Experiment: 30_an_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS15325 and AMB_RS15330 are separated by 12 nucleotides AMB_RS15330 and AMB_RS15335 are separated by 13 nucleotides AMB_RS15335 and AMB_RS15340 overlap by 4 nucleotides AMB_RS15340 and AMB_RS15345 are separated by 160 nucleotides
AMB_RS15325: AMB_RS15325 - tungsten ABC transporter permease, at 3,283,017 to 3,283,868
_RS15325
AMB_RS15330: AMB_RS15330 - tungsten ABC transporter substrate-binding protein, at 3,283,881 to 3,284,690
_RS15330
AMB_RS15335: AMB_RS15335 - ABC transporter ATP-binding protein, at 3,284,704 to 3,285,432
_RS15335
AMB_RS15340: AMB_RS15340 - ABC transporter permease, at 3,285,429 to 3,286,133
_RS15340
AMB_RS15345: AMB_RS15345 - 30S ribosomal protein S12 methylthiotransferase RimO, at 3,286,294 to 3,287,640
_RS15345
Position (kb)
3284
3285
3286 Strain fitness (log2 ratio)
-2
-1
0
1 at 3283.882 kb on + strand at 3283.882 kb on + strand at 3283.883 kb on - strand at 3283.883 kb on - strand at 3283.883 kb on - strand at 3283.883 kb on - strand at 3283.883 kb on - strand at 3283.883 kb on - strand at 3284.129 kb on - strand, within AMB_RS15330 at 3284.386 kb on + strand, within AMB_RS15330 at 3284.386 kb on + strand, within AMB_RS15330 at 3284.386 kb on + strand, within AMB_RS15330 at 3284.386 kb on + strand, within AMB_RS15330 at 3284.386 kb on + strand, within AMB_RS15330 at 3284.387 kb on - strand, within AMB_RS15330 at 3284.387 kb on - strand, within AMB_RS15330 at 3284.387 kb on - strand, within AMB_RS15330 at 3284.387 kb on - strand, within AMB_RS15330 at 3285.131 kb on + strand, within AMB_RS15335 at 3285.745 kb on + strand, within AMB_RS15340 at 3285.745 kb on + strand, within AMB_RS15340 at 3285.745 kb on + strand, within AMB_RS15340 at 3285.745 kb on + strand, within AMB_RS15340 at 3285.746 kb on - strand, within AMB_RS15340 at 3285.746 kb on - strand, within AMB_RS15340 at 3285.976 kb on - strand, within AMB_RS15340 at 3286.152 kb on + strand at 3286.153 kb on - strand at 3286.153 kb on - strand at 3286.153 kb on - strand at 3286.411 kb on + strand at 3286.411 kb on + strand at 3286.411 kb on + strand at 3286.411 kb on + strand at 3286.412 kb on - strand at 3286.412 kb on - strand at 3286.412 kb on - strand at 3286.412 kb on - strand at 3286.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_2 remove 3,283,882 + +0.5 3,283,882 + +0.1 3,283,883 - -0.1 3,283,883 - +0.5 3,283,883 - -0.7 3,283,883 - -2.1 3,283,883 - -0.3 3,283,883 - -0.5 3,284,129 - AMB_RS15330 0.31 -0.2 3,284,386 + AMB_RS15330 0.62 -0.1 3,284,386 + AMB_RS15330 0.62 -0.2 3,284,386 + AMB_RS15330 0.62 +1.5 3,284,386 + AMB_RS15330 0.62 -1.3 3,284,386 + AMB_RS15330 0.62 -0.5 3,284,387 - AMB_RS15330 0.62 -0.3 3,284,387 - AMB_RS15330 0.62 -1.5 3,284,387 - AMB_RS15330 0.62 +0.1 3,284,387 - AMB_RS15330 0.62 -0.1 3,285,131 + AMB_RS15335 0.59 +0.4 3,285,745 + AMB_RS15340 0.45 -2.4 3,285,745 + AMB_RS15340 0.45 +0.6 3,285,745 + AMB_RS15340 0.45 -0.4 3,285,745 + AMB_RS15340 0.45 +1.0 3,285,746 - AMB_RS15340 0.45 +0.3 3,285,746 - AMB_RS15340 0.45 -1.7 3,285,976 - AMB_RS15340 0.78 +0.0 3,286,152 + +0.0 3,286,153 - +0.5 3,286,153 - +0.5 3,286,153 - -0.2 3,286,411 + +0.1 3,286,411 + -1.8 3,286,411 + +0.4 3,286,411 + -0.8 3,286,412 - +0.7 3,286,412 - +0.3 3,286,412 - -0.2 3,286,412 - -0.4 3,286,412 - +0.7
Or see this region's nucleotide sequence