Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS19335 and AMB_RS19340 overlap by 4 nucleotides AMB_RS19340 and AMB_RS19345 overlap by 1 nucleotides AMB_RS19345 and AMB_RS19350 are separated by 139 nucleotides AMB_RS19350 and AMB_RS19355 are separated by 129 nucleotides
AMB_RS19335: AMB_RS19335 - ATPase, at 4,220,232 to 4,221,029
_RS19335
AMB_RS19340: AMB_RS19340 - EamA family transporter, at 4,221,026 to 4,221,553
_RS19340
AMB_RS19345: AMB_RS19345 - chemotaxis sensory transducer, at 4,221,553 to 4,222,605
_RS19345
AMB_RS19350: AMB_RS19350 - RNA polymerase-binding protein DksA, at 4,222,745 to 4,223,161
_RS19350
AMB_RS19355: AMB_RS19355 - flagellar assembly protein FliX, at 4,223,291 to 4,223,722
_RS19355
Position (kb) 4221
4222
4223 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4221.201 kb on + strand, within AMB_RS19340 at 4221.202 kb on - strand, within AMB_RS19340 at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.501 kb on + strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.502 kb on - strand at 4221.605 kb on + strand at 4221.623 kb on + strand at 4221.623 kb on + strand at 4221.624 kb on - strand at 4221.624 kb on - strand at 4221.624 kb on - strand at 4221.624 kb on - strand at 4221.624 kb on - strand at 4221.633 kb on - strand at 4221.651 kb on - strand at 4221.651 kb on - strand at 4221.680 kb on + strand, within AMB_RS19345 at 4221.681 kb on - strand, within AMB_RS19345 at 4221.681 kb on - strand, within AMB_RS19345 at 4221.681 kb on - strand, within AMB_RS19345 at 4221.681 kb on - strand, within AMB_RS19345 at 4221.926 kb on + strand, within AMB_RS19345 at 4221.926 kb on + strand, within AMB_RS19345 at 4221.926 kb on + strand, within AMB_RS19345 at 4221.927 kb on - strand, within AMB_RS19345 at 4221.927 kb on - strand, within AMB_RS19345 at 4221.927 kb on - strand, within AMB_RS19345 at 4221.927 kb on - strand, within AMB_RS19345 at 4221.983 kb on + strand, within AMB_RS19345 at 4221.984 kb on - strand, within AMB_RS19345 at 4222.124 kb on + strand, within AMB_RS19345 at 4222.124 kb on + strand, within AMB_RS19345 at 4222.125 kb on - strand, within AMB_RS19345 at 4222.125 kb on - strand, within AMB_RS19345 at 4222.125 kb on - strand, within AMB_RS19345 at 4222.125 kb on - strand, within AMB_RS19345 at 4222.133 kb on + strand, within AMB_RS19345 at 4222.256 kb on + strand, within AMB_RS19345 at 4222.256 kb on + strand, within AMB_RS19345 at 4222.257 kb on - strand, within AMB_RS19345 at 4222.506 kb on + strand at 4222.726 kb on + strand at 4222.727 kb on - strand at 4222.730 kb on + strand at 4223.089 kb on - strand, within AMB_RS19350 at 4223.206 kb on - strand at 4223.208 kb on - strand at 4223.208 kb on - strand at 4223.266 kb on + strand at 4223.266 kb on + strand at 4223.267 kb on - strand at 4223.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 4,221,201 + AMB_RS19340 0.33 -3.7 4,221,202 - AMB_RS19340 0.33 +1.6 4,221,501 + -1.2 4,221,501 + -0.8 4,221,501 + -1.6 4,221,501 + -0.8 4,221,501 + -1.0 4,221,501 + -1.0 4,221,501 + -0.8 4,221,501 + -0.8 4,221,501 + -1.4 4,221,501 + -0.0 4,221,502 - -0.4 4,221,502 - -0.2 4,221,502 - +0.2 4,221,502 - -0.8 4,221,502 - +0.6 4,221,502 - -0.4 4,221,502 - -0.3 4,221,502 - -2.2 4,221,605 + -1.4 4,221,623 + +0.2 4,221,623 + +0.4 4,221,624 - +0.1 4,221,624 - +0.4 4,221,624 - -1.0 4,221,624 - +0.6 4,221,624 - -0.0 4,221,633 - +1.0 4,221,651 - -0.1 4,221,651 - +1.0 4,221,680 + AMB_RS19345 0.12 -1.4 4,221,681 - AMB_RS19345 0.12 +0.4 4,221,681 - AMB_RS19345 0.12 +0.4 4,221,681 - AMB_RS19345 0.12 -0.4 4,221,681 - AMB_RS19345 0.12 -0.5 4,221,926 + AMB_RS19345 0.35 -1.6 4,221,926 + AMB_RS19345 0.35 -0.8 4,221,926 + AMB_RS19345 0.35 -1.0 4,221,927 - AMB_RS19345 0.36 -0.4 4,221,927 - AMB_RS19345 0.36 +0.4 4,221,927 - AMB_RS19345 0.36 -1.0 4,221,927 - AMB_RS19345 0.36 -2.2 4,221,983 + AMB_RS19345 0.41 -0.2 4,221,984 - AMB_RS19345 0.41 +0.6 4,222,124 + AMB_RS19345 0.54 -4.3 4,222,124 + AMB_RS19345 0.54 +1.1 4,222,125 - AMB_RS19345 0.54 +0.6 4,222,125 - AMB_RS19345 0.54 +0.0 4,222,125 - AMB_RS19345 0.54 +0.7 4,222,125 - AMB_RS19345 0.54 +0.4 4,222,133 + AMB_RS19345 0.55 +0.3 4,222,256 + AMB_RS19345 0.67 -0.6 4,222,256 + AMB_RS19345 0.67 -0.8 4,222,257 - AMB_RS19345 0.67 -0.3 4,222,506 + +1.6 4,222,726 + -0.1 4,222,727 - -0.1 4,222,730 + -1.6 4,223,089 - AMB_RS19350 0.82 -3.2 4,223,206 - +0.3 4,223,208 - -1.2 4,223,208 - -2.2 4,223,266 + -0.1 4,223,266 + +0.6 4,223,267 - -1.9 4,223,267 - +0.1
Or see this region's nucleotide sequence