0.2 4,755,236 - AMB_RS22005 0.15 +1.3 4,755,316 - AMB_RS22005 0.28 -2.2 4,755,520 - AMB_RS22005 0.62 -2.8 4,755,693 + -0.1 4,755,693 + +0.6 4,755,694 - -1.8 4,755,732 + -1.6 4,755,732 + -1.0 4,755,732 + -0.1 4,755,733 - +0.1 4,755,745 - -1.4 4,755,784 + +0.8 4,756,142 - AMB_RS22010 0.87 -0.2 4,756,142 - AMB_RS22010 0.87 -1.8 4,756,142 - AMB_RS22010 0.87 -1.7 4,756,226 + +0.1 4,756,227 - +0.2 4,756,227 - +1.5 4,756,398 - AMB_RS22015 0.12 +0.8 4,756,398 - AMB_RS22015 0.12 +1.8 4,756,511 + AMB_RS22015 0.27 -1.9 4,756,512 - AMB_RS22015 0.27 +0.4 4,756,512 - AMB_RS22015 0.27 -1.4 4,756,512 - AMB_RS22015 0.27 -3.1 4,756,533 + AMB_RS22015 0.30 +1.8 4,756,562 + AMB_RS22015 0.34 +0.2 4,756,562 + AMB_RS22015 0.34 +0.6 4,756,562 + AMB_RS22015 0.34 -0.4 4,756,563 - AMB_RS22015 0.34 -0.2 4,756,563 - AMB_RS22015 0.34 -1.4 4,756,563 - AMB_RS22015 0.34 +1.0 4,756,565 + AMB_RS22015 0.34 +1.4 4,756,565 + AMB_RS22015 0.34 -0.6 4,756,565 + AMB_RS22015 0.34 -0.6 4,756,566 - AMB_RS22015 0.34 -1.4 4,756,566 - AMB_RS22015 0.34 -1.0 4,756,566 - AMB_RS22015 0.34 -0.0 4,756,566 - AMB_RS22015 0.34 -0.6 4,756,566 - AMB_RS22015 0.34 -0.8 4,756,670 + AMB_RS22015 0.48 -0.2 4,756,670 + AMB_RS22015 0.48 -1.6 4,756,671 - AMB_RS22015 0.48 -0.2 4,756,671 - AMB_RS22015 0.48 +0.3 4,756,929 + AMB_RS22015 0.81 +0.2 4,756,930 - AMB_RS22015 0.81 +0.3 4,756,935 + AMB_RS22015 0.82 -0.6 4,756,935 + AMB_RS22015 0.82 +0.4 4,757,051 + +1.0 4,757,052 - -1.0 4,757,052 - -0.6 4,757,127 + -0.0 4,757,128 - +0.1 4,757,144 + -1.6 4,757,145 - +0.2 4,757,145 - -0.6 4,757,145 - +0.6
Or see this region's nucleotide sequence